Automation in high-content flow cytometry screening

• Published in: Cytometry. Part A Vol. 75A; no. 9; pp. 789 - 797
• Main Authors: Naumann, U, Wand, M.P
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.09.2009
• Subjects: Acute Disease; Automation - instrumentation; Automation - methods; Blood Component Transfusion; Bone Marrow Transplantation; Computational Biology; density curvature; Flow Cytometry - instrumentation; Flow Cytometry - methods; Graft vs Host Disease - blood; Graft vs Host Disease - pathology; high throughput flow cytometry; highest density region; Humans; penalised splines; Reproducibility of Results; Robotics - instrumentation; Robotics - methods; subject specific curves

High-content flow cytometric screening (FC-HCS) is a 21st Century technology that combines robotic fluid handling, flow cytometric instrumentation, and bioinformatics software, so that relatively large numbers of flow cytometric samples can be processed and analysed in a short period of time. We rev...