G-protein-coupled Receptor Stimulation of the p42/p44 Mitogen-activated Protein Kinase Pathway Is Attenuated by Lipid Phosphate Phosphatases 1, 1a, and 2 in Human Embryonic Kidney 293 Cells

• Published in: The Journal of biological chemistry Vol. 276; no. 16; pp. 13452 - 13460
• Main Authors: Alderton, Forbes, Darroch, Peter, Sambi, Balwinder, McKie, Amanda, Ahmed, Ikhlas Said, Pyne, Nigel, Pyne, Susan
• Format: Journal Article
• Language: English
• Published: United States American Society for Biochemistry and Molecular Biology 20.04.2001
• Subjects: Cell Line; Cell Membrane - enzymology; GTP-Binding Proteins - metabolism; Humans; Isoenzymes - metabolism; Kidney; Kinetics; Lysophospholipids - pharmacology; MAP Kinase Signaling System - physiology; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases - metabolism; Phorbol 12,13-Dibutyrate - pharmacology; Phosphatidate Phosphatase - metabolism; Phosphatidic Acids - pharmacology; Phospholipase D - metabolism; Receptors, Cell Surface - drug effects; Receptors, Cell Surface - physiology; Recombinant Proteins - metabolism; Sphingosine - analogs & derivatives; Sphingosine - pharmacology; Substrate Specificity; Tetradecanoylphorbol Acetate - pharmacology; Transfection; Virulence Factors, Bordetella - pharmacology

Sphingosine 1-phosphate, lysophosphatidic acid, and phosphatidic acid bind to G-protein-coupled receptors to stimulate intracellular signaling in mammalian cells. Lipid phosphate phosphatases (1, 1a, 2, and 3) are a group of enzymes that catalyze de-phosphorylation of these lipid agonists. It has be...