Oxygen-Induced Embryopathy and the Significance of Glutathione-Dependent Antioxidant System in the Rat Embryo During Early Organogenesis

• Published in: Free radical biology & medicine Vol. 22; no. 3; pp. 447 - 454
• Main Authors: Ishibashi, Miwa, Akazawa, Shoichi, Sakamaki, Hiroyuki, Matsumoto, Kazunari, Yamasaki, Hironori, Yamaguchi, Yoshihiko, Goto, Shinji, Urata, Yoshishige, Kondo, Takahito, Nagataki, Shigenobu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 1997
• Subjects: Animals; Antioxidants; Culture Techniques; Embryo culture; Embryo, Mammalian - metabolism; Embryonic and Fetal Development; Female; Fetal Diseases - chemically induced; Free radicals; Glutamate-Cysteine Ligase - genetics; Glutamate-Cysteine Ligase - metabolism; Glutathione (GSH); Glutathione - pharmacology; Glutathione peroxidase (GPX); Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Glutathione-S transferase (GST); Humans; Hydrogen peroxide (H 2O 2); Male; Neural tube defects; Neural Tube Defects - chemically induced; Oxidative stress; Oxygen - toxicity; Rats; Rats, Wistar; RNA, Messenger - metabolism; γ-Glutamylcysteine synthetase ( γ-GCS); Oxidative stress; Free radicals; Glutathione peroxidase (GPX); Embryo culture; Neural tube defects; γ-Glutamylcysteine synthetase ( γ-GCS); Glutathione-S transferase (GST); Hydrogen peroxide (H 2O 2); Glutathione (GSH)

We investigated the effect of glutathione (GSH)-dependent antioxidant system against hydrogen peroxide (H 2O 2) formation in oxygen-induced embryopathy. Exposure of rat embryos to a high concentration of oxygen (20%) during early neurulation (day 9 to 10) significantly increased the incidence of neu...