Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system
v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST （glutathione S-transferase）-fused v-Src from a bacterial expression s...
Saved in:
| Published in | Journal of Zhejiang University. B. Science Vol. 7; no. 1; pp. 13 - 19 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
China
Springer Nature B.V
2006
Institute of Biomacromolecule and Enzyme Engineering, School of Life Sciences, Zhejiang University, Hangzhou 310027, China Zhejiang University Press |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST （glutathione S-transferase）-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl ,B-D-thiogalactopyranoside （IPTG）-regulated expression, and the fused protein was purified using GSH （glutathione） affinity chromatography. ELISA （enzyme-linked immunosorbent assay） was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. |
|---|---|
| AbstractList | v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST （glutathione S-transferase）-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl ,B-D-thiogalactopyranoside （IPTG）-regulated expression, and the fused protein was purified using GSH （glutathione） affinity chromatography. ELISA （enzyme-linked immunosorbent assay） was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl beta-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.[PUBLICATION ABSTRACT] Q556+.5%Q786; v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present herethe expression, purification, and bioactivity of a GST (glutathione S-transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β-D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST (glutathione S -transferase)-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl β -D-thiogalactopyranoside (IPTG)-regulated expression, and the fused protein was purified using GSH (glutathione) affinity chromatography. ELISA (enzyme-linked immunosorbent assay) was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression. |
| Author | 龚兴国 纪静 谢捷 周远 张俊彦 钟文涛 |
| AuthorAffiliation | Institute of Biomacromolecule and Enzyme Engineering, School of Life Sciences, Zhejiang University, Hangzhou 310027, China |
| AuthorAffiliation_xml | – name: Institute of Biomacromolecule and Enzyme Engineering, School of Life Sciences, Zhejiang University, Hangzhou 310027, China |
| Author_xml | – sequence: 1 fullname: 龚兴国 纪静 谢捷 周远 张俊彦 钟文涛 |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/16365920$$D View this record in MEDLINE/PubMed |
| BookMark | eNqFks1v0zAYxi00xD7gzA1FHDgg0vkjjpPLJJjGQJrEYeNsOc7rziWxOzsp7f56nLZUMAlxsi3_nuf9PEVHzjtA6DXBM1Iycr54HOOMYlzOPmFM2DN0QqqS5kRU7CjdS8FywityjE5jXGBcFFiUL9Bx0pa8pvgEyav1MkCM1rsP2XIM1lithu1LuTZrrFd6sCs7bDJvsuvbu9yMEdpsld8GnZng-0xlTWIgWNVlcHDL4iYO0L9Ez43qIrzan2fo--eru8sv-c2366-XH29yXdCS5ZRXtdDAU4ptQVrDOS-M4dCKlgIFbnSjqGZtUSuBVVMRYphpqMCCGtCasDN0sfNdjk0PrQY3BNXJZbC9ChvplZV__zh7L-d-JQkrieBFMni_M_ipnFFuLhd-DC6lLB8X7XrdNBKmPmOCMUvwu3204B9GiIPsbdTQdcqBH6MsRWo6x_y_IMWCYVJXCXz7BDzEryrGasrE5PbmzyIP1f0eZwL4DtDBxxjASG2H7ThTzbaTBE8skdPayKkcuV2bpDt_ojtY_1Oxz1ffezd_sKlhaQt-GNuBpKSoMS0E-wVnq9HK |
