920 MHz ultra-high field NMR approaches to structural glycobiology

• Published in: Biochimica et biophysica acta Vol. 1780; no. 3; pp. 619 - 625
• Main Authors: Kato, Koichi, Sasakawa, Hiroaki, Kamiya, Yukiko, Utsumi, Maho, Nakano, Michiko, Takahashi, Noriko, Yamaguchi, Yoshiki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2008
• Subjects: Amyloid beta-Peptides - chemistry; Amyloid β; Animals; Ganglioside GM1; Glycolipid; Glycomics - methods; Glycoprotein; Glycoproteins - chemistry; Humans; Isotope Labeling; Magnetic Resonance Spectroscopy - methods; Mice; Oligosaccharide; Oligosaccharides - chemistry; Stable-isotope labeling; Structural glycobiology; Ultra-high field NMR spectroscopy; CD; NOE; Oligosaccharide; Glycoprotein; Ganglioside GM1; Structural glycobiology; Stable-isotope labeling; Glycolipid; CSA; RDC; Aβ; 3D; PA; TROSY; NMR; Ultra-high field NMR spectroscopy; Amyloid β; protein; HSQC; Ig

Although NMR spectroscopy has great potential to provide us with detailed structural information on oligosaccharides and glycoconjugates, the carbohydrate NMR analyses have been hampered by the severe spectral overlapping and the insufficiency of the conformational restraints. Recently, ultra-high f...