Multiple cancers escape from multiple MAPK pathway inhibitors and use DNA replication stress signaling to tolerate aberrant cell cycles
Many cancers harbor pro-proliferative mutations of the mitogen-activated protein kinase (MAPK) pathway. In BRAF-driven melanoma cells treated with BRAF inhibitors, subpopulations of cells escape drug-induced quiescence through a nongenetic manner of adaptation and resume slow proliferation. Here, we...
Saved in:
Published in | Science signaling Vol. 16; no. 796; p. eade8744 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.08.2023
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Many cancers harbor pro-proliferative mutations of the mitogen-activated protein kinase (MAPK) pathway. In BRAF-driven melanoma cells treated with BRAF inhibitors, subpopulations of cells escape drug-induced quiescence through a nongenetic manner of adaptation and resume slow proliferation. Here, we found that this phenomenon is common to many cancer types driven by EGFR, KRAS, or BRAF mutations in response to multiple, clinically approved MAPK pathway inhibitors. In 2D cultures and 3D spheroid models of various cancer cell lines, a subset of cells escaped drug-induced quiescence within 4 days to resume proliferation. These "escapee" cells exhibited DNA replication deficits, accumulated DNA lesions, and mounted a stress response that depended on the ataxia telangiectasia and RAD3-related (ATR) kinase. We further identified that components of the Fanconi anemia (FA) DNA repair pathway are recruited to sites of mitotic DNA synthesis (MiDAS) in escapee cells, enabling successful completion of cell division. Analysis of patient tumor samples and clinical data correlated disease progression with an increase in DNA replication stress response factors. Our findings suggest that many MAPK pathway-mutant cancers rapidly escape drug action and that suppressing early stress tolerance pathways may achieve more durable clinical responses to MAPK pathway inhibitors. |
---|---|
AbstractList | Many cancers harbor pro-proliferative mutations of the mitogen-activated protein kinase (MAPK) pathway. In BRAF-driven melanoma cells treated with BRAF inhibitors, subpopulations of cells escape drug-induced quiescence through a nongenetic manner of adaptation and resume slow proliferation. Here, we found that this phenomenon is common to many cancer types driven by EGFR, KRAS, or BRAF mutations in response to multiple, clinically approved MAPK pathway inhibitors. In 2D cultures and 3D spheroid models of various cancer cell lines, a subset of cells escaped drug-induced quiescence within 4 days to resume proliferation. These "escapee" cells exhibited DNA replication deficits, accumulated DNA lesions, and mounted a stress response that depended on the ataxia telangiectasia and RAD3-related (ATR) kinase. We further identified that components of the Fanconi anemia (FA) DNA repair pathway are recruited to sites of mitotic DNA synthesis (MiDAS) in escapee cells, enabling successful completion of cell division. Analysis of patient tumor samples and clinical data correlated disease progression with an increase in DNA replication stress response factors. Our findings suggest that many MAPK pathway-mutant cancers rapidly escape drug action and that suppressing early stress tolerance pathways may achieve more durable clinical responses to MAPK pathway inhibitors. |
Author | Yang, Chen Spencer, Sabrina L Armstrong, Claire Hoffman, Timothy E Ill, C Ryland Passanisi, Victor J Nangia, Varuna |
Author_xml | – sequence: 1 givenname: Timothy E orcidid: 0000-0001-8077-867X surname: Hoffman fullname: Hoffman, Timothy E organization: Department of Biochemistry and Biofrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA – sequence: 2 givenname: Varuna orcidid: 0000-0001-5142-4653 surname: Nangia fullname: Nangia, Varuna organization: Medical Scientist Training Program, University of Colorado-Anschutz Medical School, Aurora, CO 80045, USA – sequence: 3 givenname: C Ryland orcidid: 0000-0002-8224-2710 surname: Ill fullname: Ill, C Ryland organization: Department of Biochemistry and Biofrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA – sequence: 4 givenname: Victor J orcidid: 0000-0002-6961-9456 surname: Passanisi fullname: Passanisi, Victor J organization: Department of Biochemistry and Biofrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA – sequence: 5 givenname: Claire orcidid: 0000-0003-4387-2450 surname: Armstrong fullname: Armstrong, Claire organization: Department of Biochemistry and Biofrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA – sequence: 6 givenname: Chen surname: Yang fullname: Yang, Chen organization: Molecular Cellular and Developmental Biology, University of Colorado Boulder, Boulder, CO 80303, USA – sequence: 7 givenname: Sabrina L orcidid: 0000-0002-5798-3007 surname: Spencer fullname: Spencer, Sabrina L organization: Department of Biochemistry and Biofrontiers Institute, University of Colorado Boulder, Boulder, CO 80303, USA |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37527351$$D View this record in MEDLINE/PubMed |
