CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newl...
Saved in:
Published in | Metabolic engineering Vol. 38; pp. 293 - 302 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Belgium
Elsevier Inc
01.11.2016
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newly developed RNA-guided genome editing system, yet its application for cyanobacteria engineering has yet to be reported. Here we demonstrated that CRISPR-Cas9 system can effectively trigger programmable double strand break (DSB) at the chromosome of PCC 7942 and provoke cell death. With the co-transformation of template plasmid harboring the gene cassette and flanking homology arms, CRISPR-Cas9-mediated DSB enabled precise gene integration, ameliorated the homologous recombination efficiency and allowed the use of lower amount of template DNA and shorter homology arms. The CRISPR-Cas9-induced cell death imposed selective pressure and enhanced the chance of concomitant integration of gene cassettes into all chromosomes of PCC 7942, hence accelerating the process of obtaining homogeneous and stable recombinant strains. We further explored the feasibility of engineering cyanobacteria by CRISPR-Cas9-assisted simultaneous glgc knock-out and gltA/ppc knock-in, which improved the succinate titer to 435.0±35.0μg/L, an ≈11-fold increase when compared with that of the wild-type cells. These data altogether justify the use of CRISPR-Cas9 for genome engineering and manipulation of metabolic pathways in cyanobacteria.
•CRISPR-Cas9 triggers programmable double strand break (DSB) and death in cyanobacteria PCC 7942.•CRISPR-Cas9-mediated DSB enables precise gene integration in PCC 7942.•CRISPR-Cas9-induced DSB improves homologous recombination efficiency.•CRISPR-Cas9 accelerates the process of obtaining homogenous recombinant strain.•CRISPR-Cas9 enables metabolic engineering of PCC 7942 for succinate production. |
---|---|
AbstractList | Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newly developed RNA-guided genome editing system, yet its application for cyanobacteria engineering has yet to be reported. Here we demonstrated that CRISPR-Cas9 system can effectively trigger programmable double strand break (DSB) at the chromosome of PCC 7942 and provoke cell death. With the co-transformation of template plasmid harboring the gene cassette and flanking homology arms, CRISPR-Cas9-mediated DSB enabled precise gene integration, ameliorated the homologous recombination efficiency and allowed the use of lower amount of template DNA and shorter homology arms. The CRISPR-Cas9-induced cell death imposed selective pressure and enhanced the chance of concomitant integration of gene cassettes into all chromosomes of PCC 7942, hence accelerating the process of obtaining homogeneous and stable recombinant strains. We further explored the feasibility of engineering cyanobacteria by CRISPR-Cas9-assisted simultaneous glgc knock-out and gltA/ppc knock-in, which improved the succinate titer to 435.0±35.0μg/L, an ≈11-fold increase when compared with that of the wild-type cells. These data altogether justify the use of CRISPR-Cas9 for genome engineering and manipulation of metabolic pathways in cyanobacteria.
