Mutations of Trp275 and Trp397 altered the binding selectivity of Vibrio carchariae chitinase A - Suginta, Wipa; Songsiriritthigul, Chomphunuch; Kobdaj, Archara; Opassiri, Rodjana; Svasti, Jisnuson (Biochimica et biophysica acta, 2007)
Keywords: Active-site mutation; Binding Sites; Biological Assay; Chitin - metabolism; Chitin hydrolysis; Chitinase A; Chitinases - chemistry; Chitinases - metabolism; Chitooligosaccharide; Chromatography, Thin Layer; Circular Dichroism; Escherichia coli - genetics; Hydrolysis; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Binding; Protein Conformation; Specific hydrolyzing activity; Substrate Specificity; Thin layer chromatography; Tryptophan - chemistry; Tryptophan - genetics; Vibrio - enzymology; Vibrio carchariae; Chitinase A; Gn; TLC; DMAB; Thin layer chromatography; Vibrio carchariae; PMSF; Chitin hydrolysis; Chitooligosaccharide; IPTG; Specific hydrolyzing activity; Active-site mutation; pNP-(GlcNAc) 2