Molecular recognition of DNA by small molecules: AT base pair specific intercalative binding of cytotoxic plant alkaloid palmatine

• Published in: Biochimica et biophysica acta Vol. 1770; no. 7; pp. 1071 - 1080
• Main Authors: Bhadra, Kakali, Maiti, Motilal, Kumar, Gopinatha Suresh
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2007
• Subjects: AT specific binding; Berberine Alkaloids - chemistry; Berberine Alkaloids - metabolism; Calorimetry; Circular Dichroism; Dialysis; DNA - metabolism; Fluorescence; Intercalating Agents - chemistry; Intercalating Agents - metabolism; Intercalation; Molecular Structure; Palmatine; Polynucleotides; Spectroscopy; Spectrum Analysis; Transition Temperature; Viscosity; Viscosity; CD; Spectroscopy; D/P; P/D; ESI-MS; poly(A); CCS Col E 1 DNA; Intercalation; Polynucleotides; AT; DNA; AT specific binding; Palmatine; ITC; Tm; Calorimetry; GC

The base dependent binding of the cytotoxic alkaloid palmatine to four synthetic polynucleotides, poly(dA).poly(dT), poly(dA–dT).poly(dA–dT), poly(dG).poly(dC) and poly(dG–dC).poly(dG–dC) was examined by competition dialysis, spectrophotometric, spectrofluorimetric, thermal melting, circular dichroi...