Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV
Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. Analytical pre-requi...
Saved in:
| Published in | Clinical biochemistry Vol. 46; no. 7-8; pp. 665 - 669 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Elsevier Inc
01.05.2013
|
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma.
Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements.
Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase.
Results indicated an excellent linearity with correlation coefficients (r2)≥0.999. The LOD of AA and UA was 1.02 and 1.42nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA.
This validated method allows the determination of both antioxidants within 10min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects.
[Display omitted]
► Rapid and simultaneous determination of ascorbic and uric acids levels in plasma. ► The use of heparin as anticoagulant and MPA as stabilizer is required. ► Ion-exclusion HPLC-UV isocratic elution within 10min. ► Method offers an excellent precision and recovery for ascorbic and uric acids. ► Methodology suitable for high-throughput clinical analysis. |
|---|---|
| AbstractList | Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma.OBJECTIVESAscorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma.Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase.METHODSAnalytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase.Results indicated an excellent linearity with correlation coefficients (r(2))≥0.999. The LOD of AA and UA was 1.02 and 1.42nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA.RESULTSResults indicated an excellent linearity with correlation coefficients (r(2))≥0.999. The LOD of AA and UA was 1.02 and 1.42nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA.This validated method allows the determination of both antioxidants within 10min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects.CONCLUSIONThis validated method allows the determination of both antioxidants within 10min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects. Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase. Results indicated an excellent linearity with correlation coefficients (r(2))≥0.999. The LOD of AA and UA was 1.02 and 1.42nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA. This validated method allows the determination of both antioxidants within 10min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects. Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid, sensitive, and accurate ion-exclusion HPLC-UV methodology for the simultaneously determination of AA and UA in human plasma. Analytical pre-requisites, such as the use of heparin as an anticoagulant and meta-phosphoric acid as a stabilizer were added for accurate and reliable measurements. Chromatographic separation was achieved by an isocratic elution on a HEMA-BIO 1000 SB analytical column using a phosphate buffer, pH 2.4, as a mobile phase. Results indicated an excellent linearity with correlation coefficients (r2)≥0.999. The LOD of AA and UA was 1.02 and 1.42nmol/mL, respectively, while LOQ ranged from 0.306 to 0.426nmol/mL. A great repeatability for both antioxidants was found, where the CV (%) values for intra-day were lower than 1.8% and under 6.5% for the inter-day assay. The recovery of AA ranged from 92% to 96% and from 99% to 100% for UA. This validated method allows the determination of both antioxidants within 10min, and is well suited to routine measurements and/or high-throughput clinical analysis. The methodology was applied to assess the antioxidant status of a group of Azorean subjects. [Display omitted] ► Rapid and simultaneous determination of ascorbic and uric acids levels in plasma. ► The use of heparin as anticoagulant and MPA as stabilizer is required. ► Ion-exclusion HPLC-UV isocratic elution within 10min. ► Method offers an excellent precision and recovery for ascorbic and uric acids. ► Methodology suitable for high-throughput clinical analysis. |
