Analysis of mRNA nuclear export kinetics in mammalian cells by microinjection

• Published in: Journal of visualized experiments no. 46
• Main Authors: Gueroussov, Serge, Tarnawsky, Stefan P, Cui, Xianying A, Mahadevan, Kohila, Palazzo, Alexander F
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 04.12.2010
• Subjects: Active Transport, Cell Nucleus - physiology; Animals; Cell Line; Cellular Biology; Cercopithecus aethiops; COS Cells; DNA - genetics; In Situ Hybridization, Fluorescence - methods; Kinetics; Mice; Microinjections - instrumentation; Microinjections - methods; NIH 3T3 Cells; Plasmids - genetics; RNA, Messenger - analysis; RNA, Messenger - metabolism; Subcellular Fractions - chemistry; Subcellular Fractions - metabolism

In eukaryotes, messenger RNA (mRNA) is transcribed in the nucleus and must be exported into the cytoplasm to access the translation machinery. Although the nuclear export of mRNA has been studied extensively in Xenopus oocytes and genetically tractable organisms such as yeast and the Drosophila deri...