Evaluation of fungal capacity for detoxification of extractives in Scots pine sapwood

• Published in: Environmental technology Vol. 21; no. 5; pp. 569 - 575
• Main Authors: Martinez-Inigo, M.J, Claassen, F.W, Joseleau, B, Beek, T.A. van, Lenon, G, Sierra-Alvarez, R
• Format: Journal Article
• Language: English
• Published: London Taylor & Francis Group 01.05.2000; Selper
• Subjects: ALBURA; Applied sciences; AUBIER; BIODEGRADACION; BIODEGRADATION; Biological and medical sciences; Biotechnology; CORIOLUS VERSICOLOR; DECAY FUNGI; DESINTOXICACION; DETOXIFICATION; EPS; Exact sciences and technology; EXTRACTIVES; EXTRACTOS VEGETALES; EXTRAIT D'ORIGINE VEGETALE; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Laboratorium voor Organische chemie; Laboratory for Organic Chemistry; Organic Chemistry; Organische Chemie; Other applications; Paper, paperboard, non wovens; PINUS SYLVESTRIS; PLANT EXTRACTS; Polymer industry, paints, wood; Pulp manufacturing; sapstain; SAPWOOD; STAIN FUNGI; STEREUM SANGUINOLENTUM; TOXICIDAD; TOXICITE; TOXICITY; white-rot fungi; WOOD DESTROYING FUNGI; Wood extractives; Wood. Paper. Non wovens; Biodegradation; Sapwood; Bioassay; Toxicity; Paper pulp; Softwood; Extract; Raw materials; Experimental study; Pinus sylvestris; Fatty acids; Fungi; Wood chip; Gymnospermae; Coniferales; Spermatophyta; Manufacturing; Resinic acid; Thallophyta; Acetone
• ISSN: 0959-3330; 1479-487X
• DOI: 10.1080/09593332408618094

Ninety wood-inhabiting fungi were screened for their ability to degrade and detoxify acetone extractives in Scots pine (Pinus sylvestris L.) sapwood under solid-state fermentation conditions. Fungal degradation of resin acids and long-chain fatty acids was investigated by high pressure liquid chromatography with evaporative light scattering detection (HPLC-ELSD) and the toxicity of the acetone soluble fraction of wood measured by Microtox assay. Discriminant analysis of data showed different patterns of extractive detoxification for white-rot basidiomycetes and sapstain fungi. The major detoxification skills of basidiomycetes appeared to be related to the highest degradation of resin acids, and particularly dehydroabietic acid. Among them, Ischnoderma benzoinum, Stereum sanguinolentum and Trametes versicolor greatly reduced the sapwood toxicity due to acetone extractives from the EC 50 value of 0.08 mg wood ml −1 to levels between 5-10 mg wood ml −1 .