| Author | Ferin, Rita Baptista, José Pavão, Maria Leonor |
| Author_xml | – sequence: 1 givenname: Rita surname: Ferin fullname: Ferin, Rita email: rborges@uac.pt organization: Department of Technological Sciences and Development, University of the Azores, Rua da Mãe de Deus, 9501-801 Ponta Delgada, Azores, Portugal – sequence: 2 givenname: Maria Leonor surname: Pavão fullname: Pavão, Maria Leonor email: lpavao@uac.pt organization: Department of Technological Sciences and Development, University of the Azores, Rua da Mãe de Deus, 9501-801 Ponta Delgada, Azores, Portugal – sequence: 3 givenname: José surname: Baptista fullname: Baptista, José email: baptista@uac.pt organization: Department of Technological Sciences and Development, University of the Azores, Rua da Mãe de Deus, 9501-801 Ponta Delgada, Azores, Portugal |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23353131$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkd1LHDEUxYNY6mr9FyR960Nnmg_nI09SlvoBC4pUX0MmuYN3mUnWJCP1v--sa6X45NO9F37nwD3nkOz74IGQr5yVnPH6x7q0A_oOg32AsRSMy5LxkrF6jyx428hCKCn3yYIxpgrFBTsghymt51OctvVnciCkrCSXfEHSrdmg-04T-IQZn4Aa72jCcRqy8RCmRB1kiCN6kzF4Gnpqkg2xQ_uCTnG7WHSJoqcP02g83QwmjYZ2z3RWFPDHDlPaai9vVsvi7v4L-dSbIcHx6zwid-e_fi8vi9X1xdXy56qwp6LNhWp7YUXdK5BWCWgcKME7WQvpTC_rhhnunOhAVpUyvG-rpgZbVaJvmBSKSXlEvu18NzE8TpCyHjFZGIbdY5pL0TSimjOZ0ZNXdOpGcHoTcTTxWf8LagbUDrAxpBShf0M409tS9Fr_V4relqIZ13Mps_bsndZifkkzR4PDhxyWOweY43pCiDpZBG_BYQSbtQv4AZe_ur-wlA |
| CitedBy_id | crossref_primary_10_1021_acsami_7b01675 crossref_primary_10_1039_C9RA06481H crossref_primary_10_3390_separations9100282 crossref_primary_10_1134_S1023193518130347 crossref_primary_10_1016_j_inoche_2021_108627 crossref_primary_10_1016_j_arabjc_2024_105692 crossref_primary_10_1038_srep16011 crossref_primary_10_1016_j_msec_2016_03_082 crossref_primary_10_1155_2013_854041 crossref_primary_10_1149_2_1191614jes crossref_primary_10_1002_jssc_201601243 crossref_primary_10_1155_2024_9944426 crossref_primary_10_1016_j_saa_2016_11_031 crossref_primary_10_1016_j_jfca_2024_106673 crossref_primary_10_1016_j_sna_2015_09_013 crossref_primary_10_1080_00032719_2014_930870 crossref_primary_10_1016_j_electacta_2020_137631 crossref_primary_10_1016_j_chroma_2017_05_036 crossref_primary_10_1016_j_foodchem_2016_09_025 crossref_primary_10_1134_S1061934820090038 crossref_primary_10_1007_s13738_019_01758_4 crossref_primary_10_1080_10408347_2019_1637711 crossref_primary_10_1016_j_jtemb_2020_126701 crossref_primary_10_1016_j_snb_2015_10_101 crossref_primary_10_1016_j_talanta_2014_08_066 crossref_primary_10_1002_jssc_201501038 crossref_primary_10_1016_j_foodchem_2021_129977 crossref_primary_10_1016_j_arabjc_2014_02_006 crossref_primary_10_1016_j_jchromb_2015_08_044 crossref_primary_10_1007_s42250_018_0001_0 crossref_primary_10_1016_j_snb_2021_130141 crossref_primary_10_1007_s40242_017_6473_7 crossref_primary_10_1515_cclm_2021_0614 crossref_primary_10_1002_bmc_3529 crossref_primary_10_1016_j_jchromb_2021_122588 crossref_primary_10_1002_bmc_3687 crossref_primary_10_1186_s12937_016_0158_9 crossref_primary_10_1002_elan_201700043 crossref_primary_10_1016_j_microc_2018_08_003 |
| Cites_doi | 10.1016/j.foodchem.2005.09.023 10.1016/j.cca.2005.09.002 10.2174/138161205774913255 10.1016/j.jchromb.2009.02.008 10.1093/jn/137.10.2171 10.1016/j.foodchem.2007.06.060 10.1038/35025203 10.1093/qjmed/93.11.707 10.1093/clinchem/42.4.498 10.1038/sj.ejcn.1602655 10.1016/S0378-4347(96)00297-6 10.1111/j.1742-7843.2009.00420.x 10.1016/j.abb.2010.05.014 10.1002/bmc.787 10.1007/BF00163854 10.1001/jama.281.15.1415 10.1093/ajcn/54.4.712 10.1016/j.pathophys.2006.05.002 10.1016/j.jchromb.2005.07.008 10.1016/S1520-765X(02)90011-8 10.1016/j.jchromb.2009.05.037 10.1016/j.numecd.2007.02.011 10.1016/S0009-9120(01)00270-3 10.1056/NEJM199708073370607 10.1016/j.trac.2008.08.006 10.1016/j.bcp.2008.09.024 10.1006/abio.1998.2773 10.1016/j.jbbm.2006.01.006 10.1016/j.nutres.2011.11.005 |
