Identification of parotid salivary biomarkers in Sjögren's syndrome by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and two-dimensional difference gel electrophoresis

• Published in: Rheumatology (Oxford, England) Vol. 45; no. 9; pp. 1077 - 1086
• Main Authors: Ryu, O. H., Atkinson, J. C., Hoehn, G. T., Illei, G. G., Hart, T. C.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.09.2006; Oxford Publishing Limited (England)
• Subjects: Amylases - analysis; beta 2-Microglobulin - analysis; Biological and medical sciences; Biomarkers - analysis; Carbonic Anhydrases - analysis; Case-Control Studies; Cystatin C; Cystatins - analysis; Diseases of the osteoarticular system; Electrophoresis, Gel, Two-Dimensional - methods; Humans; Lactoferrin - analysis; Medical sciences; Muramidase - analysis; Parotid Gland; Protein Subunits - analysis; Proteomics; Receptors, Polymeric Immunoglobulin - analysis; Saliva - chemistry; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Sjogren's Syndrome - diagnosis; Sjogren's Syndrome - metabolism; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Statistics, Nonparametric; SELDI-TOF-MS; Immunopathology; Parotid gland; Stomatology; Gel electrophoresis; 2D-DIGE; Rheumatology; Exploration; Autoimmune disease; Identification; Sjögren syndrome; Laser desorption; Eye disease; Sjögren's syndrome; Systemic disease; Proteomics; Saliva; Mass spectrometry

To identify the most significant salivary biomarkers in Sjögren's syndrome (SS) using proteomic methods. Parotid saliva from 20 non-SS subjects and 41 primary SS patients was analysed. Protein expression profiles for each sample were generated by surface-enhanced laser desorption/ionization tim...