Molecular cloning and characterization of a sym plasmid locus that regulates cultivar-specific nodulation of soybean by Rhizobium fredii USDA257

• Published in: Molecular microbiology Vol. 9; no. 1; p. 17
• Main Authors: Meinhardt, L W, Krishnan, H B, Balatti, P A, Pueppke, S G
• Format: Journal Article
• Language: English
• Published: England 01.07.1993
• Subjects: Amino Acid Sequence; Base Sequence; Cosmids - genetics; DNA, Bacterial - genetics; Flavonoids - pharmacology; Gene Expression Regulation, Bacterial - drug effects; Genes, Bacterial; Glycine max - classification; Glycine max - microbiology; Molecular Sequence Data; Mutagenesis, Insertional; Open Reading Frames; Phenotype; Recombinant Fusion Proteins - biosynthesis; Rhizobium - genetics; Rhizobium - physiology; Sequence Homology, Amino Acid; Species Specificity; Symbiosis - genetics

Rhizobium fredii strain USDA257 produces nitrogen-fixing nodules on primitive soybean cultivars such as Peking but fails to nodulate agronomically improved cultivars such as McCall. Transposon-mutant 257DH4 has two new phenotypes: it nodulates McCall, and its ability to do so is sensitive to the pre...