| CitedBy_id | crossref_primary_10_1016_j_bbrc_2024_150774 crossref_primary_10_1002_bab_1749 crossref_primary_10_1007_s11274_013_1253_0 |
| Cites_doi | 10.1016/0003-2697(76)90527-3 10.1016/S0014-5793(00)02354-1 10.1002/bies.950170408 10.1016/S0165-6147(00)01567-4 10.1007/s00018-002-8438-2 10.1016/S0959-8049(00)00153-2 10.1016/S0092-8674(00)80664-9 10.1189/jlb.68.5.603 10.1038/35073094 10.4049/jimmunol.158.4.1650 10.1016/S0021-9258(19)67724-0 10.1038/ncb829 10.1186/bcr55 10.1042/bj2870985 10.1038/294771a0 10.1038/sj.onc.1204186 10.1006/abbi.1997.0236 10.1006/cbir.1994.1083 10.1016/0014-5793(93)80174-S 10.1006/prep.2000.1221 10.1016/0378-1119(88)90005-4 |
| ClassificationCodes | Q556+.5%Q786 |
| ContentType | Journal Article |
| Copyright | Springer-Verlag 2006 Copyright © Wanfang Data Co. Ltd. All Rights Reserved. Copyright © 2006, Journal of Zhejiang University Science 2006 |
| Copyright_xml | – notice: Springer-Verlag 2006 – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. – notice: Copyright © 2006, Journal of Zhejiang University Science 2006 |
| DBID | 2RA 92L CQIGP W94 WU4 ~WA AAYXX CITATION CGR CUY CVF ECM EIF NPM 3V. 7QO 7QP 7TK 7X2 7X7 7XB 88E 8AO 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI BKSAR CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ HCIFZ K9. KB. L6V LK8 M0K M0S M1P M7P M7S P5Z P62 P64 PATMY PCBAR PDBOC PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY 7QL 7T7 C1K 7X8 2B. 4A8 92I 93N PSX TCJ 5PM |
| DOI | 10.1631/jzus.2006.B0013 |
| DatabaseName | 维普期刊资源整合服务平台 中文科技期刊数据库-CALIS站点 中文科技期刊数据库-7.0平台 中文科技期刊数据库-自然科学 中文科技期刊数据库-自然科学-生物科学 中文科技期刊数据库- 镜像站点 CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed ProQuest Central (Corporate) Biotechnology Research Abstracts Calcium & Calcified Tissue Abstracts Neurosciences Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Journals Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Earth, Atmospheric & Aquatic Science Collection ProQuest One ProQuest Materials Science Collection ProQuest Central Engineering Research Database Proquest Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database ProQuest Engineering Collection Biological Sciences Agricultural Science Database ProQuest Health & Medical Collection Medical Database Biological Science Database Engineering Database ProQuest advanced technologies & aerospace journals ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Earth, Atmospheric & Aquatic Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Bacteriology Abstracts (Microbiology B) Industrial and Applied Microbiology Abstracts (Microbiology A) Environmental Sciences and Pollution Management MEDLINE - Academic Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials