Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system

• Published in: Journal of biotechnology Vol. 146; no. 4; pp. 160 - 168
• Main Authors: Lwa, Teng Rhui, Tan, Chuan Hao, Lew, Qiao Jing, Chu, Kai Ling, Tan, Janice, Lee, Yih Yean, Chao, Sheng-Hao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.04.2010; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - metabolism; Biological and medical sciences; Biotechnology; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; CEBPG; Cell Growth Processes - physiology; Cell Line; CHO; CHO Cells; Cricetinae; Cricetulus; EPO; Erythropoietin - genetics; Erythropoietin - metabolism; Fundamental and applied biological sciences. Psychology; Gaussia; HEK293; Humans; Interferon-gamma - genetics; Interferon-gamma - metabolism; Luciferases - genetics; Luciferases - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Monoclonal antibody; Potassium Channels, Voltage-Gated - genetics; Potassium Channels, Voltage-Gated - metabolism; Protein Engineering - methods; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; RNA, Small Interfering - genetics; siRNA; HEK293; siRNA; CHO; Monoclonal antibody; EPO; CEBPG; RNA interference; Enzyme; Luciferase; Identification; CHO cell line; Gene silencing; Screening; Gene; Production; Oxidoreductases; Recombinant protein

Development of high-throughput functional genomic screening, including siRNA screening, provides a novel approach for quick identification of critical factors involved in biological processes. Here, we apply this strategy to search for cellular genes involved in recombinant protein production. Since...