Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy

• Published in: Nanoscale Vol. 13; no. 15; pp. 7285 - 7293
• Main Authors: Casasanta, Michael A, Jonaid, G. M, Kaylor, Liam, Luqiu, William Y, Solares, Maria J, Schroen, Mariah L, Dearnaley, William J, Wilson, Jarad, Dukes, Madeline J, Kelly, Deborah F
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 21.04.2021
• Subjects: Algorithms; Antibodies; Binding sites; Coronavirus Nucleocapsid Proteins - chemistry; Cryoelectron Microscopy; Macromolecules; Microscopy; Molecular structure; Molecular Weight; Pandemics; Phosphoproteins - chemistry; Protein Structure, Secondary; Proteins; SARS-CoV-2 - chemistry; Semiconductors; Severe acute respiratory syndrome coronavirus 2; Transmission electron microscopy; Viral diseases

Interest in cryo-Electron Microscopy (EM) imaging has skyrocketed in recent years due to its pristine views of macromolecules and materials. As advances in instrumentation and computing algorithms spurred this progress, there is renewed focus to address specimen-related challenges. Here we contribut...