Quantitative cellular uptake, localization and cytotoxicity of curcumin in normal and tumor cells

• Published in: Biochimica et biophysica acta Vol. 1780; no. 4; pp. 673 - 679
• Main Authors: Kunwar, A., Barik, A., Mishra, B., Rathinasamy, K., Pandey, R., Priyadarsini, K.I.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2008
• Subjects: Animals; Biological Transport; Cell Line, Tumor; Cell Membrane - metabolism; Cell Nucleus - metabolism; Cell Survival - drug effects; Cells, Cultured; Cellular uptake; Curcuma - chemistry; Curcumin; Curcumin - metabolism; Curcumin - pharmacokinetics; Curcumin - pharmacology; Cytotoxicity; Dose-Response Relationship, Drug; Fluorescence; Humans; Lymphocytes - cytology; Lymphocytes - drug effects; Lymphocytes - metabolism; Mice; Microscopy, Confocal; NIH 3T3 Cells; Nuclear localization; Spectrometry, Fluorescence; Time Factors; Tumor cell; Fluorescence; Cytotoxicity; Cellular uptake; Curcumin; Nuclear localization; Tumor cell

Using absorption and fluorescence spectroscopic methods, quantitative cellular uptake of curcumin, an antioxidant and anti-tumor agent from Curcuma longa, was calculated in two types of normal cells: spleen lymphocytes, and NIH3T3 and two tumor cell lines: EL4 and MCF7. Both the uptake and fluoresce...