| ContentType | Journal Article |
| Copyright | 2013 The Canadian Society of Clinical Chemists Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
| Copyright_xml | – notice: 2013 The Canadian Society of Clinical Chemists – notice: Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7X8 |
| DOI | 10.1016/j.clinbiochem.2013.01.006 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine Chemistry |
| EISSN | 1873-2933 |
| EndPage | 669 |
| ExternalDocumentID | 23353131 10_1016_j_clinbiochem_2013_01_006 S000991201300012X |
| Genre | Validation Studies Journal Article |
| GroupedDBID | --- --K --M -~X .55 .GJ .~1 0R~ 1B1 1RT 1~. 1~5 29B 4.4 457 4G. 4R4 53G 5GY 5VS 6J9 7-5 71M 8P~ 9JM AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO AAYJJ ABBQC ABFNM ABFRF ABGSF ABJNI ABLVK ABMAC ABMZM ABOCM ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHPSJ AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q G8K GBLVA HLW HVGLF HX~ HZ~ IHE J1W KOM LCYCR LX3 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SBG SDF SDG SDP SES SEW SPCBC SSH SSU SSZ T5K UNMZH WUQ X7M XPP YYP YYQ ZGI ZUP ~G- AATTM AAXKI AAYWO AAYXX ABDPE ABWVN ACIEU ACRPL ACVFH ADCNI ADNMO ADVLN AEIPS AEUPX AFJKZ AFPUW AGCQF AGQPQ AGRNS AIGII AIIUN AKBMS AKRWK AKYEP ANKPU APXCP CITATION CGR CUY CVF ECM EFKBS EIF NPM 7X8 |
| ID | FETCH-LOGICAL-c428t-98f2c26f9e3c92e7de921b3623daf3670a1dd2be3559a1f8576ec552f70329033 |
| IEDL.DBID | .~1 |
| ISSN | 0009-9120 1873-2933 |
| IngestDate | Thu Jul 10 23:22:14 EDT 2025 Mon Jul 21 06:03:24 EDT 2025 Tue Jul 01 01:11:33 EDT 2025 Thu Apr 24 22:52:01 EDT 2025 Fri Feb 23 02:36:34 EST 2024 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 7-8 |
| Keywords | Ion-exclusion Ascorbic acid Uric acid Plasma antioxidants HPLC-UV Atherosclerosis |
| Language | English |
| License | Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c428t-98f2c26f9e3c92e7de921b3623daf3670a1dd2be3559a1f8576ec552f70329033 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Undefined-3 |
| OpenAccessLink | https://ars.els-cdn.com/content/image/1-s2.0-S000991201300012X-fx1.jpg |
| PMID | 23353131 |
| PQID | 1327725248 |
| PQPubID | 23479 |
| PageCount | 5 |
| ParticipantIDs | proquest_miscellaneous_1327725248 pubmed_primary_23353131 crossref_primary_10_1016_j_clinbiochem_2013_01_006 crossref_citationtrail_10_1016_j_clinbiochem_2013_01_006 elsevier_sciencedirect_doi_10_1016_j_clinbiochem_2013_01_006 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | May 2013 2013-05-00 2013-May 20130501 |
| PublicationDateYYYYMMDD | 2013-05-01 |
| PublicationDate_xml | – month: 05 year: 2013 text: May 2013 |
| PublicationDecade | 2010 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Clinical biochemistry |
| PublicationTitleAlternate | Clin Biochem |
| PublicationYear | 2013 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Violi, Micheletta, Iuliano (bb0025) 2002; 4 Glantzounis, Tsimoyiannis, Kappas, Galaris (bb0065) 2005; 11 Jialal, Devaraj (bb0015) 1996; 42 Lykkesfeldt (bb0110) 2012; 32 Verrax, Buc Calderon (bb0035) 2008; 76 Yao, Wang, Chen (bb0095) 2007; 21 Nováková, Solich, Solichová (bb0140) 2008; 27 Lusis (bb0005) 2000; 407 Kafkas, Koşar, Türemiş, Başer (bb0115) 2006; 97 Dhariwal, Hartzell, Levine (bb0100) 1991; 54 Causon (bb0130) 1997; 689 Parviainen, Nyyssonen (bb0170) 1992 Corti, Casini, Pompella (bb0150) 2010; 500 Wu, Muzny, Chi Lee, Thomas Caskey (bb0070) 1992; 34 Kand'ár, Žáková, Mužáková (bb0160) 2006; 365 Li, Franke (bb0165) 2009; 877 Padayatty, Levine (bb0040) 2001; 