Extracellular matrix stiffness regulates degradation of MST2 via SCF βTrCP

• Published in: Biochimica et biophysica acta. General subjects Vol. 1866; no. 12; p. 130238
• Main Authors: Fiore, Ana Paula Zen Petisco, Rodrigues, Ana Maria, Ribeiro-Filho, Helder Veras, Manucci, Antonio Carlos, de Freitas Ribeiro, Pedro, Botelho, Mayara Carolinne Silva, Vogel, Christine, Lopes-de-Oliveira, Paulo Sergio, Pagano, Michele, Bruni-Cardoso, Alexandre
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2022
• Subjects: beta-Transducin Repeat-Containing Proteins - metabolism; Breast cells; breasts; carcinogenesis; epithelium; extracellular matrix; Extracellular Matrix - metabolism; Extracellular matrix stiffness; Hippo; Humans; integrins; molecular dynamics; MST2; non-specific serine/threonine protein kinase; Phosphorylation; Proteolysis; SCF βTrCP; Serine-Threonine Kinase 3 - metabolism; site-directed mutagenesis; ubiquitin-protein ligase; Ubiquitin-Protein Ligases - metabolism; Ubiquitinproteasome system; MST2; Ubiquitinproteasome system; SCF βTrCP; Extracellular matrix stiffness; Breast cells; Hippo
• ISSN: 0304-4165; 1872-8006; 1872-8006
• DOI: 10.1016/j.bbagen.2022.130238

The Hippo pathway plays central roles in relaying mechanical signals during development and tumorigenesis, but how the proteostasis of the Hippo kinase MST2 is regulated remains unknown. Here, we found that chemical inhibition of proteasomal proteolysis resulted in increased levels of MST2 in human...