Soluble form of LR11 is highly increased in the vitreous fluids of patients with idiopathic epiretinal membrane

• Published in: Graefe's archive for clinical and experimental ophthalmology Vol. 255; no. 5; pp. 885 - 891
• Main Authors: Hashimoto, Ryuya, Jiang, Meizi, Shiba, Tomoaki, Hiruta, Nobuyuki, Takahashi, Mao, Higashi, Morihiro, Hori, Yuichi, Bujo, Hideaki, Maeno, Takatoshi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2017; Springer Nature B.V
• Subjects: Aged; Biomarkers - metabolism; Cell Movement; Enzyme-Linked Immunosorbent Assay; Epiretinal Membrane - metabolism; Epiretinal Membrane - pathology; Female; Humans; LDL-Receptor Related Proteins - metabolism; Male; Medicine; Medicine & Public Health; Membrane Transport Proteins - metabolism; Nerve Tissue Proteins; Ophthalmology; Retinal Disorders; Retrospective Studies; Transforming Growth Factor beta2 - metabolism; Vitreous Body - metabolism; Vitreous Body - pathology; Soluble LR11; Transforming growth factorβ2; Vitreous surgery; Idiopathic epiretinal membrane; Vitreous fluids
• DOI: 10.1007/s00417-017-3585-1
• ISSN: 0721-832X; 1435-702X

Purpose LR11 (also called SorLA or SORL1) is a migration regulator of adherent cells with the immature proliferative phenotype. The present study investigated the clinical and pathological involvement of the soluble form of LR11 (sLR11) in the idiopathic epiretinal membrane (iERM). Methods The subjects were 51 patients with iERM (24 cellophane macular reflex (CMR) and 27 preretinal macular fibrosis (PMF)) and 45 patients with macular holes as age and sex-matched controls. Vitreous sLR11 and transforming growth factor (TGF)β2 levels were measured by ELISA. Results The sLR11 levels in the vitreous fluids of patients with iERM (20.2 ± 8.1 ng/mL) were significantly higher than those in controls (11.4 ± 4.7 ng/mL). Among the patients with iERM, the vitreous sLR11 levels were significantly higher in PMF (23.6 ± 8.2 ng/mL), than those in CMR (16.5 ± 5.9 ng/mL). Multivariate regression analysis of the studied factors showed that sLR11 was a unique factor independently contributing to the discrimination of the iERM patients against the control subjects (odds ratio [OR] 1.35 per 1-ng/mL increase, 95% CI 1.09–1.67; P  = 0.004). ROC analysis showed that the sensitivity and the specificity of sLR11, but not of other studied factors, categorized into the rank of moderate accuracy. Finally, there was a positive correlation (R = 0.588; P  = 0.003) between the vitreous levels of sLR11 and TGFβ2 using the available samples. Conclusions sLR11 levels in vitreous fluids were specifically increased in patients with iERM, suggesting the involvement in the pathology of proliferative and migrating cells for the development of iERM.