High Homocysteine-Thiolactone Leads to Reduced MENIN Protein Expression and an Impaired DNA Damage Response: Implications for Neural Tube Defects

• Published in: Molecular neurobiology Vol. 61; no. 10; pp. 7369 - 7383
• Main Authors: Bai, Baoling, Wan, Chunlei, Xiao, Zonghui, Li, Dan, Liu, Lingyun, Zhang, Kexin, Zhang, Ting, Zhang, Qin
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2024; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Birth defects; Cell Biology; Chick Embryo; CHK1 protein; Chromatin; DNA Damage; DNA Repair - drug effects; DNA-directed RNA polymerase; Epigenesis, Genetic - drug effects; Epigenetics; Fetuses; Folic acid; Histones; Histones - metabolism; Homocysteine; Homocysteine - analogs & derivatives; Hyperhomocysteinemia; Immunofluorescence; Immunoprecipitation; Investigations; Mice; Neural tube defects; Neural Tube Defects - genetics; Neural Tube Defects - metabolism; Neurobiology; Neuroectoderm; Neurology; Neurosciences; Nucleotide excision repair; Original; Original Article; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; Risk factors; RNA-mediated interference; Thiolactone; Western blotting; Histone 3 lysine 79 homocysteinylation (H3K79hcy); Hyperhomocysteinemia (HHcy); Neural tube defects; Homocysteine-thiolactone (HTL); Menin; DNA damage response (DDR); Histone 3 lysine 4 trimethylation (H3K4me3); Men1/Menin

DNA damage is associated with hyperhomocysteinemia (HHcy) and neural tube defects (NTDs). Additionally, HHcy is a risk factor for NTDs. Therefore, this study examined whether DNA damage is involved in HHcy-induced NTDs and investigated the underlying pathological mechanisms involved. Embryonic day 9...