Reversible Intracellular Gelation of MCF10A Cells Enables Programmable Control Over 3D Spheroid Growth

• Published in: Advanced healthcare materials Vol. 13; no. 7; p. e2302528
• Main Authors: McNally, Delaney L, Macdougall, Laura J, Kirkpatrick, Bruce E, Maduka, Chima V, Hoffman, Timothy E, Fairbanks, Benjamin D, Bowman, Christopher N, Spencer, Sabrina L, Anseth, Kristi S
• Format: Journal Article
• Language: English
• Published: Germany 01.03.2024
• Subjects: Animals; Cell Survival; Hydrogels - pharmacology; Mammals; Polyethylene Glycols - pharmacology; intracellular crosslinking; biostasis; metabolism; spheroids; hydrogel

In nature, some organisms survive extreme environments by inducing a biostatic state wherein cellular contents are effectively vitrified. Recently, a synthetic biostatic state in mammalian cells is achieved via intracellular network formation using bio-orthogonal strain-promoted azide-alkyne cycload...