Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris - Shi, Xianzong; Karkut, Tammy; Chamankhah, Mahmood; Alting-Mees, Michelle; Hemmingsen, Sean M; Hegedus, Dwayne (Protein expression and purification, 2003)
Keywords: Animals; Blotting, Western; Cloning, Molecular; Colony Count, Microbial; Culture Media - pharmacology; Electrophoresis, Polyacrylamide Gel; Endopeptidases - genetics; Endopeptidases - metabolism; Hydrogen-Ion Concentration; Immunoglobulin Fragments - genetics; Immunoglobulin Fragments - metabolism; Methanol - pharmacology; Moths - chemistry; Osmotic Pressure; Pichia - cytology; Pichia - genetics; Pichia pastoris; Recombinant Proteins - drug effects; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; scFv; Serpin; Serpins - immunology; Single-chain antibody; Temperature; Time Factors; Single-chain antibody; Serpin; Pichia pastoris; scFv