Proteomic analysis of crocodile white blood cells reveals insights into the mechanism of the innate immune system

• Published in: Heliyon Vol. 10; no. 2; p. e24583
• Main Authors: Tankrathok, Anupong, Mahong, Bancha, Roytrakul, Sittiruk, Daduang, Sakda, Temsiripong, Yosapong, Klaynongsruang, Sompong, Jangpromma, Nisachon
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 30.01.2024; Elsevier
• Subjects: Aeromonas hydrophila; Crocodylus siamensis; Gel-free proteomics; Innate immunity; Aeromonas hydrophila; Innate immunity; Crocodylus siamensis; Gel-free proteomics
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e24583

Crocodiles have a particularly powerful innate immune system because their blood contains high levels of antimicrobial peptides. They can survive injuries that would be fatal to other animals, and they are rarely afflicted with diseases. To better understand the crocodile’s innate immune response, proteomic analysis was performed on the white blood cells (WBC) of an Aeromonas hydrophila-infected crocodile. Levels of WBC and red blood cells (RBC) rapidly increased within 1 h. In WBC, there were 109 up-regulated differentially expressed proteins (DEP) that were up-regulated. Fifty-nine DEPs dramatically increased expression from 1 h after inoculation, whereas 50 up-regulated DEPs rose after 24 h. The most abundant DEPs mainly had two biological functions, 1) gene expression regulators, for example, zinc finger proteins and histone H1 family, and 2) cell mechanical forces such as actin cytoskeleton proteins and microtubule-binding proteins. This finding illustrates the characteristic effective innate immune response mechanism of crocodiles that might occur via boosted transcription machinery proteins to accelerate cytoskeletal protein production for induction of phagocytosis, along with the increment of trafficking proteins to transport essential molecules for combating pathogens. The findings of this study provide new insights into the mechanisms of the crocodile’s innate immune system.