SciTech Premium Collection ProQuest Central China ProQuest One Applied & Life Sciences Health Research Premium Collection Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central Earth, Atmospheric & Aquatic Science Collection ProQuest Health & Medical Research Collection ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Agricultural & Environmental Science Collection Materials Science Database ProQuest Materials Science Collection ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Materials Science & Engineering Collection ProQuest Central (Alumni) Bacteriology Abstracts (Microbiology B) Industrial and Applied Microbiology Abstracts (Microbiology A) Environmental Sciences and Pollution Management MEDLINE - Academic |
| DatabaseTitleList | MEDLINE MEDLINE - Academic Agricultural Science Database Biotechnology Research Abstracts |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| DocumentTitleAlternate | Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system |
| EISSN | 1862-1783 |
| EndPage | 19 |
| ExternalDocumentID | PMC1361754 zjdxxbb_e200601003 2425188331 16365920 10_1631_jzus_2006_B0013 21490247 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GrantInformation_xml | – fundername: the National Innovation Fund for Technology, Ministry of Science and Technology of China funderid: (03C26213300586) |
| GroupedDBID | -56 -5G -BR -EM -Y2 -~C .86 .VR 06C 06D 0R~ 0VY 188 1N0 29L 29~ 2B. 2C. 2J2 2JN 2JY 2KG 2KM 2LR 2RA 2WC 30V 3V. 4.4 406 408 40D 40E 53G 5GY 5VR 5VS 67N 6NX 7X2 7X7 7XC 88E 8AO 8CJ 8FE 8FG 8FH 8FI 8FJ 8UJ 92E 92I 92L 92Q 93N 95- 95. 95~ 96X AAAVM AABHQ AAFGU AAHNG AAIAL AAJKR AAKDD AANXM AANZL AARHV AARTL AAYIU AAYQN ABBBX ABDZT ABECU ABFGW ABFTV ABHLI ABJCF ABJOX ABKAS ABKCH ABKTR ABMNI ABMQK ABNWP ABQBU ABSXP ABTEG ABTHY ABTMW ABUWG ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACHSB ACHXU ACIGE ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACPRK ACSNA ACTTH ACVWB ACWMK ADHHG ADHIR ADINQ ADKPE ADMDM ADRFC ADURQ ADYFF ADZKW AEBTG AEFTE AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESTI AETLH AEVTX AEXYK AFKRA AFLOW AFRAH AFUIB AFWTZ AFZKB AGAYW AGDGC AGGBP AGJBK AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AINHJ AITGF AJBLW AJDOV AJRNO AJZVZ AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMYQR ARAPS ARMRJ ASPBG ATCPS AVWKF AXYYD AZFZN B-. BA0 BBAFP BBNVY BDATZ BENPR BGLVJ BGNMA BHPHI BKSAR BPHCQ BVXVI CAG CCEZO CDYEO CEKLB CHBEP COF CQIGP CS3 CSCUP CW9 D1I D1J D1K DIK DNIVK DU5 E3Z EBLON EBS EIOEI EJD ESBYG F5P FA0 FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 H13 HCIFZ HF~ HG6 HH5 HLICF HMJXF HRMNR HVGLF HYE HZ~ IHE IPNFZ IXD I~X I~Z J-C JBSCW JZLTJ K6- KB. KDC KOV L6V LK5 LK8 LLZTM M0K M1P M4Y M7P M7R M7S MA- N2Q NB0 NQJWS NU0 O9- O9J OK1 OVD P62 PATMY PCBAR PDBOC PF0 PQEST PQQKQ PQUKI PROAC PSQYO PT4 PTHSS PYCSY Q2X QOR QOS R89 R9I ROL RPM RPX RSV S16 S1Z S27 S3A S3B