Generating favorable growth factor and protease release profiles to enable extracellular matrix accumulation within an in vitro tissue engineering environment

• Published in: Acta biomaterialia Vol. 54; pp. 81 - 94
• Main Authors: Zhang, Xiaoqing, Battiston, Kyle G., Labow, Rosalind S., Simmons, Craig A., Santerre, J. Paul
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2017; Elsevier BV
• Subjects: Accumulation; Bioaccumulation; Biomaterial; Cell culture; Co-culture; Coculture Techniques; Collagen; Collagenase 3; Collagenases - secretion; Connective tissues; Construction engineering; Cytokines; Cytokines - secretion; Degradation; Deposition; Elastin; Electrochemical machining; Extracellular matrix; Extracellular Matrix - chemistry; Gelatinase A; Gelatinase B; Growth factors; Humans; Immune system; In vitro methods and tests; In vivo methods and tests; Insulin; Insulin-like growth factor I; Matrix metalloproteinase; Monocytes; Monocytes - cytology; Monocytes - secretion; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - secretion; Myocytes, Smooth Muscle - cytology; Myocytes, Smooth Muscle - secretion; Polyurethane; Polyurethane resins; Protease; Proteinase; Regeneration; Scaffolds; Signaling; Smooth muscle; Studies; Tissue culture; Tissue Engineering; Underlying mechanisms; Vascular tissue; Extracellular matrix; Underlying mechanisms; Biomaterial; Matrix metalloproteinase; Co-culture

[Display omitted] Tissue engineering (particularly for the case of load-bearing cardiovascular and connective tissues) requires the ability to promote the production and accumulation of extracellular matrix (ECM) components (e.g., collagen, glycosaminoglycan and elastin). Although different approach...