A modular atomic force microscopy approach reveals a large range of hydrophobic adhesion forces among bacterial members of the leaf microbiota

• Published in: The ISME Journal Vol. 13; no. 7; pp. 1878 - 1882
• Main Authors: Mittelviefhaus, Maximilian, Müller, Daniel B, Zambelli, Tomaso, Vorholt, Julia A
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.07.2019; Nature Publishing Group UK
• Subjects: Adhesion; Adhesion tests; Arabidopsis - microbiology; Atomic force microscopy; Bacteria; Bacterial Adhesion; Beads; Brief Communication; Colonization; Gammaproteobacteria - genetics; Gammaproteobacteria - isolation & purification; Gammaproteobacteria - physiology; Hydrophobic and Hydrophilic Interactions; Hydrophobicity; Immobilization; Leaves; Microbiota; Microbiota - genetics; Microbiota - physiology; Microscopes; Microscopy; Microscopy, Atomic Force - methods; Morphology; Outbreaks; Phylogeny; Plant Leaves - microbiology; Surface Properties

Bacterial adhesion is the initial step in surface colonization and community formation. At the single-cell level, atomic force microscopy (AFM) techniques have enabled the quantification of adhesive forces between bacteria and substrata. However, conventional techniques depend on the irreversible im...