Widely Distributed Bifunctional Bacterial Cytochrome P450 Enzymes Catalyze both Intramolecular C−C Bond Formation in cyclo‐l‐Tyr‐l‐Tyr and Its Coupling with Nucleobases

• Published in: Angewandte Chemie International Edition Vol. 61; no. 21; pp. e202200377 - n/a
• Main Authors: Liu, Jing, Harken, Lauritz, Yang, Yiling, Xie, Xiulan, Li, Shu‐Ming
• Format: Journal Article
• Language: English
• Published: WEINHEIM Wiley 16.05.2022; Wiley Subscription Services, Inc; John Wiley and Sons Inc
• Edition: International ed. in English
• Subjects: Adducts; Bases (nucleic acids); Biocatalysts; Biosynthesis; Biosynthetic Pathways; Catalysis; Chemistry; Chemistry, Multidisciplinary; Coupling; Cyclodipeptide Synthase; Cytochrome; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Cytochrome P450 Enzymes; Cytochromes P450; E coli; Enzymes; Escherichia coli - genetics; Escherichia coli - metabolism; Gene clusters; Guanine; Hypoxanthine; Natural Products; Nucleobase Transferase; Phylogeny; Physical Sciences; Science & Technology; Transferases - metabolism; Tryptophan; Tyrosine; Nucleobase Transferase; Natural Products; GENE-CLUSTER; PATHWAY; Cytochrome P450 Enzymes; CYP121; Biosynthesis; DIVERSITY; IDENTIFICATION; Cyclodipeptide Synthase

Tailoring enzymes are important modification biocatalysts in natural product biosynthesis. We report herein six orthologous two‐gene clusters for mycocyclosin and guatyromycine biosynthesis. Expression of the cyclodipeptide synthase genes gymA1–gymA6 in Escherichia coli resulted in the formation of...