Large-scale isolation and purification of C-phycocyanin from the cyanobacteria Anabaena marina using expanded bed adsorption chromatography

• Published in: Journal of chemical technology and biotechnology (1986) Vol. 85; no. 6; pp. 783 - 792
• Main Authors: Ramos, Amparo, Acién, F.Gabriel, Fernández-Sevilla, José M, González, Cynthia V, Bermejo, Ruperto
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.06.2010; Wiley
• Subjects: Adsorption; Anabaena marina; anion-exchange chromatography; Applied sciences; C-phycocyanin; Chemical engineering; Chromatography; Columns (process); Cost engineering; Exact sciences and technology; expanded bed adsorption chromatography; Extraction; Ion exchange; Macromolecules; Marinas; phycobiliproteins; preparative protein purification; Purification; Viscosity; Anabaena marina; Purification; Scale effect; Biomass; Adsorption chromatography; preparative protein purification; Ion exchange chromatography; Extrapolation; expanded bed adsorption chromatography; Adsorption; C-phycocyanin; Ion exchange; anion-exchange chromatography; phycobiliproteins

BACKGROUND: Phycobiliproteins are water soluble proteins useful as fluorescent markers of cells and macromolecules, and as natural colorants, and are anticarcinogenic. Although phycobiliproteins have many applications, their use is limited by the high cost of the purified macromolecules, mainly rela...