•CRISPR-Cas9 triggers programmable double strand break (DSB) and death in cyanobacteria PCC 7942.•CRISPR-Cas9-mediated DSB enables precise gene integration in PCC 7942.•CRISPR-Cas9-induced DSB improves homologous recombination efficiency.•CRISPR-Cas9 accelerates the process of obtaining homogenous recombinant strain.•CRISPR-Cas9 enables metabolic engineering of PCC 7942 for succinate production. Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus PCC 7942 poses challenges because of their oligoploidy nature and long-term instability of the introduced gene. CRISPR-Cas9 is a newly developed RNA-guided genome editing system, yet its application for cyanobacteria engineering has yet to be reported. Here we demonstrated that CRISPR-Cas9 system can effectively trigger programmable double strand break (DSB) at the chromosome of PCC 7942 and provoke cell death. With the co-transformation of template plasmid harboring the gene cassette and flanking homology arms, CRISPR-Cas9-mediated DSB enabled precise gene integration, ameliorated the homologous recombination efficiency and allowed the use of lower amount of template DNA and shorter homology arms. The CRISPR-Cas9-induced cell death imposed selective pressure and enhanced the chance of concomitant integration of gene cassettes into all chromosomes of PCC 7942, hence accelerating the process of obtaining homogeneous and stable recombinant strains. We further explored the feasibility of engineering cyanobacteria by CRISPR-Cas9-assisted simultaneous glgc knock-out and gltA/ppc knock-in, which improved the succinate titer to 435.0±35.0μg/L, an ≈11-fold increase when compared with that of the wild-type cells. These data altogether justify the use of CRISPR-Cas9 for genome engineering and manipulation of metabolic pathways in cyanobacteria. |
Author | Shen, Claire R. Sung, Li-Yu Hu, Yu-Chen Huang, Chun-Hung Li, Hung Wu, Meng-Ying |
Author_xml | – sequence: 1 givenname: Hung surname: Li fullname: Li, Hung – sequence: 2 givenname: Claire R. surname: Shen fullname: Shen, Claire R. – sequence: 3 givenname: Chun-Hung surname: Huang fullname: Huang, Chun-Hung – sequence: 4 givenname: Li-Yu surname: Sung fullname: Sung, Li-Yu – sequence: 5 givenname: Meng-Ying surname: Wu fullname: Wu, Meng-Ying – sequence: 6 givenname: Yu-Chen surname: Hu fullname: Hu, Yu-Chen email: yuchen@che.nthu.edu.tw |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/27693320$$D View this record in MEDLINE/PubMed |
BookMark | eNp9kEtr3DAUhUVJaR7tLygULbOxq2u9RosuwpAmgUBD2q6FLF1PNYylVLID8-_rZJIsu7oPzrmH-52So5QTEvIZWAsM1Ndtux97TG23DC0zLWPqHTkBZlSjYSWO3nqtjslprVvGAKSBD-S408pw3rETcre-v_l5d9-sXTV0yIVOf5BuMOURKaZNTIglpg3NA_V7l3Lv_LRsHHUp0Dp7H5ObkD6UHGY_xZw-kveD21X89FLPyO_vl7_W183tj6ub9cVt40Unp2bFBwCBSqqge2cMC0wOukMRmFYdCC9h8CCF9t5rLzXHIFH13UoowQEUPyPnh7tL9N8Z62THWD3udi5hnquFFZdcGKn4IuUHqS-51oKDfShxdGVvgdknlHZrn1HaJ5SWGbugXFxfXgLmfsTw5nlltwi-HQS4vPkYsdjqIyaPIRb0kw05_jfgHweQhoI |
CitedBy_id | crossref_primary_10_1080_21655979_2019_1682108 crossref_primary_10_1016_j_greenca_2024_05_002 crossref_primary_10_1002_btpr_3008 crossref_primary_10_1016_j_copbio_2017_03_014 crossref_primary_10_1021_acssynbio_0c00006 crossref_primary_10_3390_microorganisms11020455 crossref_primary_10_1016_j_algal_2023_103068 crossref_primary_10_1073_pnas_1618596114 crossref_primary_10_1093_jxb_erac053 crossref_primary_10_1021_acssynbio_0c00091 crossref_primary_10_1186_s13068_023_02385_1 crossref_primary_10_1016_j_jcou_2018_10_008 crossref_primary_10_1016_j_ymben_2020_10_004 crossref_primary_10_1016_j_biotechadv_2020_107554 crossref_primary_10_1002_bit_26915 crossref_primary_10_1016_j_copbio_2018_01_002 crossref_primary_10_1371_journal_pone_0225375 crossref_primary_10_1021_acs_jafc_1c04632 crossref_primary_10_3389_fmicb_2022_994365 crossref_primary_10_1021_acssynbio_9b00066 crossref_primary_10_1515_psr_2022_0160 crossref_primary_10_1186_s12934_016_0595_3 crossref_primary_10_1016_j_biotechadv_2019_107433 crossref_primary_10_1093_jimb_kuab075 crossref_primary_10_2174_1389203724666230110163234 crossref_primary_10_3389_fbioe_2022_913820 crossref_primary_10_1007_s00253_019_09726_x