164 Strazzullo, Puig (bb0060) 2007; 17 Romeu-Nadal, Castellote, Gayą, López-Sabater (bb0120) 2008; 107 Pappa-Louisi, Pascalidou (bb0085) 1998; 263 Zinellu, Sotgia, Deiana, Carru (bb0090) 2006; 67 Chung, Chung, Szeto, Tomlinson, Benzie (bb0135) 2001; 34 Waring, Webb, Maxwell (bb0155) 2000; 93 Frikke-Schmidt, Lykkesfeldt (bb0050) 2009; 104 Karlsen, Blomhoff, Gundersen (bb0105) 2007; 61 Epstein, Diaz, Frei, Vita, Keaney (bb0020) 1997; 337 Karlsen, Blomhoff, Gundersen (bb0125) 2005; 824 Levine, Rumsey, Daruwala, Park, Wang (bb0030) 1999; 281 Singh, Jialal (bb0010) 2006; 13 Li, Schellhorn (bb0045) 2007; 137 Kim, Henderson, Ouyang (bb0055) 2009; 877 Verrax (10.1016/j.clinbiochem.2013.01.006_bb0035) 2008; 76 Glantzounis (10.1016/j.clinbiochem.2013.01.006_bb0065) 2005; 11 Karlsen (10.1016/j.clinbiochem.2013.01.006_bb0125) 2005; 824 Causon (10.1016/j.clinbiochem.2013.01.006_bb0130) 1997; 689 Yao (10.1016/j.clinbiochem.2013.01.006_bb0095) 2007; 21 Li (10.1016/j.clinbiochem.2013.01.006_bb0045) 2007; 137 Chung (10.1016/j.clinbiochem.2013.01.006_bb0135) 2001; 34 Strazzullo (10.1016/j.clinbiochem.2013.01.006_bb0060) 2007; 17 Wu (10.1016/j.clinbiochem.2013.01.006_bb0070) 1992; 34 Violi (10.1016/j.clinbiochem.2013.01.006_bb0025) 2002; 4 Waring (10.1016/j.clinbiochem.2013.01.006_bb0155) 2000; 93 Levine (10.1016/j.clinbiochem.2013.01.006_bb0030) 1999; 281 Romeu-Nadal (10.1016/j.clinbiochem.2013.01.006_bb0120) 2008; 107 Padayatty (10.1016/j.clinbiochem.2013.01.006_bb0040) 2001; 164 Kafkas (10.1016/j.clinbiochem.2013.01.006_bb0115) 2006; 97 Li (10.1016/j.clinbiochem.2013.01.006_bb0165) 2009; 877 Corti (10.1016/j.clinbiochem.2013.01.006_bb0150) 2010; 500 Kand'ár (10.1016/j.clinbiochem.2013.01.006_bb0160) 2006; 365 Kim (10.1016/j.clinbiochem.2013.01.006_bb0055) 2009; 877 Parviainen (10.1016/j.clinbiochem.2013.01.006_bb0170) 1992 Singh (10.1016/j.clinbiochem.2013.01.006_bb0010) 2006; 13 Zinellu (10.1016/j.clinbiochem.2013.01.006_bb0090) 2006; 67 Karlsen (10.1016/j.clinbiochem.2013.01.006_bb0105) 2007; 61 Lykkesfeldt (10.1016/j.clinbiochem.2013.01.006_bb0110) 2012; 32 Dhariwal (10.1016/j.clinbiochem.2013.01.006_bb0100) 1991; 54 Nováková (10.1016/j.clinbiochem.2013.01.006_bb0140) 2008; 27 Lusis (10.1016/j.clinbiochem.2013.01.006_bb0005) 2000; 407 Frikke-Schmidt (10.1016/j.clinbiochem.2013.01.006_bb0050) 2009; 104 Pappa-Louisi (10.1016/j.clinbiochem.2013.01.006_bb0085) 1998; 263 Epstein (10.1016/j.clinbiochem.2013.01.006_bb0020) 1997; 337 Jialal (10.1016/j.clinbiochem.2013.01.006_bb0015) 1996; 42 |
| References_xml | – volume: 164 start-page: 353 year: 2001 end-page: 355 ident: bb0040 article-title: New insights into the physiology and pharmacology of vitamin C publication-title: Can Med Assoc J – volume: 54 start-page: 712 year: 1991 end-page: 716 ident: bb0100 article-title: Ascorbic acid and dehydroascorbic acid measurements in human plasma and serum publication-title: Am J Clin Nutr – volume: 337 start-page: 408 year: 1997 end-page: 416 ident: bb0020 article-title: Antioxidants and atherosclerotic heart disease publication-title: N Engl J Med – volume: 34 start-page: 78 year: 1992 end-page: 84 ident: bb0070 article-title: Two independent mutational events in the loss of urate oxidase during hominoid evolution publication-title: J Mol Evol – volume: 137 start-page: 2171 year: 2007 end-page: 2184 ident: bb0045 article-title: New developments and novel therapeutic perspectives for vitamin C publication-title: J Nutr – volume: 4 start-page: B17 year: 2002 ident: bb0025 article-title: Antioxidants and atherosclerosis publication-title: Eur Heart J Supplements – volume: 32 start-page: 66 year: 2012 end-page: 69 ident: bb0110 article-title: Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used publication-title: Nutr Res – year: 1992 ident: bb0170 article-title: Ascorbic acid