Downregulation of GPX8 in hepatocellular carcinoma: impact on tumor stemness and migration

• Published in: Cellular oncology (Dordrecht) Vol. 47; no. 4; pp. 1391 - 1403
• Main Authors: Tao, Chen-Yang, Wu, Xiao-Ling, Song, Shu-Shu, Tang, Zheng, Zhou, Yu-Fu, Tian, Meng-Xin, Jiang, Xi-Fei, Fang, Yuan, Zhu, Gui-Qi, Huang, Run, Qu, Wei-Feng, Gao, Jun, Chu, Tian-Hao, Yang, Rui, Chen, Jia-Feng, Zhao, Qian-Fu, Ding, Zhen-Bin, Dai, Zhi, Zhou, Jian, Liu, Wei-Ren, Shi, Ying-Hong, Fan, Jia
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2024; Springer Nature B.V
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Antitumor activity; Biomedical and Life Sciences; Biomedicine; Cancer Research; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - metabolism; Carcinoma, Hepatocellular - pathology; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Down-regulation; Down-Regulation - genetics; Endoplasmic reticulum; Female; Gene Expression Regulation, Neoplastic; Glutathione; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Heat shock proteins; Hepatocellular carcinoma; Hsc70 protein; Humans; Immunoprecipitation; Kinases; KLF4 protein; Kruppel-Like Factor 4; Liver cancer; Liver Neoplasms - genetics; Liver Neoplasms - metabolism; Liver Neoplasms - pathology; Male; Mass spectroscopy; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Neoplastic Stem Cells - metabolism; Neoplastic Stem Cells - pathology; Oct-4 protein; Oncology; Pathology; Phosphorylation; Pluripotency; Protein arrays; Protein transport; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Signal transduction; Signal Transduction - genetics; Therapeutic targets; Transcriptomes; Tumors; GPX8; Hepatocellular carcinoma; Hsc70; Migration; Tumor stemness

Purpose GPX8, which is found in the endoplasmic reticulum lumen, is a member of the Glutathione Peroxidases (GPXs) family. Its role in hepatocellular carcinoma (HCC) is unknown. Methods Immunohistochemical staining was used to detect the protein levels of GPX8 in HCC tissue microarrays. A short hair...