crossref_primary_10_1186_s13068_020_1653_y crossref_primary_10_3390_metabo10060250 crossref_primary_10_1186_s13068_020_01727_7 crossref_primary_10_1002_jobm_202100417 crossref_primary_10_1016_j_synbio_2018_10_003 crossref_primary_10_3389_fpls_2020_00279 crossref_primary_10_1016_j_copbio_2019_05_012 crossref_primary_10_1002_biot_201800496 crossref_primary_10_1016_j_procbio_2021_06_006 crossref_primary_10_1016_j_enzmictec_2020_109619 crossref_primary_10_1016_j_copbio_2022_102698 crossref_primary_10_1016_j_biortech_2022_128104 crossref_primary_10_1016_j_ymben_2018_05_013 crossref_primary_10_1016_j_biortech_2019_121932 crossref_primary_10_1007_s12257_019_0447_1 crossref_primary_10_1016_j_ymben_2018_03_014 crossref_primary_10_1186_s12934_019_1255_1 crossref_primary_10_1186_s12951_023_02139_z crossref_primary_10_3390_ijms22073327 crossref_primary_10_1016_j_mec_2019_e00108 crossref_primary_10_1007_s12033_022_00501_4 crossref_primary_10_3389_fbioe_2019_00459 crossref_primary_10_1021_acssuschemeng_3c05735 crossref_primary_10_1021_acssynbio_8b00437 crossref_primary_10_1111_ppl_13455 crossref_primary_10_1007_s12010_023_04708_2 crossref_primary_10_1016_j_tibtech_2022_09_002 crossref_primary_10_1002_bab_2069 crossref_primary_10_1007_s00253_018_9221_0 crossref_primary_10_1002_bbb_2063 crossref_primary_10_1016_j_tibtech_2018_05_011 crossref_primary_10_1016_j_heliyon_2023_e14708 crossref_primary_10_1021_acssynbio_2c00375 crossref_primary_10_1186_s12934_019_1109_x crossref_primary_10_1021_acssynbio_2c00653 crossref_primary_10_1007_s10295_017_2000_6 crossref_primary_10_1016_j_synbio_2018_09_004 crossref_primary_10_1016_j_copbio_2017_12_023 crossref_primary_10_1021_acs_energyfuels_2c02922 crossref_primary_10_1016_j_mimet_2021_106315 crossref_primary_10_3389_fmicb_2016_01809 crossref_primary_10_3390_life12122013 crossref_primary_10_1111_tpj_16074 crossref_primary_10_1016_j_ymben_2022_11_005 crossref_primary_10_1080_21655979_2020_1837458 crossref_primary_10_1016_j_jbiotec_2022_10_011 crossref_primary_10_1186_s13068_017_0957_z crossref_primary_10_1016_j_synbio_2020_08_001 crossref_primary_10_1093_nar_gky1165 crossref_primary_10_3389_fbioe_2022_897204 crossref_primary_10_1007_s11157_022_09618_z crossref_primary_10_1002_biot_201700586 crossref_primary_10_1002_bit_26727 crossref_primary_10_2174_1872208315666210419102117 crossref_primary_10_1016_j_biotechadv_2018_04_007 crossref_primary_10_1016_j_algal_2022_102696 crossref_primary_10_1021_acs_est_9b04218 crossref_primary_10_1002_biot_201700591 crossref_primary_10_1016_j_biotechadv_2019_04_005 crossref_primary_10_3390_life9030054 crossref_primary_10_1016_j_rser_2023_113958 crossref_primary_10_1021_acssynbio_1c00252 crossref_primary_10_1016_j_mtbio_2023_100583 crossref_primary_10_3390_ijms21197204 crossref_primary_10_1080_07388551_2023_2166455 crossref_primary_10_1002_biot_202100507 crossref_primary_10_1016_j_biortech_2019_122135 crossref_primary_10_1016_j_biortech_2022_128350 crossref_primary_10_1016_j_fbio_2019_01_003 crossref_primary_10_1007_s11274_021_03157_5 crossref_primary_10_1021_acssynbio_9b00417 crossref_primary_10_1016_j_ymben_2018_05_007 crossref_primary_10_1002_biot_201700596 crossref_primary_10_1016_j_algal_2020_102158 crossref_primary_10_1007_s10295_019_02195_1 crossref_primary_10_15237_gida_GD23120 crossref_primary_10_1186_s12934_021_01529_y crossref_primary_10_1002_biot_201900228 crossref_primary_10_1093_nar_gky572 crossref_primary_10_1007_s00253_018_9046_x crossref_primary_10_1093_nar_gkab791 crossref_primary_10_1080_1040841X_2020_1772195 crossref_primary_10_1021_acssynbio_0c00106 crossref_primary_10_1093_plphys_kiae261 crossref_primary_10_1016_j_mimet_2019_105686 