publication-title: Modern Chromatographic Analysis of Vitamins – volume: 263 start-page: 176 year: 1998 end-page: 182 ident: bb0085 article-title: Optimal conditions for the simultaneous ion-pairing HPLC determination ofl-ascorbic, dehydro- publication-title: Anal Biochem – volume: 824 start-page: 132 year: 2005 end-page: 138 ident: bb0125 article-title: High-throughput analysis of vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection publication-title: J Chromatogr B – volume: 500 start-page: 107 year: 2010 end-page: 115 ident: bb0150 article-title: Cellular pathways for transport and efflux of ascorbate and dehydroascorbate publication-title: Arch Biochem Biophys – volume: 877 start-page: 853 year: 2009 end-page: 856 ident: bb0165 article-title: Fast HPLC-ECD analysis of ascorbic acid, dehydroascorbic acid and uric acid publication-title: J Chromatogr B – volume: 21 start-page: 520 year: 2007 end-page: 526 ident: bb0095 article-title: Simultaneous determination of aminothiols, ascorbic acid and uric acid in biological samples by capillary electrophoresis with electrochemical detection publication-title: Biomed Chromatogr – volume: 27 start-page: 942 year: 2008 end-page: 958 ident: bb0140 article-title: HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids, TrAC publication-title: Trends Anal Chem – volume: 13 start-page: 129 year: 2006 end-page: 142 ident: bb0010 article-title: Oxidative stress and atherosclerosis publication-title: Pathophysiology – volume: 17 start-page: 409 year: 2007 end-page: 414 ident: bb0060 article-title: Uric acid and oxidative stress: relative impact on cardiovascular risk publication-title: Nutr Metab Cardiovasc Dis – volume: 407 start-page: 233 year: 2000 end-page: 241 ident: bb0005 article-title: Atherosclerosis publication-title: Nature – volume: 877 start-page: 2032 year: 2009 end-page: 2038 ident: bb0055 article-title: A sensitive and specific liquid chromatography–tandem mass spectrometry method for the determination of intracellular and extracellular uric acid publication-title: J Chromatogr B – volume: 281 start-page: 1415 year: 1999 end-page: 1423 ident: bb0030 article-title: Criteria and recommendations for vitamin C intake publication-title: JAMA – volume: 76 start-page: 1644 year: 2008 end-page: 1652 ident: bb0035 article-title: The controversial place of vitamin C in cancer treatment publication-title: Biochem Pharmacol – volume: 104 start-page: 419 year: 2009 end-page: 433 ident: bb0050 article-title: Role of marginal vitamin C deficiency in atherogenesis: in vivo models and clinical studies publication-title: Basic Clin Pharmacol Toxicol – volume: 42 start-page: 498 year: 1996 end-page: 506 ident: bb0015 article-title: Low-density lipoprotein oxidation, antioxidants, and atherosclerosis: a clinical biochemistry perspective publication-title: Clin Chem – volume: 97 start-page: 732 year: 2006 end-page: 736 ident: bb0115 article-title: Analysis of sugars, organic acids and vitamin C contents of blackberry genotypes from Turkey publication-title: Food Chem – volume: 61 start-page: 1233 year: 2007 end-page: 1236 ident: bb0105 article-title: Stability of whole blood and plasma ascorbic acid publication-title: Eur J Clin Nutr – volume: 689 start-page: 175 year: 1997 end-page: 180 ident: bb0130 article-title: Validation of chromatographic methods in biomedical analysis viewpoint and discussion publication-title: J Chromatogr B Biomed Sci Appl – volume: 34 start-page: 623 year: 2001 end-page: 627 ident: bb0135 article-title: Plasma ascorbic acid: measurement, stability and clinical utility revisited publication-title: Clin Biochem – volume: 11 start-page: 4145 year: 2005 end-page: 4151 ident: bb0065 article-title: Uric acid and oxidative stress publication-title: Curr Pharm Des – volume: 93 start-page: 