Quantitation of IgG and IgM Human Anti-Mouse Antibodies (HAMA) Interference in CA 125 Measurements Using Affinity Chromatography

• Published in: Clinical chemistry and laboratory medicine Vol. 36; no. 1; pp. 23 - 28
• Main Authors: Koper, Norbert P., Thomas, Chris M. G., Massuger, Leon F. A. G., Segers, Martin F. G., Olthaar, André J., Verbeek, André L. M.
• Format: Journal Article
• Language: English
• Published: Berlin Walter de Gruyter 01.01.1998; New York, NY
• Subjects: Animals; Antibodies, Heterophile - blood; Antibodies, Monoclonal; Biological and medical sciences; CA-125 Antigen - blood; CA-125 Antigen - immunology; Chromatography, Affinity; Humans; Immunoglobulin G - blood; Immunoglobulin G - isolation & purification; Immunoglobulin M - blood; Immunoglobulin M - isolation & purification; Immunological methods for diagnosis and exploration; Immunopathology; Immunoradiometric Assay; Immunosorbents - metabolism; Indium Radioisotopes; Medical sciences; Mice; Radioimmunodetection; Reagent Kits, Diagnostic; Serological tests; Performance evaluation; Human; Biological fluid; Immunization; Immunoaffinity chromatography; Interference; Biological marker; Malignant tumor; Female genital diseases; Immunological method; Ovarian diseases; Ovary; Investigation method; Clinical biology; CA 125 antigen; Serum; Quantitative analysis

Currently no available immunoassay system offers complete protection against spuriously elevated or lowered results due to interference by Human Anti-Mouse Antibodies (HAMA). Although routine use of chromatography procedures is not an acceptable option because of the extra cost and workload involved...