Cloning and molecular characterization of Dashurin encoded by C20orf116, a PCI-domain containing protein

• Published in: Biochimica et biophysica acta Vol. 1800; no. 4; pp. 430 - 438
• Main Authors: Neziri, D., Ilhan, A., Maj, M., Majdic, O., Baumgartner-Parzer, S., Cohen, G., Base, W., Wagner, L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2010
• Subjects: C20orf116; Carcinoma, Hepatocellular - genetics; Cell Line, Tumor; Chromosome Mapping; Chromosomes, Human, Pair 20 - genetics; Cloning, Molecular - methods; Dashurin; Genome, Human; GFP; Green Fluorescent Proteins - genetics; Humans; Kidney - physiology; Leukemia, Myeloid, Acute - genetics; Liver Neoplasms - genetics; Luciferases - genetics; Open Reading Frames; PCI-domain; Proteasome Endopeptidase Complex - genetics; Protein Biosynthesis; Proteins - genetics; Recombinant Fusion Proteins - genetics; RNA, Messenger - genetics; Transcription; Transcription, Genetic; Transfection; Translation; Translocation, Genetic; SH-SY5Y; RT; Transcription; PCI-domain; HRP; Dashurin; GFP; HEK293; PPTK; Xgal; HepG2; RLU; eIF3; CSN; PBS; Translation; TSA; DAwoSP; GST; TPBS; mAb; SDS-PAGE; LB; PCI; IPTG; C20orf116; Ni-NTA; PCR; TRIC

Characterization of gene products originating from undefined open reading frames and delineation of biological functions has become the task after the human genome has been decoded. We cloned the human C20orf 116 and defined its transcript in liver, kidney and various brain regions by Northern analy...