SAP SBL SCL SDH SHX SISQX SJN SJYHP SNE SNX SOJ SPISZ SRMVM SSLCW STPWE SZN T13 TCJ TEORI TGP TR2 TSG TUC U2A U9L UG4 UGNYK UKHRP UNUBA UOJIU UTJUX UZ3 UZ4 UZ5 UZXMN VC2 VFIZW W23 W48 W94 WK8 WOQ WU4 YLTOR ZMTXR ZOVNA ~A9 ~WA -SA -S~ AACDK AAHBH AAJBT AAPKM AASML AATNV AAYXX AAYZH ABAKF ABBRH ABDBE ABFSG ABJNI ABQSL ABTKH ACAOD ACDTI ACMFV ACPIV ACSTC ACZOJ ADHKG ADTPH AEFQL AEMSY AESKC AEVLU AEZWR AFDZB AFHIU AGQEE AGQPQ AGRTI AHPBZ AHWEU AIGIU AIXLP ALIPV AMXSW AOCGG ATHPR AYFIA BSONS CAJEA CCPQU CITATION DDRTE DPUIP HMCUK IKXTQ IWAJR NPVJJ PHGZM PHGZT Q-- SNPRN SOHCF U1G U5K AAXDM CGR CUY CVF ECM EIF NPM 7QO 7QP 7TK 7XB 8FD 8FK ABRTQ AZQEC DWQXO FR3 GNUQQ K9. P64 PJZUB PKEHL PPXIY PQGLB PRINS 7QL 7T7 C1K 7X8 4A8 PMFND PSX 5PM |
| ID | FETCH-LOGICAL-c4263-25897ce5004d41df5554ff5ed7d2e2e5fcba2c3d49a70ab811f3fb27072fecc13 |
| IEDL.DBID | 7X7 |
| ISSN | 1673-1581 |
| IngestDate | Thu Aug 21 18:29:04 EDT 2025 Thu May 29 04:06:15 EDT 2025 Mon Jul 21 10:57:12 EDT 2025 Fri Jul 11 08:09:10 EDT 2025 Fri Jul 25 11:08:35 EDT 2025 Wed Feb 19 01:44:55 EST 2025 Tue Jul 01 04:06:19 EDT 2025 Thu Apr 24 23:10:43 EDT 2025 Thu Nov 24 20:32:07 EST 2022 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 1 |
| Keywords | Protein tyrosine kinase v-Src Inclusion body Orthogonalization GST-fusion |
| Language | English |
| License | http://www.springer.com/tdm |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c4263-25897ce5004d41df5554ff5ed7d2e2e5fcba2c3d49a70ab811f3fb27072fecc13 |
| Notes | Q 33-1356/Q v-Src, GST-fusion, Inclusion body, Orthogonalization, Protein tyrosine kinase ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 14 ObjectType-Article-1 ObjectType-Feature-2 content type line 23 |
| OpenAccessLink | https://link.springer.com/content/pdf/10.1631/jzus.2006.B0013.pdf |
| PMID | 16365920 |
| PQID | 883392375 |
| PQPubID | 326286 |
| PageCount | 7 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_1361754 wanfang_journals_zjdxxbb_e200601003 proquest_miscellaneous_67581505 proquest_miscellaneous_20730198 proquest_journals_883392375 pubmed_primary_16365920 crossref_citationtrail_10_1631_jzus_2006_B0013 crossref_primary_10_1631_jzus_2006_B0013 chongqing_backfile_21490247 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2006-00-00 |
| PublicationDateYYYYMMDD | 2006-01-01 |
| PublicationDate_xml | – year: 2006 text: 2006-00-00 |
| PublicationDecade | 2000 |
| PublicationPlace | China |
| PublicationPlace_xml | – name: China – name: Hangzhou |
| PublicationTitle | Journal of Zhejiang University. B. Science |
| PublicationTitleAlternate | Journal of Zhejiang University Science |
| PublicationTitle_FL | JOURNAL OF ZHEJIANG UNIVERSITY SCIENCE B |
| PublicationYear | 2006 |
| Publisher | Springer Nature B.V Institute of Biomacromolecule and Enzyme Engineering, School of Life Sciences, Zhejiang University, Hangzhou 310027, China Zhejiang University Press |
