Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry

• Published in: Cytometry. Part A Vol. 75A; no. 5; pp. 440 - 449
• Main Authors: Gilbert, Daniel F, Wilson, John C, Nink, Virginia, Lynch, Joseph W, Osborne, Geoffrey W
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2009
• Subjects: Cell Line; Cell Survival; chloride channels; Flow Cytometry - methods; glycine receptor; Humans; multiplex; Mutation; Receptors, Glycine - biosynthesis; Receptors, Glycine - genetics; Sensitivity and Specificity; Transfection

Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this method is not suited to assays requiring functio...