707 year: 2000 end-page: 713 ident: bb0155 article-title: Uric acid as a risk factor for cardiovascular disease publication-title: QJM – volume: 107 start-page: 434 year: 2008 end-page: 438 ident: bb0120 article-title: Effect of pasteurisation on ascorbic acid, dehydroascorbic acid, tocopherols and fatty acids in pooled mature human milk publication-title: Food Chem – volume: 365 start-page: 249 year: 2006 end-page: 256 ident: bb0160 article-title: Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography publication-title: Clin Chim Acta – volume: 67 start-page: 95 year: 2006 end-page: 105 ident: bb0090 article-title: Pre-analytical factors affecting ascorbic and uric acid quantification in human plasma publication-title: J Biochem Biophys Methods – volume: 97 start-page: 732 year: 2006 ident: 10.1016/j.clinbiochem.2013.01.006_bb0115 article-title: Analysis of sugars, organic acids and vitamin C contents of blackberry genotypes from Turkey publication-title: Food Chem doi: 10.1016/j.foodchem.2005.09.023 – volume: 365 start-page: 249 year: 2006 ident: 10.1016/j.clinbiochem.2013.01.006_bb0160 article-title: Monitoring of antioxidant properties of uric acid in humans for a consideration measuring of levels of allantoin in plasma by liquid chromatography publication-title: Clin Chim Acta doi: 10.1016/j.cca.2005.09.002 – volume: 11 start-page: 4145 year: 2005 ident: 10.1016/j.clinbiochem.2013.01.006_bb0065 article-title: Uric acid and oxidative stress publication-title: Curr Pharm Des doi: 10.2174/138161205774913255 – volume: 877 start-page: 853 year: 2009 ident: 10.1016/j.clinbiochem.2013.01.006_bb0165 article-title: Fast HPLC-ECD analysis of ascorbic acid, dehydroascorbic acid and uric acid publication-title: J Chromatogr B doi: 10.1016/j.jchromb.2009.02.008 – volume: 137 start-page: 2171 year: 2007 ident: 10.1016/j.clinbiochem.2013.01.006_bb0045 article-title: New developments and novel therapeutic perspectives for vitamin C publication-title: J Nutr doi: 10.1093/jn/137.10.2171 – volume: 107 start-page: 434 year: 2008 ident: 10.1016/j.clinbiochem.2013.01.006_bb0120 article-title: Effect of pasteurisation on ascorbic acid, dehydroascorbic acid, tocopherols and fatty acids in pooled mature human milk publication-title: Food Chem doi: 10.1016/j.foodchem.2007.06.060 – volume: 407 start-page: 233 year: 2000 ident: 10.1016/j.clinbiochem.2013.01.006_bb0005 article-title: Atherosclerosis publication-title: Nature doi: 10.1038/35025203 – volume: 93 start-page: 707 year: 2000 ident: 10.1016/j.clinbiochem.2013.01.006_bb0155 article-title: Uric acid as a risk factor for cardiovascular disease publication-title: QJM doi: 10.1093/qjmed/93.11.707 – volume: 42 start-page: 498 year: 1996 ident: 10.1016/j.clinbiochem.2013.01.006_bb0015 article-title: Low-density lipoprotein oxidation, antioxidants, and atherosclerosis: a clinical biochemistry perspective publication-title: Clin Chem doi: 10.1093/clinchem/42.4.498 – volume: 61 start-page: 1233 year: 2007 ident: 10.1016/j.clinbiochem.2013.01.006_bb0105 article-title: Stability of whole blood and plasma ascorbic acid publication-title: Eur J Clin Nutr doi: 10.1038/sj.ejcn.1602655 – volume: 689 start-page: 175 year: 1997 ident: 10.1016/j.clinbiochem.2013.01.006_bb0130 article-title: Validation of chromatographic methods in biomedical analysis viewpoint and discussion publication-title: J Chromatogr B Biomed Sci Appl doi: 10.1016/S0378-4347(96)00297-6 – volume: 104 start-page: 419 year: 2009 ident: 10.1016/j.clinbiochem.2013.01.006_bb0050 article-title: Role of marginal vitamin C deficiency in atherogenesis: in vivo models and clinical studies publication-title: Basic Clin Pharmacol Toxicol doi: 10.1111/j.1742-7843.2009.00420.x – year: 1992 ident: 10.1016/j.clinbiochem.2013.01.006_bb0170 article-title: Ascorbic acid – volume: 