STUDIES ON A NEW IMMUNOACTIVE PEPTIDE, FK-156 II. FERMENTATION, EXTRACTION AND CHEMICAL AND BIOLOGICAL CHARACTERIZATION

• Published in: Journal of antibiotics Vol. 35; no. 10; pp. 1286 - 1292
• Main Authors: GOTOH, TOSHIO, NAKAHARA, KUNIO, NISHIURA, TOYOJI, HASHIMOTO, MASAHARU, KINO, TORU, KURODA, YOSHIO, OKUHARA, MASAKUNI, KOHSAKA, MASANOBU, AOKI, HATSUO, IMANAKA, HIROSHI
• Format: Journal Article
• Language: English
• Published: Japan JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1982
• Subjects: Adjuvants, Immunologic - isolation & purification; Amino Acids, Diamino - isolation & purification; Animals; Antibody Formation - drug effects; Antineoplastic Agents; biological properties; Carbohydrate Metabolism; Chemical Phenomena; Chemistry; Chemistry, Physical; Diaminopimelic Acid - analogs & derivatives; Diaminopimelic Acid - isolation & purification; Diaminopimelic Acid - pharmacology; Escherichia coli Infections - prevention & control; Female; Fermentation; Guinea Pigs; Hypersensitivity, Delayed - prevention & control; immunostimulants; Male; Mice; Mice, Inbred BALB C; Mitogens; peptides; Rats; Streptomyces
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.35.1286

An interesting immunoactive peptide, FK-156, has been isolated from the fermentation broth of Streptomyces olivaceogriseus sp. nov. and Streptomyces violaceus. The compound was purified by column chromatography with activated carbon, ion exchange Sephadex and cellulose powder. FK-156, obtained as white powder, exhibits a wide variety of immunostimulatory activity in vivo and in vitro with experimental animals. Pretreatment of mice or rats with this peptide protected the animals against lethal infection with Escherichia coli and resulted in prolongation of life span of tumor bearing animals.