A multiplex DNA suspension microarray for simultaneous detection and differentiation of classical swine fever virus and other pestiviruses

• Published in: Journal of virological methods Vol. 136; no. 1; pp. 17 - 23
• Main Authors: Deregt, Dirk, Gilbert, Scott A., Dudas, Sandor, Pasick, John, Baxi, Shailja, Burton, Kimberley M., Baxi, Mohit K.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.09.2006; Amsterdam Elsevier; New York, NY
• Subjects: 5' Untranslated Regions - genetics; Biological and medical sciences; Biotin; Border disease virus; Bovine viral diarrhea virus; Classical swine fever virus; DNA suspension microarray; Fluorescence; Fundamental and applied biological sciences. Psychology; Genotype; Microbiology; Microspheres; Oligonucleotide Array Sequence Analysis - methods; Pestivirus; Pestivirus - classification; Pestivirus - genetics; Polymerase Chain Reaction - methods; RNA, Viral - genetics; Sensitivity and Specificity; Staining and Labeling; Streptavidin; Techniques used in virology; Virology; Pestivirus; Classical swine fever virus; DNA suspension microarray; Virus; Microbiology; DNA chip; Method; Flaviviridae; Veterinary; Detection; Virology; Hog cholera virus

An oligonucleotide suspension microarray (Luminex ® microsphere system) was developed for detection and differentiation of animal pestiviruses: classical swine fever virus (CSFV), bovine viral diarrhea virus types 1 and 2 (BVDV1 and BVDV2), and border disease virus (BDV). Species-specific and pestiv...