| Publisher_xml | – name: Springer Nature B.V – name: Institute of Biomacromolecule and Enzyme Engineering, School of Life Sciences, Zhejiang University, Hangzhou 310027, China – name: Zhejiang University Press |
| References | R.J. Jones (13_CR13) 2000; 36 K. Azuma (13_CR2) 1991; 266 M.Z. Korade (13_CR14) 2000; 68 M. Susva (13_CR29) 2000; 21 S. Hideyuki (13_CR11) 1993; 327 R.B. Irby (13_CR12) 2002; 62 A. Ptasznik (13_CR21) 2000; 48 E. Aviziente (13_CR1) 2002; 4 N.B. Lydon (13_CR15) 1992; 287 C.F. Margaret (13_CR17) 2002; 1602 M. Susa (13_CR28) 2000; 19 G. Superti-Furga (13_CR27) 1995; 17 M.C. Frame (13_CR9) 2002; 1602 Y.C. Ma (13_CR16) 2002; 59 J. Sambrook (13_CR23) 2002 C.F. Margaret (13_CR18) 2002; 3 J. Schlessinger (13_CR24) 2000; 100 V. Fincham (13_CR8) 1994; 18 M. Okada (13_CR20) 2000; 45 M.M. Bradford (13_CR4) 1976; 7 S. Dehm (13_CR7) 2001; 487 G.S. Martin (13_CR19) 2001; 2 T.M. Gilmer (13_CR10) 1981; 294 P.T. Ram (13_CR22) 2001; 20 J.S. Biscardi (13_CR3) 2002; 2 D.B. Smith (13_CR25) 1988; 67 G. Sun (13_CR26) 1997; 345 M.T. Brown (13_CR5) 1996; 1287 R.J. Budde (13_CR6) 1993; 23 K.E. Zoller (13_CR30) 1997; 158 11994743 - Nat Rev Mol Cell Biol. 2002 Apr;3(4):233-45 10722228 - Arch Immunol Ther Exp (Warsz). 2000;48(1):27-30 11964124 - Cell Mol Life Sci. 2002 Mar;59(3):456-62 7537961 - Bioessays. 1995 Apr;17(4):321-30 11073097 - J Leukoc Biol. 2000 Nov;68(5):603-13 6172716 - Nature. 1981 Dec 24;294(5843):771-3 12134161 - Nat Cell Biol. 2002 Aug;4(8):632-8 2002030 - J Biol Chem. 1991 Mar 15;266(8):4831-9 9281320 - Arch Biochem Biophys. 1997 Sep 1;345(1):135-42 942051 - Anal Biochem. 1976 May 7;72:248-54 3047011 - Gene. 1988 Jul 15;67(1):31-40 11313907 - Oncogene. 2001 Mar 26;20(13):1601-6 11250711 - Breast Cancer Res. 2000;2(3):203-10 8672527 - Biochim Biophys Acta. 1996 Jun 7;1287(2-3):121-49 11121839 - Trends Pharmacol Sci. 2000 Dec;21(12):489-95 9029101 - J Immunol. 1997 Feb 15;158(4):1650-9 10959046 - Eur J Cancer. 2000 Aug;36(13 Spec No):1595-606 1280108 - Biochem J. 1992 Nov 1;287 ( Pt 3):985-93 10676810 - Cell. 2000 Feb 4;100(3):293-6 10833396 - Protein Expr Purif. 2000 Jun;19(1):99-106 11389470 - Nat Rev Mol Cell Biol. 2001 Jun;2(6):467-75 7687570 - FEBS Lett. 1993 Jul 26;327(2):224-30 8049678 - Cell Biol Int. 1994 May;18(5):337-44 12020799 - Biochim Biophys Acta. 2002 Jun 21;1602(2):114-30 7504254 - Prep Biochem. 1993 Nov;23(4):493-515 11163360 - FEBS Lett. 2001 Jan 5;487(3):367-71 11185916 - Tanpakushitsu Kakusan Koso. 2000 Dec;45(16):2624-31 11980666 - Cancer Res. 2002 May 1;62(9):2669-74 |
