Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system

• Published in: Journal of biotechnology Vol. 146; no. 4; pp. 160 - 168
• Main Authors: Lwa, Teng Rhui, Tan, Chuan Hao, Lew, Qiao Jing, Chu, Kai Ling, Tan, Janice, Lee, Yih Yean, Chao, Sheng-Hao
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.04.2010; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - metabolism; Biological and medical sciences; Biotechnology; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; CEBPG; Cell Growth Processes - physiology; Cell Line; CHO; CHO Cells; Cricetinae; Cricetulus; EPO; Erythropoietin - genetics; Erythropoietin - metabolism; Fundamental and applied biological sciences. Psychology; Gaussia; HEK293; Humans; Interferon-gamma - genetics; Interferon-gamma - metabolism; Luciferases - genetics; Luciferases - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Monoclonal antibody; Potassium Channels, Voltage-Gated - genetics; Potassium Channels, Voltage-Gated - metabolism; Protein Engineering - methods; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; RNA, Small Interfering - genetics; siRNA; HEK293; siRNA; CHO; Monoclonal antibody; EPO; CEBPG; RNA interference; Enzyme; Luciferase; Identification; CHO cell line; Gene silencing; Screening; Gene; Production; Oxidoreductases; Recombinant protein
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2010.02.016

Development of high-throughput functional genomic screening, including siRNA screening, provides a novel approach for quick identification of critical factors involved in biological processes. Here, we apply this strategy to search for cellular genes involved in recombinant protein production. Since most of biopharmaceutical proteins are secreted proteins, we develop a cell-based reporter assay using a secreted luciferase, Gaussia luciferase (Gluc), as the reporter. Human embryonic kidney 293 (HEK293) cells transiently transfected with the Gluc reporter plasmid are used to screen our siRNA panel. Three cellular genes, CCAAT/enhancer binding protein gamma (CEBPG), potassium channel tetramerisation domain containing 2 (KCTD2), transmembrane protein 183A (TMEM183A), were isolated from the screening. Production of erythropoietin (EPO) was significantly inhibited when CEBPG, KCTD2, and TMEM183A were knocked down. Furthermore, overexpression of CEBPG is shown to significantly improve production of recombinant EPO, interferon γ, and monoclonal antibody in HEK293 and Chinese hamster ovary cells. Collectively, this novel Gluc-based siRNA screening system is proven to be a useful tool for investigation of secreted protein production in mammalian cells.