Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast

• Published in: Nature biotechnology Vol. 16; no. 13; pp. 1334 - 1337
• Main Authors: Klein, Christine, Paul, Jeremy I, Sauvé, Karen, Schmidt, Mary M, Arcangeli, Loretta, Ransom, John, Trueheart, Joshua, Manfredi, John P, Broach, James R, Murphy, Andrew J
• Format: Journal Article
• Language: English
• Published: New York, NY Nature 01.12.1998
• Subjects: Amino Acid Sequence; Biological and medical sciences; Cell Line; Diverse techniques; Fundamental and applied biological sciences. Psychology; Humans; Ligands; Molecular and cellular biology; Molecular Sequence Data; Monocytes - metabolism; N-Formylmethionine Leucyl-Phenylalanine - metabolism; Neutrophils - metabolism; Peptides - chemistry; Peptides - metabolism; Receptors, Formyl Peptide; Receptors, Immunologic - agonists; Receptors, Immunologic - metabolism; Receptors, Peptide - agonists; Receptors, Peptide - metabolism; Recombinant Proteins - agonists; Recombinant Proteins - metabolism; Saccharomyces cerevisiae - genetics; Fungi; Recombinant microorganism; Signal transduction; Agonist; Yeast; Ascomycetes; Pharmacology; Method; Saccharomyces cerevisiae; Orphan receptor; Thallophyta; G protein

We describe a procedure for isolating agonists for mammalian G protein-coupled receptors of unknown function. Human formyl peptide receptor like-1 (FPRL-1) receptor, originally identified as an orphan G protein-coupled receptor related to the formyl peptide receptor (FPR1), was expressed in Saccharo...