| References_xml | – volume: 1602 start-page: 114 issue: 2 year: 2002 ident: 13_CR9 publication-title: Biochem. Biophys. Acta – volume: 7 start-page: 248 issue: 72 year: 1976 ident: 13_CR4 publication-title: Anal. Biochem. doi: 10.1016/0003-2697(76)90527-3 – volume: 487 start-page: 367 issue: 3 year: 2001 ident: 13_CR7 publication-title: FEBS Lett. doi: 10.1016/S0014-5793(00)02354-1 – volume: 17 start-page: 321 issue: 4 year: 1995 ident: 13_CR27 publication-title: Bioassays doi: 10.1002/bies.950170408 – volume: 21 start-page: 489 issue: 12 year: 2000 ident: 13_CR29 publication-title: Trends in Pharmacological Sciences doi: 10.1016/S0165-6147(00)01567-4 – volume: 23 start-page: 493 issue: 4 year: 1993 ident: 13_CR6 publication-title: Prep. Biochem. – volume: 59 start-page: 456 issue: 3 year: 2002 ident: 13_CR16 publication-title: Cell Mol. Life Sci. doi: 10.1007/s00018-002-8438-2 – volume: 36 start-page: 1595 issue: pt13 year: 2000 ident: 13_CR13 publication-title: European Journal of Cancer doi: 10.1016/S0959-8049(00)00153-2 – volume: 100 start-page: 293 issue: 3 year: 2000 ident: 13_CR24 publication-title: Cell doi: 10.1016/S0092-8674(00)80664-9 – volume: 45 start-page: 2624 issue: 16 year: 2000 ident: 13_CR20 publication-title: Tanpalushitsu Kakusan Koso – volume: 68 start-page: 603 issue: 5 year: 2000 ident: 13_CR14 publication-title: J. Leukoc. Biol. doi: 10.1189/jlb.68.5.603 – volume: 1602 start-page: 114 issue: 1 year: 2002 ident: 13_CR17 publication-title: Biochimica. et Biophysica. Acta – volume: 2 start-page: 467 issue: 6 year: 2001 ident: 13_CR19 publication-title: Nature Reviews Molecular Cell Biology doi: 10.1038/35073094 – volume: 3 start-page: 233 issue: 1 year: 2002 ident: 13_CR18 publication-title: Nature Reviews Molecular Cell Biology – volume: 48 start-page: 27 issue: 1 year: 2000 ident: 13_CR21 publication-title: Arch. Immunol. Ther. Exp. – volume: 158 start-page: 1650 issue: 4 year: 1997 ident: 13_CR30 publication-title: J. Immunol. doi: 10.4049/jimmunol.158.4.1650 – volume: 266 start-page: 4831 issue: 8 year: 1991 ident: 13_CR2 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)67724-0 – volume: 4 start-page: 632 issue: 8 year: 2002 ident: 13_CR1 publication-title: Nat. Cell Biol. doi: 10.1038/ncb829 – volume: 2 start-page: 203 issue: 3 year: 2002 ident: 13_CR3 publication-title: Breast Cancer Res. doi: 10.1186/bcr55 – volume: 1287 start-page: 121 issue: 2-3 year: 1996 ident: 13_CR5 publication-title: Biochim. Biophys. Acta – volume: 62 start-page: 2669 issue: 9 year: 2002 ident: 13_CR12 publication-title: Cancer Res. – volume: 287 start-page: 985 issue: pt3 year: 1992 ident: 13_CR15 publication-title: Biochem. J. doi: 10.1042/bj2870985 – volume: 294 start-page: 771 issue: 24 year: 1981 ident: 13_CR10 publication-title: Nature doi: 10.1038/294771a0 – volume: 20 start-page: 1601 issue: 13 year: 2001 ident: 13_CR22 publication-title: Oncogene doi: 10.1038/sj.onc.1204186 – start-page: 1723 volume-title: Molecular Cloning: A Laboratory Manual year: 2002 ident: 13_CR23 – volume: 345 start-page: 135 issue: 1 year: 1997 ident: 13_CR26 publication-title: Arch. Biochem. Biophys. doi: 10.1006/abbi.1997.0236 – volume: 18 start-page: 337 issue: 5 year: 1994 ident: 13_CR8 publication-title: