Applications of calorimetric methods to drug discovery and the study of protein interactions

• Published in: Current opinion in structural biology Vol. 13; no. 1; pp. 115 - 121
• Main Authors: Weber, Patricia C, Salemme, F Raymond
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2003
• Subjects: Calorimetry - instrumentation; Calorimetry - methods; Calorimetry, Differential Scanning - instrumentation; Calorimetry, Differential Scanning - methods; Drug Design; Energy Transfer; Entropy; Ligands; Macromolecular Substances; Protein Binding; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Proteins - chemistry; Temperature; DNSA; ITC; CBS; DSC; RalGDS

Recent studies report the application of isothermal titration calorimetry and differential scanning calorimetry to the study of protein–ligand interactions, allosteric cooperativity and aspects of protein folding. New methods of data analysis compare alternative methods for determining ligand bindin...