Intracytoplasmic injection of frozen-thawed epididymal spermatozoa in a nonhuman primate model, the cynomolgus monkey ( Macaca fascicularis)

• Published in: Theriogenology Vol. 58; no. 7; pp. 1385 - 1397
• Main Authors: Ng, Soon Chye, Martelli, Paolo, Liow, Swee Lian, Herbert, Sasha, Oh, Soon Hock
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.10.2002
• Subjects: Animals; Animals, Newborn; Cryopreservation - methods; Cryopreservation - veterinary; Embryo Transfer - veterinary; Epididymal sperm; Epididymis - physiology; Female; Gonadotropin; ICSI; Macaca fascicularis - physiology; Male; Monkey; Oocyte; Oocytes - physiology; Ovulation Induction - veterinary; Pregnancy; Semen Preservation - methods; Semen Preservation - veterinary; Singapore; Sperm Injections, Intracytoplasmic - methods; Sperm Injections, Intracytoplasmic - veterinary; Spermatozoa - physiology; Singapore; ICSI; Epididymal sperm; Gonadotropin; Oocyte; Monkey

Intracytoplasmic sperm injection (ICSI) with frozen-thawed epididymal spermatozoa was performed in the cynomolgus monkey ( Macaca fascicularis) to produce embryos in vitro. Eleven sexually mature females were hyperstimulated with an GnRH agonist (1.8 mg active triptorelin per 2 kg body weight), foll...