Optimized osteogenic differentiation protocol from R1 mouse embryonic stem cells in vitro

• Published in: Differentiation (London) Vol. 89; no. 1-2; pp. 1 - 10
• Main Authors: Yu, Yanhong, Al-Mansoori, Layla, Opas, Michal
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.01.2015
• Subjects: Animals; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Lineage; Dexamethasone - administration & dosage; Embryonic stem cells; In vitro culture; In Vitro Techniques - methods; Mice; Mouse Embryonic Stem Cells - drug effects; Osteoblasts - cytology; Osteoblasts - metabolism; Osteogenesis - drug effects; Osteogenesis - genetics; Osteogenic differentiation; PPAR gamma - antagonists & inhibitors; Tretinoin - administration & dosage; AA; Alp; In vitro culture; Embryonic stem cells; RA; ESC; Osx; Dex; Ppar; Ocn; EB; Osteogenic differentiation; Bsp; B-Gly; Bmp-2; Runx2

Embryonic stem cells (ESCs) are a unique model that allows the study of molecular pathways underlying commitment and differentiation. One such lineage is osteoblasts, which are responsible for forming bone tissue in the body. There are many osteogenic differentiation protocols in the literature util...