Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system - 龚兴国 纪静 谢捷 周远 张俊彦 钟文涛 (Journal of Zhejiang University. B. Science, 2006)
Keywords: Bacteria; Bacterial Proteins - biosynthesis; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Biotechnology & Food Sciences; Glutathione Transferase - biosynthesis; Glutathione Transferase - genetics; Glutathione Transferase - isolation & purification; Kinases; Oncogene Protein pp60(v-src) - biosynthesis; Oncogene Protein pp60(v-src) - chemistry; Oncogene Protein pp60(v-src) - genetics; Oncogene Protein pp60(v-src) - isolation & purification; Protein Engineering - methods; Proteins; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - isolation & purification; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Signal transduction; Protein tyrosine kinase; v-Src; Inclusion body; Orthogonalization; GST-fusion