Protective effect of metallothionein to ras DNA damage induced by hydrogen peroxide and ferric ion-nitrilotriacetic acid

• Published in: Chemico-biological interactions Vol. 122; no. 3; pp. 137 - 152
• Main Authors: Min, Kyong-Son, Nishida, Kayo, Onosaka, Satomi
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.11.1999
• Subjects: Animals; Cadmium - pharmacology; Deoxyribose - metabolism; DNA Damage - drug effects; DNA strand scission; DNA, Single-Stranded - drug effects; Edetic Acid - pharmacology; Ethylmaleimide - pharmacology; Ferric Compounds - antagonists & inhibitors; Ferric Compounds - toxicity; Genes, ras - genetics; Glutathione - pharmacology; Humans; Hydrogen Peroxide - antagonists & inhibitors; Hydrogen Peroxide - toxicity; Hydroxyl radical; Male; Metallothionein; Metallothionein - pharmacology; Mice; Nitrilotriacetic Acid - analogs & derivatives; Nitrilotriacetic Acid - antagonists & inhibitors; Nitrilotriacetic Acid - toxicity; Protective effect; Zinc - pharmacology; NTA, nitrilotriacetic acid; GSH, glutathione; Protective effect; O 2 −, superoxide anion; EDTA, ethylenediamine- N, N, N′, N′-tetraacetic acid; OH, hydroxyl radical; DTPA, diethylenetriaminepentaacetic acid; MT, metallothionein; CA, citric acid; DNA strand scission; CDTA, trans-1,2-diaminocyclohexane- N, N, N′, N′-tetraacetic acid; Metallothionein; Hydroxyl radical

Metallothionein (MT) is a strong antioxidant, due to a large number of thiol groups in the MT molecule and MT has been found in the nucleus. To investigate whether MT can directly protect DNA from damage induced by hydroxyl radical, the effects of MTs on DNA strand scission due to incubation with fe...