500 start-page: 107 year: 2010 ident: 10.1016/j.clinbiochem.2013.01.006_bb0150 article-title: Cellular pathways for transport and efflux of ascorbate and dehydroascorbate publication-title: Arch Biochem Biophys doi: 10.1016/j.abb.2010.05.014 – volume: 21 start-page: 520 year: 2007 ident: 10.1016/j.clinbiochem.2013.01.006_bb0095 article-title: Simultaneous determination of aminothiols, ascorbic acid and uric acid in biological samples by capillary electrophoresis with electrochemical detection publication-title: Biomed Chromatogr doi: 10.1002/bmc.787 – volume: 34 start-page: 78 year: 1992 ident: 10.1016/j.clinbiochem.2013.01.006_bb0070 article-title: Two independent mutational events in the loss of urate oxidase during hominoid evolution publication-title: J Mol Evol doi: 10.1007/BF00163854 – volume: 281 start-page: 1415 year: 1999 ident: 10.1016/j.clinbiochem.2013.01.006_bb0030 article-title: Criteria and recommendations for vitamin C intake publication-title: JAMA doi: 10.1001/jama.281.15.1415 – volume: 54 start-page: 712 year: 1991 ident: 10.1016/j.clinbiochem.2013.01.006_bb0100 article-title: Ascorbic acid and dehydroascorbic acid measurements in human plasma and serum publication-title: Am J Clin Nutr doi: 10.1093/ajcn/54.4.712 – volume: 13 start-page: 129 year: 2006 ident: 10.1016/j.clinbiochem.2013.01.006_bb0010 article-title: Oxidative stress and atherosclerosis publication-title: Pathophysiology doi: 10.1016/j.pathophys.2006.05.002 – volume: 164 start-page: 353 year: 2001 ident: 10.1016/j.clinbiochem.2013.01.006_bb0040 article-title: New insights into the physiology and pharmacology of vitamin C publication-title: Can Med Assoc J – volume: 824 start-page: 132 year: 2005 ident: 10.1016/j.clinbiochem.2013.01.006_bb0125 article-title: High-throughput analysis of vitamin C in human plasma with the use of HPLC with monolithic column and UV-detection publication-title: J Chromatogr B doi: 10.1016/j.jchromb.2005.07.008 – volume: 4 start-page: B17 year: 2002 ident: 10.1016/j.clinbiochem.2013.01.006_bb0025 article-title: Antioxidants and atherosclerosis publication-title: Eur Heart J Supplements doi: 10.1016/S1520-765X(02)90011-8 – volume: 877 start-page: 2032 year: 2009 ident: 10.1016/j.clinbiochem.2013.01.006_bb0055 article-title: A sensitive and specific liquid chromatography–tandem mass spectrometry method for the determination of intracellular and extracellular uric acid publication-title: J Chromatogr B doi: 10.1016/j.jchromb.2009.05.037 – volume: 17 start-page: 409 year: 2007 ident: 10.1016/j.clinbiochem.2013.01.006_bb0060 article-title: Uric acid and oxidative stress: relative impact on cardiovascular risk publication-title: Nutr Metab Cardiovasc Dis doi: 10.1016/j.numecd.2007.02.011 – volume: 34 start-page: 623 year: 2001 ident: 10.1016/j.clinbiochem.2013.01.006_bb0135 article-title: Plasma ascorbic acid: measurement, stability and clinical utility revisited publication-title: Clin Biochem doi: 10.1016/S0009-9120(01)00270-3 – volume: 337 start-page: 408 year: 1997 ident: 10.1016/j.clinbiochem.2013.01.006_bb0020 article-title: Antioxidants and atherosclerotic heart disease publication-title: N Engl J Med doi: 10.1056/NEJM199708073370607 – volume: 27 start-page: 942 year: 2008 ident: 10.1016/j.clinbiochem.2013.01.006_bb0140 article-title: HPLC methods for simultaneous determination of ascorbic and dehydroascorbic acids, TrAC publication-title: Trends Anal Chem doi: 10.1016/j.trac.2008.08.006 – volume: 76 start-page: 1644 year: 2008 ident: 10.1016/j.clinbiochem.2013.01.006_bb0035 article-title: The controversial place of vitamin C in cancer treatment publication-title: Biochem Pharmacol doi: 10.1016/j.bcp.2008.09.024 – volume: 263 start-page: 176 year: 1998 ident: 10.1016/j.clinbiochem.2013.01.006_bb0085 article-title: Optimal conditions for