Small interfering RNA molecules as potential anti-human rhinovirus agents: in vitro potency, specificity, and mechanism

• Published in: Antiviral research Vol. 61; no. 1; pp. 49 - 55
• Main Authors: Phipps, Krista M., Martinez, Alejandro, Lu, Jin, Heinz, Beverly A., Zhao, Genshi
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2004; Elsevier
• Subjects: Anti-viral agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Antiviral Agents - pharmacology; Base Sequence; Biological and medical sciences; Dose-Response Relationship, Drug; Genome, Viral; HeLa Cells; Human rhinovirus 16; Humans; In Vitro Techniques; Medical sciences; Pharmacology. Drug treatments; Rhinovirus; Rhinovirus - drug effects; Rhinovirus - genetics; Rhinovirus - growth & development; RNA Interference; RNA silencing; RNA, Double-Stranded - pharmacology; RNA, Small Interfering - pharmacology; RNA, Viral - metabolism; Sensitivity and Specificity; siRNA; Transfection; Virus Replication - drug effects; siRNA; RNA silencing; Anti-viral agents; Rhinovirus; RNA; Picornaviridae; In vitro; Human rhinovirus; Virus; Gene silencing; Antiviral; Replication; Inhibition; Genome; Mechanism of action

RNA silencing or interference (RNAi) is a sequence-specific, post-transcriptional process of mRNA degradation. The degradation of target gene mRNA can be induced by short dsRNA molecules (21–25-nt) corresponding to the sequence of the target gene to be silenced. Short dsRNA molecules have been shown...