Cell Biology International doi: 10.1006/cbir.1994.1083 – volume: 327 start-page: 224 issue: 2 year: 1993 ident: 13_CR11 publication-title: FEBS Lett. doi: 10.1016/0014-5793(93)80174-S – volume: 19 start-page: 99 issue: 1 year: 2000 ident: 13_CR28 publication-title: Protein Expr. Purif. doi: 10.1006/prep.2000.1221 – volume: 67 start-page: 31 issue: 1 year: 1988 ident: 13_CR25 publication-title: Gene doi: 10.1016/0378-1119(88)90005-4 – reference: 10722228 - Arch Immunol Ther Exp (Warsz). 2000;48(1):27-30 – reference: 11163360 - FEBS Lett. 2001 Jan 5;487(3):367-71 – reference: 10959046 - Eur J Cancer. 2000 Aug;36(13 Spec No):1595-606 – reference: 10833396 - Protein Expr Purif. 2000 Jun;19(1):99-106 – reference: 7504254 - Prep Biochem. 1993 Nov;23(4):493-515 – reference: 11994743 - Nat Rev Mol Cell Biol. 2002 Apr;3(4):233-45 – reference: 2002030 - J Biol Chem. 1991 Mar 15;266(8):4831-9 – reference: 11964124 - Cell Mol Life Sci. 2002 Mar;59(3):456-62 – reference: 12020799 - Biochim Biophys Acta. 2002 Jun 21;1602(2):114-30 – reference: 10676810 - Cell. 2000 Feb 4;100(3):293-6 – reference: 942051 - Anal Biochem. 1976 May 7;72:248-54 – reference: 11073097 - J Leukoc Biol. 2000 Nov;68(5):603-13 – reference: 11185916 - Tanpakushitsu Kakusan Koso. 2000 Dec;45(16):2624-31 – reference: 7687570 - FEBS Lett. 1993 Jul 26;327(2):224-30 – reference: 11250711 - Breast Cancer Res. 2000;2(3):203-10 – reference: 11389470 - Nat Rev Mol Cell Biol. 2001 Jun;2(6):467-75 – reference: 8672527 - Biochim Biophys Acta. 1996 Jun 7;1287(2-3):121-49 – reference: 9281320 - Arch Biochem Biophys. 1997 Sep 1;345(1):135-42 – reference: 8049678 - Cell Biol Int. 1994 May;18(5):337-44 – reference: 3047011 - Gene. 1988 Jul 15;67(1):31-40 – reference: 11313907 - Oncogene. 2001 Mar 26;20(13):1601-6 – reference: 6172716 - Nature. 1981 Dec 24;294(5843):771-3 – reference: 7537961 - Bioessays. 1995 Apr;17(4):321-30 – reference: 11121839 - Trends Pharmacol Sci. 2000 Dec;21(12):489-95 – reference: 12134161 - Nat Cell Biol. 2002 Aug;4(8):632-8 – reference: 11980666 - Cancer Res. 2002 May 1;62(9):2669-74 – reference: 1280108 - Biochem J. 1992 Nov 1;287 ( Pt 3):985-93 – reference: 9029101 - J Immunol. 1997 Feb 15;158(4):1650-9 |
| SSID | ssj0044076 |
| Score | 1.6966618 |
| Snippet | v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers.... Q556+.5%Q786; v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and... |
| SourceID | pubmedcentral wanfang proquest pubmed crossref chongqing |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 13 |
| SubjectTerms | Bacteria Bacterial Proteins - biosynthesis Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Biotechnology & Food Sciences Glutathione Transferase - biosynthesis Glutathione Transferase - genetics Glutathione Transferase - isolation & purification Kinases Oncogene Protein pp60(v-src) - biosynthesis Oncogene Protein pp60(v-src) - chemistry Oncogene Protein pp60(v-src) - genetics Oncogene Protein pp60(v-src) - isolation & purification Protein Engineering - methods Proteins Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - isolation & purification Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Signal transduction |