BookMark | eNo1kNtKAzEYhIMo9qAP4I3kBbbmtJvsZalHbNULvS5J9t82ks0uSYr0CXxtC7UwMBfzMQwzQeehD4DQDSUzSll1l6xLbhO0n-kGlBTiDI1pzWVRU1GO0CSlb0Iqylh9iUZclkzyko7R72rnsxs8YKuDhZgwJKsHwG3sO9ydwtX84xUPOm9_9B67sHXG5f4A69DgXQJ8_zbHEQbvrM6uDzjlCCnh4yIXNjj3B3mIOgPWBmLUIWML3mO7tx7SFbpotU9w_e9T9PX48Ll4LpbvTy-L-bKwgqlcKC6IrTixRnJOFNOCGmGEqqxSpeW8rWppOGdlbUrScGEJKSWhqpWCS0ksm6LbY--wMx006yG6Tsf9-vQI-wNdWmZa |
CitedBy_id | crossref_primary_10_1002_mc_23684 crossref_primary_10_1073_pnas_2321216121 crossref_primary_10_1038_s41540_024_00369_x |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1126/scisignal.ade8744 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Anatomy & Physiology |
EISSN | 1937-9145 |
ExternalDocumentID | 37527351 |
Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GrantInformation_xml | – fundername: NCI NIH HHS grantid: T32 CA174648 – fundername: NIH HHS grantid: S10 OD021601 – fundername: NIH HHS grantid: S10 OD025072 |
GroupedDBID | --- 0R~ 123 18M 4.4 53G 7~K ABJNI ACGFO ACGFS ACIWK ACPRK AENEX AFQFN AFRAH AJGZS ALMA_UNASSIGNED_HOLDINGS BKF BYM CGR CS3 CUY CVF DU5 EBS ECM EIF EJD EMOBN F5P HZ~ NPM O9- P2P RHF RHI SJN |
ID | FETCH-LOGICAL-c428t-8340c630cb733082a41b4b486c885c33f697b33259b50d34c0057018f743770c2 |
IngestDate | Tue Aug 27 13:51:14 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 796 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c428t-8340c630cb733082a41b4b486c885c33f697b33259b50d34c0057018f743770c2 |
ORCID | 0000-0001-5142-4653 0000-0002-5798-3007 0000-0001-8077-867X 0000-0002-8224-2710 0000-0003-4387-2450 0000-0002-6961-9456 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10055235 |
PMID | 37527351 |
ParticipantIDs | pubmed_primary_37527351 |
PublicationCentury | 2000 |
PublicationDate | 2023-08-01 |
PublicationDateYYYYMMDD | 2023-08-01 |
PublicationDate_xml | – month: 08 year: 2023 text: 2023-08-01 day: 01 |
PublicationDecade | 2020 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Science signaling |
PublicationTitleAlternate | Sci Signal |
PublicationYear | 2023 |
References | 36993538 - bioRxiv. 2023 Mar 21 |
References_xml | |
SSID | ssj0061229 |
Score | 2.4651928 |
Snippet | Many cancers harbor pro-proliferative mutations of the mitogen-activated protein kinase (MAPK) pathway. In BRAF-driven melanoma cells treated with BRAF... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | eade8744 |
SubjectTerms | Cell Cycle Cell Cycle Proteins - metabolism Cell Line, Tumor DNA Replication Humans MAP Kinase Signaling System - genetics Mutation Neoplasms - drug therapy Neoplasms - genetics Protein Kinase Inhibitors - pharmacology Protein Kinase Inhibitors - therapeutic use Proto-Oncogene Proteins B-raf - genetics Proto-Oncogene Proteins B-raf - metabolism |
Title | Multiple cancers escape from multiple MAPK pathway inhibitors and use DNA replication stress signaling to tolerate aberrant cell cycles |
URI | https://www.ncbi.nlm.nih.gov/pubmed/37527351 |
Volume | 16 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1Lb9NAEIBXKUioFwSU90NzQFwsV47X8a6PEQ8VUCIObdVbtbtZ00jUrmJHKPwBfhH_jxnvrmOqgICLFdlx5Hg-zc57GXvJ0UeWUutYZZmNs1wlMY2ti1MlLJcLbuyE-p1n8_zoJPtwNjkbjX4MqpbWrT4033b2lfyPVPEcypW6ZP9Bsv2P4gn8jPLFI0oYj38l41moBjQku1UT2YbqmVzPSF8qOJt--kjzUy--Kmryu1jqZbfFDoXM142N3synlDoI0bvQPkKVHaprVifztP5C05dtpLRd4fLWRhTxj8yGiuqGBm7QFf3dPTd1Wfpoq-dj2wSBKv6zK9o9Vat11a8U711OBCHY-J1GfLqraVS1bLpChNMlpR18dsuHL1LeF8_h6uNULhpIqHLdUMleJ-cD9kQxVLFU4U0z-3fr_7BjpfuXhzu-iyK8uuyA4IKmz7l5t3--em0kd7i0x_aEJLU6pxCRW_7RYkwLnz733Vm_Pss-uxXuv-bKdCbN8R122_siMHVg3WUjW91jB9NKtfXlBl5BVx3cpV0O2PfAGnjWwLEGxBoE1oBYA88abFkDlB4ga4CswYA1cKxBTwu0NQTWILAGxBo41u6zk3dvj18fxX4Tj9igZ9vGkmeJyXlitCC1kKpsrDOdydxIOTGcl3khNOfohetJsuCZofboZCxLNG2FSEz6gN2o6so-YpCYolRlWugF5xm6utKYcaLyQlshJDoCj9lD9zrPr9yklvPwop_89spTtr-l8hm7WaJqsM_Rzmz1i06uPwESUIRp |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Multiple+cancers+escape+from+multiple+MAPK+pathway+inhibitors+and+use+DNA+replication+stress+signaling+to+tolerate+aberrant+cell+cycles&rft.jtitle=Science+signaling&rft.au=Hoffman%2C+Timothy+E&rft.au=Nangia%2C+Varuna&rft.au=Ill%2C+C+Ryland&rft.au=Passanisi%2C+Victor+J&rft.date=2023-08-01&rft.eissn=1937-9145&rft.volume=16&rft.issue=796&rft.spage=eade8744&rft_id=info:doi/10.1126%2Fscisignal.ade8744&rft_id=info%3Apmid%2F37527351&rft_id=info%3Apmid%2F37527351&rft.externalDocID=37527351 |