Immunohistochemical demonstration of cholesterol side-chain cleavage cytochrome P-450 in bovine and human adrenals

• Published in: Pathology, research and practice Vol. 184; no. 3; p. 337
• Main Authors: Sasano, H, Sasano, N, Okamoto, M
• Format: Journal Article
• Language: English
• Published: Germany 01.03.1989
• Subjects: Adenoma - metabolism; Adenoma - pathology; Adrenal Cortex - metabolism; Adrenal Cortex - pathology; Adrenal Cortex Neoplasms - metabolism; Adrenal Cortex Neoplasms - pathology; Adrenal Glands - metabolism; Aldosterone - metabolism; Animals; Cattle; Cushing Syndrome - metabolism; Cytochrome P-450 Enzyme System - isolation & purification; Cytochrome P-450 Enzyme System - metabolism; Glucocorticoids - metabolism; Humans; Hyperaldosteronism - metabolism; Hyperplasia; Immunohistochemistry; Mineralocorticoids - metabolism; Zona Fasciculata - metabolism; Zona Glomerulosa - metabolism

Cytochrome P-450 specific for cholesterol side-chain cleavage (P-450SCC) was purified from the bovine adrenal and a specific antibody was raised in rabbits. The antiserum was applied for immunohistochemical visualization of the P-450SCC in the bovine and human adrenal cortex. The immunoreactivity wa...