Engineering enzyme activity using an expanded amino acid alphabet

• Published in: Protein engineering, design and selection Vol. 36
• Main Authors: Birch-Price, Zachary, Taylor, Christopher J, Ortmayer, Mary, Green, Anthony P
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 21.01.2023
• Subjects: Amino Acids - chemistry; Amino Acids - genetics; Biocatalysis; Cloning, Molecular; Genetic Code - genetics; Proteins - chemistry; Review; non-canonical amino acids; genetic code expansion; directed evolution

Abstract Enzyme design and engineering strategies are typically constrained by the limited size of nature’s genetic alphabet, comprised of only 20 canonical amino acids. In recent years, site-selective incorporation of non-canonical amino acids (ncAAs) via an expanded genetic code has emerged as a p...