A simplified method of constructing infectious clones of begomovirus employing limited restriction enzyme digestion of products of rolling circle amplification

• Published in: Journal of virological methods Vol. 147; no. 2; pp. 355 - 359
• Main Authors: Wu, Chia-Ying, Lai, Yi-Chin, Lin, Na-Sheng, Hsu, Yau-Heiu, Tsai, Hsin-Tzu, Liao, Jye-Yann, Hu, Chung-Chi
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.02.2008; Amsterdam Elsevier; New York, NY
• Subjects: Ageratum yellow vein virus; Begomovirus; Begomovirus - genetics; Biological and medical sciences; Cloning, Molecular - methods; DNA Restriction Enzymes - metabolism; Fundamental and applied biological sciences. Psychology; Genome, Viral; Infectious clone; Limited restriction enzyme digestion; Microbiology; Plant Diseases - virology; Rolling circle amplification; Squash leaf curl virus; Techniques used in virology; Tomato leaf curl virus; Virology; Infectious clone; Limited restriction enzyme digestion; Rolling circle amplification; Begomovirus; Plant pathogen; Microbiology; Enzyme; Method; Virology; Virus; Amplification; Infection; Geminiviridae

Most infectious clones of geminiviruses consist of (partial) tandem repeats of viral genomes in the vectors, which usually involve tedious, multi-step assemblies of genomic fragments in the construction process. A simplified procedure was devised to circumvent these problems, which employs limited r...