| Title | Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system |
| URI | http://lib.cqvip.com/qk/86281A/20061/21490247.html https://www.ncbi.nlm.nih.gov/pubmed/16365920 https://www.proquest.com/docview/883392375 https://www.proquest.com/docview/20730198 https://www.proquest.com/docview/67581505 https://d.wanfangdata.com.cn/periodical/zjdxxbb-e200601003 https://pubmed.ncbi.nlm.nih.gov/PMC1361754 |
| Volume | 7 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagVSUuiHfDQrEEBw51u07sOHtCFO224lAh2kp7i_zsA-Rsd7uw9Nczk2SzrKpyycWOk3jGM9_E428I-SA0xBRg95mX3jB0uUwHnjGrjVZ9nSvr6izf4_zoTHwdy3GbmzNr0yqXNrE21K6y-I98H4viAhhR8tPkmmHRKNxcbStoPCSbyFyGGV1q3MVbAmKV-nBRrjLGZcFbZp884_tXt_OGq3vvAEEQEitcVPH8GtzFuoO6gzrvJk9u_dYx6Hj-j18aPSGPW0BJPzca8JQ88PEZ2WpKTP55Tsrhok11jbt0Mp9iZlAtjF2qo6PmssKTDVhAglaBHp6csjCfeUd_sZOppXj4hGpqGkpneIzvRqMNB_QLcjYann45Ym1RBWaRm52lshgo6yXMjxPcBQl4IgTpnXKpT70M1ujUZk4MUFam4DxkwaSqr9IA4ubZS7IRq-i3CRUyDaLIAnciF9CktS18gIhSgwYoXSSk180qOGX7A6mmyhRiMgAGKiF7y3kubctHjmUxfpYYl4CQShQSlsnMy1pICfnY3TBpqDju79pbCq5s1-Ss7DQoIe-6VlhMuEOio6_qEdDgDYr7e2B8BRgaxnjVqMHqVfIMt6j7CVFrCtJ1QCLv9ZZ4eVETevMMcKQUCXnfqtLqpW-v3GJhTOnTmjkHbPDr_35bjzxa_SR6QzZupnP_FmDTjdmpFwdci9HhDtk8GB5_-_4XDH8bIA |
| linkProvider | ProQuest |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1LT9wwELYoFWovVd9Nty2W2ko9YNg4dpw9VFUfLEuhXACJW3D84NEqWXbZsvCf-h87k9d2heiNsx3H8YxnvonH3xDyTmiIKcDuMyddxtDlMu3DiBmdadXVsTK2zPLdiQf74vuBPFggf5q7MJhW2djE0lDbwuA_8jUsigtgRMlPwzOGRaPwcLWpoFFpxZa7vICIbfxx8xuI9z3n_fW9rwNWFxVgBrnJGZdJTxknQTmsCK2X4E-9l84qyx130ptMcxNZ0cO5ZkkY-shnXHUV9_C5YQTj3iF3BcwGN1TS32gMv4DYqLzMFKuIhTIJayahOArXTq8mFTf46hcEXUjkcFzkR2fgnuYd4jWUez1Zc-lC517nR__4wf5D8qAGsPRzpXGPyILLH5OlqqTl5ROSrk_r1Np8hQ4nI8xEKoW_QnVuaXZS4E0KLFhBC083dveYn4ydpb_Z7shQvOxCNc0qCml4jWtHoxXn9FOyfyvr_Yws5kXuXhAqJPciiXxoRSygSWuTOA8RrAaNUzoJSKddVQAB5idSW6UcYkAAIiogq806p6bmP8cyHL9SjINASCkKCctyxmkppIB8aB8YVtQfN3ftNIJLaxswTluNDchy2wqbF09kdO6KcgQ0sL3k5h4YzwFmhzGeV2owm0oc4ZF4NyBqTkHaDkgcPt-SnxyXBOJhBLhVioC8rVVpNumrUzudZlnqeMnUAzb_5X-_bZncG-z92E63N3e2OuT-7AfVK7J4Ppq41wDZzrM35Uah5PC2d-ZfElJWNg |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Expression%2C+purification%2C+and+bioactivity+of+GST-fused+v-Src+from+a+bacterial+expression+system&rft.jtitle=Journal+of+Zhejiang+University.+B.+Science&rft.au=Gong%2C+Xing-Guo&rft.au=Ji%2C+Jing&rft.au=Xie%2C+Jie&rft.au=Zhou%2C+Yuan&rft.date=2006&rft.issn=1673-1581&rft.volume=7&rft.issue=1&rft.spage=13&rft_id=info:doi/10.1631%2Fjzus.2006.B0013&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F86281A%2F86281A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fzjdxxbb-e%2Fzjdxxbb-e.jpg |