Relationship between periodontitis-associated subgingival microbiota and clinical inflammation by 16S pyrosequencing

Aim To analyse the relationship between the chronic periodontitis‐associated subgingival microbiota and clinical inflammation. Material and Methods Sixty subjects with generalized chronic periodontitis participated in this study. Patients were divided into two groups according to their bleeding on p...

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Published inJournal of clinical periodontology Vol. 42; no. 12; pp. 1074 - 1082
Main Authors Camelo-Castillo, A, Novoa, L, Balsa-Castro, C, Blanco, J, Mira, A, Tomás, I
Format Journal Article
LanguageEnglish
Published United States Blackwell Publishing Ltd 01.12.2015
Subjects
16S ribosomal RNA
bacterial load
Bacteroides
Capnocytophaga
chronic periodontitis
Dental Plaque
Desulfobulbus
Eubacterium
Genes
Humans
Inflammation
microbiome
Microbiota
Periodontitis
Porphyromonas gingivalis
pyrosequencing
RNA, Ribosomal, 16S
Streptococcus constellatus
subgingival plaque
Treponema
Veillonella
pyrosequencing
bacterial load
subgingival plaque
16S ribosomal RNA
microbiome
chronic periodontitis
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Summary:Aim To analyse the relationship between the chronic periodontitis‐associated subgingival microbiota and clinical inflammation. Material and Methods Sixty subjects with generalized chronic periodontitis participated in this study. Patients were divided into two groups according to their bleeding on probing (BOP) scores: BOP‐1 group (mean scores ≤50% in sampled sites) and BOP‐2 group (mean scores >50%). Subgingival bacterial samples from periodontal patients were studied by pyrosequencing PCR products of the 16S rRNA gene and by real‐time PCR. Results In all the analysed subgingival samples, 102 bacterial genera and 203 species (from 41 genera of interest) were identified. Rarefaction curves showed a greater number of bacterial species in samples from BOP‐2 group compared to BOP‐1 group. The BOP‐1 group had significantly higher abundance percentages of Anaeroglobus (especifically, A. geminatus), Capnocytophaga (especifically C. gingivalis), TM7 and Veillonella. The BOP‐2 had significantly higher abundance percentages of Desulfobulbus (especially D. propionicus), Eubacterium (especially E. saphenum), Filifactor alocis, Streptococcus constellatus, Tannerella (especially, T. forsythia) and Treponema. Conclusion 16S pyrosequencing revealed that increased inflammation, at sites with periodontitis, is associated with a more diverse subgingival microbiota and specific changes in the bacterial composition, involving “established” periopathogens, symbionts and novel low‐abundance pathobionts.
Bibliography:ark:/67375/WNG-XZL6GZ4X-4
istex:F65E9A7CDA399BF682D287C0C18C22063325F149
Regional Ministry of Culture, Education and University - No. EM2014/025
Fig. S1. Mean relative abundance of bacterial taxa in the periodontal patients.Table S1. Mean relative abundance at the genus level, with standard deviation (in parentheses), distinguishing between BOP-1 and BOP-2 groups.Table S2. Mean relative abundance at the species level, with standard deviation (in parentheses), distinguishing between BOP-1 and BOP-2 groups.
National Plan of Research, Development and Innovation
ArticleID:JCPE12470
Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain) - No. PI11/01383
European Regional Development Fund
This work was supported by project PI11/01383 from Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain), which is integrated in National Plan of Research, Development and Innovation (PN I + D + I 2008–2011). This project was co‐financed by European Regional Development Fund (ERDF 2007–2013).
This work was also funded by project EM2014/025 from Regional Ministry of Culture, Education and University (regional government of Galicia, Spain), which is integrated in Regional Plan of Research, Innovation and Development 2011‐2015.
Conflict of interest and source of funding statement
The authors declare that they have no conflict of interests. The funding institutions had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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ISSN:0303-6979
1600-051X
1600-051X
DOI:10.1111/jcpe.12470