Expression, purification and osteogenic bioactivity of recombinant human BMP-4, -9, -10, -11 and -14

• Published in: Protein expression and purification Vol. 63; no. 2; pp. 89 - 94
• Main Authors: Bessa, P.C., Cerqueira, M.T., Rada, T., Gomes, M.E., Neves, N.M., Nobre, A., Reis, R.L., Casal, M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2009
• Subjects: Adult Stem Cells - drug effects; Adult Stem Cells - metabolism; Animals; Biomarkers - metabolism; Bone morphogenetic protein-10; Bone morphogenetic protein-4; Bone morphogenetic protein-9; Bone Morphogenetic Proteins - biosynthesis; Bone Morphogenetic Proteins - isolation & purification; Bone Morphogenetic Proteins - pharmacology; C2C12; Cell Line; Escherichia coli; Gene Expression - drug effects; Growth differentiation factor-11; Growth differentiation factor-5; Humans; Mice; Osteogenesis; Recombinant Proteins - biosynthesis; Recombinant Proteins - isolation & purification; Recombinant Proteins - pharmacology; Bone morphogenetic protein-10; C2C12; Bone morphogenetic protein-9; Growth differentiation factor-5; Growth differentiation factor-11; Bone morphogenetic protein-4
• ISSN: 1046-5928; 1096-0279
• DOI: 10.1016/j.pep.2008.09.014

Bone morphogenetic proteins (BMPs) are cytokines from the TGF-β superfamily, with important roles during embryonic development and in the induction of bone and cartilage tissue differentiation in the adult body. In this contribution, we report the expression of recombinant human BMP-4, BMP-9, BMP-10, BMP-11 (or growth differentiation factor-11, GDF-11) and BMP-14 (GDF-5), using Escherichia coli pET-25b vector. BMPs were overexpressed, purified by affinity his-tag chromatography and shown to induce the expression of early markers of bone differentiation (e.g. smad-1, smad-5, runx2/cbfa1, dlx5, osterix, osteopontin, bone sialoprotein and alkaline phosphatase) in C2C12 cells and in human adipose stem cells. The described approach is a promising method for producing large amounts of different recombinant BMPs that show potential for novel biomedical applications.