The effect of cell density on the maturation and contractile ability of muscle derived cells in a 3D tissue-engineered skeletal muscle model and determination of the cellular and mechanical stimuli required for the synthesis of a postural phenotype

• Published in: Journal of cellular physiology Vol. 225; no. 3; pp. 646 - 653
• Main Authors: Mudera, V., Smith, A.S.T., Brady, M.A., Lewis, M.P.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.12.2010
• Subjects: Adult; Biomimetics; Cell Count; Cell Culture Techniques; Cell Differentiation - genetics; Collagen - metabolism; Gene Expression Regulation; Humans; Mechanotransduction, Cellular; Muscle Cells - physiology; Muscle Development - genetics; Muscle Strength; Muscle, Skeletal - cytology; Muscle, Skeletal - physiology; Phenotype; RNA, Messenger - metabolism; Time Factors; Tissue Engineering - methods; Young Adult

The successful engineering of a truly biomimetic model of skeletal muscle could have a significant impact on a number of biomedical disciplines. Although a variety of techniques are currently being developed, there is, as of yet, no widely available and easily reproducible culture system for the syn...