crossref_primary_10_1016_j_biotechadv_2019_107447 crossref_primary_10_1002_bit_26747 crossref_primary_10_1021_acssynbio_7b00020 crossref_primary_10_1016_j_algal_2023_103088 crossref_primary_10_3389_fbioe_2020_00513 crossref_primary_10_1360_TB_2022_1210 crossref_primary_10_1051_bioconf_20236101009 crossref_primary_10_3390_bioengineering5040111 crossref_primary_10_1111_raq_12322 crossref_primary_10_3390_microorganisms7100409 crossref_primary_10_3390_life12101537 crossref_primary_10_1007_s00425_018_3047_y crossref_primary_10_1039_C8NP00089A crossref_primary_10_1007_s00253_017_8489_9 crossref_primary_10_1007_s11274_024_04064_1 crossref_primary_10_1021_acssynbio_7b00251 crossref_primary_10_3390_en12183515 crossref_primary_10_1016_j_fuel_2022_123330 crossref_primary_10_3389_fbioe_2019_00033 crossref_primary_10_1002_biot_201600644 crossref_primary_10_3390_genes9110520 crossref_primary_10_3390_en17020408 crossref_primary_10_3390_microorganisms8121849 crossref_primary_10_1186_s12934_022_01830_4 |
Cites_doi | 10.1128/AEM.04023-14 10.1038/nbt.1586 10.1111/j.1574-6968.2011.02368.x 10.1016/j.gene.2012.11.016 10.1016/j.jbiotec.2015.02.005 10.1021/sb500351f 10.1016/j.ymben.2015.06.006 10.1038/mt.2015.220 10.1016/j.tibtech.2016.02.004 10.1038/mt.2015.218 10.1016/j.copbio.2014.09.007 10.1038/mt.2015.192 10.1002/bit.24509 10.1038/nbt.3026 10.1002/bit.25490 10.1128/AEM.01248-15 10.1016/j.tibtech.2015.03.009 10.1371/journal.pone.0047837 10.1016/j.jbiotec.2014.04.029 10.1016/j.ymben.2014.01.001 10.1099/mic.0.062950-0 10.1021/acssynbio.5b00057 10.1099/00221287-147-8-2077 10.1073/pnas.1200074109 10.1128/AEM.03326-12 10.3389/fmicb.2013.00246 10.1186/s12934-015-0405-3 10.1016/j.ymben.2015.03.019 10.1016/j.mimet.2004.12.007 10.1016/S0168-1605(99)00132-4 10.1016/j.cell.2015.09.038 10.1126/science.1225829 10.1128/AEM.00202-10 10.1128/AEM.00397-08 10.1039/c2ee23148d 10.1016/j.ymben.2015.08.002 10.1128/JB.183.5.1740-1747.2001 10.1016/j.ymben.2015.03.013 10.1038/nprot.2013.132 10.1021/acssynbio.5b00043 10.1016/j.cell.2014.05.010 10.1093/nar/gkt135 10.1099/mic.0.28030-0 10.1093/nar/gkw159 10.1038/nature14299 10.1126/science.1231143 10.1093/nar/gku623 10.1016/j.ymben.2013.09.007 10.1186/s12934-016-0514-7 10.1126/science.1232033 10.1111/j.1365-2958.2011.07600.x 10.1038/mt.2016.10 10.1371/journal.pone.0056470 10.1073/pnas.1213024110 10.1016/j.cell.2013.02.022 10.1038/nbt.2508 10.1038/srep04489 10.1128/JB.01016-10 10.1039/c2ee23181f 10.1038/nbt.2808 10.1093/abbs/gmv007 10.1038/nmeth.3580 10.1016/j.cell.2013.08.021 |
ContentType | Journal Article |
Copyright | 2016 International Metabolic Engineering Society Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
Copyright_xml | – notice: 2016 International Metabolic Engineering Society – notice: Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
DOI | 10.1016/j.ymben.2016.09.006 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering Biology |
EISSN | 1096-7184 |
EndPage | 302 |
ExternalDocumentID | 10_1016_j_ymben_2016_09_006 27693320 S1096717616301458 |
Genre | Journal Article |
GroupedDBID | --- --K --M .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 4.4 457 4G. 53G 5VS 7-5 71M 8P~ AAAJQ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABFNM ABGSF ABJNI ABMAC ABNUV ABUDA ABXDB ABYKQ ACDAQ ACGFS ACRLP ADBBV ADEWK ADEZE ADFGL ADMUD ADUVX AEBSH AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGEKW AGHFR AGRDE AGUBO AGYEJ AHPOS AIEXJ AIKHN AITUG AJBFU AJOXV AKURH ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG CJTIS COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD ENUVR EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-Q GBLVA HVGLF HZ~ IHE J1W KOM LG5 LUGTX M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SDF SDG SDP SES SEW SPC SPCBC SSG SSI SSU SSZ T5K UHS XPP ZMT ZU3 ~G- AAHBH AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 |
