Focal adhesion kinase as well as p130Cas and paxillin is crucially involved in the enhanced malignant properties under expression of ganglioside GD3 in melanoma cells

• Published in: Biochimica et biophysica acta Vol. 1780; no. 3; pp. 513 - 519
• Main Authors: Hamamura, Kazunori, Tsuji, Momoko, Ohkawa, Yuki, Nakashima, Hideyuki, Miyazaki, Sayaka, Urano, Takeshi, Yamamoto, Noriyuki, Ueda, Minoru, Furukawa, Koichi, Furukawa, Keiko
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2008
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - pharmacology; Cattle; Cell Line, Tumor; Cell Proliferation - drug effects; Crk-Associated Substrate Protein - metabolism; Focal adhesion kinase; Focal Adhesion Protein-Tyrosine Kinases - chemistry; Focal Adhesion Protein-Tyrosine Kinases - metabolism; Ganglioside; Gangliosides - metabolism; GD3; Humans; Immunoprecipitation; Invasion; Mass Spectrometry; Melanoma; Melanoma - enzymology; Melanoma - pathology; Molecular Sequence Data; Mutation - genetics; Neoplasm Invasiveness; Paxillin - metabolism; Phosphorylation - drug effects; Phosphotyrosine - metabolism; Proliferation; Protein Binding - drug effects; Serum; Transfection; Tyrosine phosphorylation; Ganglioside; Tyrosine phosphorylation; GD3; Melanoma; Focal adhesion kinase; Proliferation; Invasion

Mass spectrometry analysis of immunoprecipitates from serum-treated GD3-expressing melanoma cells with PY20 (anti-phosphotyrosine antibody) revealed that focal adhesion kinase (FAK) is more strongly activated in GD3-expressing cells than in GD3-negative cells. Involvent of FAK in the increased proli...