the simultaneous ion-pairing HPLC determination ofl-ascorbic, dehydro-l-ascorbic, d-ascorbic, and uric acids with on-line ultraviolet absorbance and electrochemical detection publication-title: Anal Biochem doi: 10.1006/abio.1998.2773 – volume: 67 start-page: 95 year: 2006 ident: 10.1016/j.clinbiochem.2013.01.006_bb0090 article-title: Pre-analytical factors affecting ascorbic and uric acid quantification in human plasma publication-title: J Biochem Biophys Methods doi: 10.1016/j.jbbm.2006.01.006 – volume: 32 start-page: 66 year: 2012 ident: 10.1016/j.clinbiochem.2013.01.006_bb0110 article-title: Ascorbate and dehydroascorbic acid as biomarkers of oxidative stress: validity of clinical data depends on vacutainer system used publication-title: Nutr Res doi: 10.1016/j.nutres.2011.11.005 |
| SSID | ssj0002486 |
| Score | 2.2623005 |
| Snippet | Ascorbic (AA) and uric (UA) acids act as antioxidants and are capable to react with biologically relevant oxidants. We aimed to developed a simple, rapid,... |
| SourceID | proquest pubmed crossref elsevier |
| SourceType | Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 665 |
| SubjectTerms | Adult Antioxidants - analysis Ascorbic acid Ascorbic Acid - blood Atherosclerosis Chromatography, High Pressure Liquid - methods Female HPLC-UV Humans Ion-exclusion Male Middle Aged Plasma antioxidants Reproducibility of Results Ultraviolet Rays Uric acid Uric Acid - blood |
| Title | Rapid, sensitive and simultaneous determination of ascorbic and uric acids in human plasma by ion-exclusion HPLC-UV |
| URI | https://dx.doi.org/10.1016/j.clinbiochem.2013.01.006 https://www.ncbi.nlm.nih.gov/pubmed/23353131 https://www.proquest.com/docview/1327725248 |
| Volume | 46 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1ZaxRBEC5CAtEX0XhtjKEDPtpm-5jdachLWBLWI0HElX1r-oSROFkyG9AXf7tVcyQKCgFfhy6m6a-oo_urKoBXxmRtMGPjcZoc1zlm7nQouZfRGWd87q4uzs4n84V-tyyWGzAbamGIVtnb_s6mt9a6_3LYn-bhqqqoxhejGyHp6Y2c9pIq2PWUtPzNz1uah9TttEdazGn1Nhzccryo-tBXNJqKitKFajt40vCjv_uof8WgrS86fQgP-iCSHXf7fAQbqd6Be7NhdtsObJ_1T-aPofnkVlV8zRpiqpNtY66OrKmISejqhIk_iwMlhkBil5m5BnNSX4V2KbUdYi5UsWFVzdqZfmyFMfc3x_wPhhI8fQ8X13TrxuYfP8z44ssTWJyefJ7NeT9pgQdMP9bclFkGOckmqWBkmsZkpPDo21R0mVq8ORGj9AmDE-NELjFJSaEoZEZ7Ic1YqaewWV_W6TkwbcamjD5ljYFlMdF-mpNAyAtlvNFiPIJyOFsb-jbkNA3jwg58s6_2N1gswWLHwiIsI5A3oquuF8ddhI4GAO0fimXRZ9xF_GAA3SKG9JrSYWMxjcfMpED9GsGzThtudiWVQtumxO7__fwF3Jft9A3iV-7B5vrqOr3EGGjt91sl34et47fv5-e_AFqxB-8 |
| linkProvider | Elsevier |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1ZaxsxEB5SB5K-lDY94vRSoI8V8Uq79gr6EkzDprFNKXHxm9AJW5KN6TrQ_vvM7OG20EKgr4uGFfqGOaSZ-QDeKRVThRkb95NgeBp95CZ1ObfCG2WUje3VxXwxLpbpp1W22oFp3wtDZZWd7W9temOtuy8n3WmerMuSenwxukkEPb2R0149gF2aTpUNYPf0_KJYbA2ySBvCR1rPSWAPjn-VeVEDoi2JnYr60hPZDPEk_qO_u6l_haGNOzp7DI-6OJKdtlt9AjuhOoD9aU_fdgB78-7V_CnUX8y69O9ZTcXqZN6YqTyrSyomNFXA3J_5viqGcGI3kZka01JbumYpTR5ixpW-ZmXFGlo_tsaw-9ow-5OhBA8_3NUtXbyx4vNsypdfn8Hy7OPltOAd2QJ3mIFsuMqjcGIcVZBOiTDxQYnEonuT3kSa8mYS74UNGJ8ok8Qc85TgskxENBlCjaR8DoPqpgqHwFI1Urm3IaYYW2bj1E5iSBD1TCqr0mQ0hLw_W-26SeREiHGl-5Kzb_o3WDTBokeJRliGILai63Ycx32EPvQA6j90S6PbuI_4cQ-6RgzpQaXFRmMmj8lJhvo1hBetNmx3JaRE8yaTo__7-VvYLy7nMz07X1y8hIeiIeOgcstXMNh8vw2vMSTa2Dedyt8BXCcKoA |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Rapid%2C+sensitive+and+simultaneous+determination+of+ascorbic+and+uric+acids+in+human+plasma+by+ion-exclusion+HPLC-UV&rft.jtitle=Clinical+biochemistry&rft.au=Ferin%2C+Rita&rft.au=Pav%C3%A3o%2C+Maria+Leonor&rft.au=Baptista%2C+Jos%C3%A9&rft.date=2013-05-01&rft.issn=1873-2933&rft.eissn=1873-2933&rft.volume=46&rft.issue=7-8&rft.spage=665&rft_id=info:doi/10.1016%2Fj.clinbiochem.2013.01.006&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-9120&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-9120&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-9120&client=summon |