ID | FETCH-LOGICAL-c425t-83f114e656d7ba990d05f72e4d076214c51fc1547ccc7c573ed5e6b2846431163 |
IEDL.DBID | AIKHN |
ISSN | 1096-7176 |
IngestDate | Sat Aug 17 02:53:11 EDT 2024 Thu Sep 26 16:19:57 EDT 2024 Sat Sep 28 08:50:00 EDT 2024 Fri Feb 23 02:37:47 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Keywords | PCC 7942 CRISPR-Cas9 Succinate Cyanobacteria Metabolic engineering Genome editing Synechococcus elongatus |
Language | English |
License | Copyright © 2016 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c425t-83f114e656d7ba990d05f72e4d076214c51fc1547ccc7c573ed5e6b2846431163 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 27693320 |
PQID | 1835349563 |
PQPubID | 23479 |
PageCount | 10 |
ParticipantIDs | proquest_miscellaneous_1835349563 crossref_primary_10_1016_j_ymben_2016_09_006 pubmed_primary_27693320 elsevier_sciencedirect_doi_10_1016_j_ymben_2016_09_006 |
PublicationCentury | 2000 |
PublicationDate | November 2016 2016-Nov 2016-11-00 20161101 |
PublicationDateYYYYMMDD | 2016-11-01 |
PublicationDate_xml | – month: 11 year: 2016 text: November 2016 |
PublicationDecade | 2010 |
PublicationPlace | Belgium |
PublicationPlace_xml | – name: Belgium |
PublicationTitle | Metabolic engineering |
PublicationTitleAlternate | Metab Eng |
PublicationYear | 2016 |
Publisher | Elsevier Inc |
Publisher_xml | – name: Elsevier Inc |
References | Jiang, Bikard, Cox, Zhang, Marraffini (bib23) 2013; 31 Hsu Patrick, Lander Eric, Zhang (bib21) 2014; 157 Oliver, Machado, Yoneda, Atsumi (bib46) 2014; 22 Ramey, Baron-Sola, Aucoin, Boyle (bib49) 2015; 4 Griese, Lange, Soppa (bib18) 2011; 323 Ran, Cong, Yan, Scott, Gootenberg, Kriz, Zetsche, Shalem, Wu, Makarova, Koonin, Sharp, Zhang (bib50) 2015; 520 Lange, Zerulla, Breuert, Soppa (bib30) 2011; 80 Pyne, Moo-Young, Chung, Chou (bib47) 2015; 81 Larson, Gilbert, Wang, Lim, Weissman, Qi (bib31) 2013; 8 Ran, Hsu Patrick, Lin, Gootenberg Jonathan, Konermann, Trevino, Scott David, Inoue, Matoba, Zhang, Zhang (bib51) 2013; 154 Baroukh, Munoz-Tamayo, Steyer, Bernard (bib4) 2015; 30 Kiani, Chavez, Tuttle, Hall, Chari, Ter-Ovanesyan, Qian, Pruitt, Beal, Vora, Buchthal, Kowal, Ebrahimkhani, Collins, Weiss, Church (bib26) 2015; 12 Cobb, Wang, Zhao (bib11) 2015; 4 Zhou, Zhu, Cai, Li (bib65) 2016; 15 Atsumi, Higashide, Liao (bib2) 2009; 27 Doench, Hartenian, Graham, Tothova, Hegde, Smith, Sullender, Ebert, Xavier, Root (bib15) 2014; 32 Mali, Yang, Esvelt, Aach, Guell, DiCarlo, Norville, Church (bib36) 2013; 339 Carrieri, Paddock, Maness, Seibert, Yu (bib7) 2012; 5 Fu, Sander, Reyon, Cascio, Joung (bib16) 2014; 32 Angermayr, Gorchs Rovira, Hellingwerf (bib1) 2015; 33 Cong, Ran, Cox, Lin, Barretto, Habib, Hsu, Wu, Jiang, Marraffini, Zhang (bib12) 2013; 339 Ditty, Canales, Anderson, Williams, Golden (bib14) 2005; 151 Kusakabe, Tatsuke, Tsuruno, Hirokawa, Atsumi, Liao, Hanai (bib27) 2013; 20 Oliver, Machado, Yoneda, Atsumi (bib45) 2013; 110 Turan, Zehe, Kuehle, Qiao, Bode (bib57) 2013; 515 Berla, Saha, Immethun, Maranas, Moon, Pakrasi (bib5) 2013; 4 Chen, Hu, Almeida, Liu, Balakrishnan, Covill-Cooke, Lim, Huang (bib9) 2016 Ma, Chang, Wang, Liu, Zhang, Lu, Gao, Shi, Zhao, Xia (bib34) 2014; 4 Shen, Liao (bib55) 2012; 5 Bischoff, Luthy, Altwegg, Baggi (bib6) 2005; 61 Uyttendaele, van Boxstael, Debevere (bib58) 1999; 52 Mougiakos, Bosma, de Vos, van Kranenburg, van der Oost (bib39) 2016; 34 Xu, Park, Zhao, Xu, El Refaey, Gao, Zhu, Ma, Han (bib63) 2016; 24 Scholz, Lange, