Cloning, expression and functional analysis of the duck Toll-like receptor 5 (TLR5) gene

• Published in: Journal of veterinary science (Suwŏn-si, Korea) Vol. 16; no. 1; pp. 37 - 46
• Main Authors: Cheng, Yuqiang, Sun, Yingjie, Wang, Hengan, Shi, Shuduan, Yan, Yaxian, Li, Jing, Ding, Chan, Sun, Jianhe
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한수의학회 2015; The Korean Society of Veterinary Science
• Subjects: 2',5'-Oligoadenylate Synthetase - genetics; 2',5'-Oligoadenylate Synthetase - metabolism; Animals; Cell Line; Cloning, Molecular; Ducks; Gene Expression Regulation - physiology; Humans; Immunity, Innate; Myeloid Differentiation Factor 88 - genetics; Myeloid Differentiation Factor 88 - metabolism; Myxovirus Resistance Proteins - genetics; Myxovirus Resistance Proteins - metabolism; Newcastle Disease - metabolism; Newcastle disease virus - classification; Original; RNA, Messenger - genetics; RNA, Messenger - metabolism; Species Specificity; Toll-Like Receptor 5 - genetics; Toll-Like Receptor 5 - metabolism; myeloid differentiation factor 88; Toll-like receptor 5; Newcastle disease virus; innate immune response; duck

Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In the present study, the first TLR5 gene in duck was cloned. The open reading frame (ORF) of duck TLR5 (dTLR5) cDNA is 2580 bp and encodes a polypeptide of 859 amino acids. We also cloned partial s...