High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis

• Published in: Blood Vol. 103; no. 3; pp. 1089 - 1098
• Main Authors: Uchida, Kanji, Nakata, Koh, Trapnell, Bruce C., Terakawa, Takahiro, Hamano, Emi, Mikami, Ayako, Matsushita, Ikumi, Seymour, John F., Oh-eda, Masayoshi, Ishige, Ikuo, Eishi, Yoshinobu, Kitamura, Takayuki, Yamada, Yoshitsugu, Hanaoka, Kazuo, Keicho, Naoto
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.02.2004; The Americain Society of Hematology
• Subjects: Adult; Aged; Animals; Antibody Affinity; Antibody Specificity; Antigen-Antibody Complex - metabolism; Autoantibodies - metabolism; Biological and medical sciences; Bronchoalveolar Lavage Fluid - cytology; Bronchoalveolar Lavage Fluid - immunology; Case-Control Studies; Cell Division; Cells, Cultured; Epitopes - chemistry; Female; General aspects; Granulocyte-Macrophage Colony-Stimulating Factor - antagonists & inhibitors; Granulocyte-Macrophage Colony-Stimulating Factor - chemistry; Granulocyte-Macrophage Colony-Stimulating Factor - immunology; Humans; Immunopathology; Lung - immunology; Macrophages, Alveolar - immunology; Macrophages, Alveolar - pathology; Male; Medical sciences; Mice; Middle Aged; Models, Molecular; Neutralization Tests; Pneumology; Protein Conformation; Pulmonary Alveolar Proteinosis - etiology; Pulmonary Alveolar Proteinosis - immunology; Respiratory system : syndromes and miscellaneous diseases; Autoimmunity; Human; Lung disease; Respiratory disease; Antibody; Pathogenesis; Cytokine; Idiopathic; Autoantibody; Respiratory system; Pulmonary alveolar proteinosis; Pulmonary surfactant; Polypeptide; Granulocyte macrophage colony stimulating factor

Deficiency of granulocyte-macrophage colony-stimulating factor (GM-CSF) in mice results in pulmonary alveolar proteinosis (PAP) from impaired surfactant catabolism by alveolar macrophages (AMs). Recently, we have shown that neutralizing anti-GM-CSF autoantibodies develop specifically in patients wit...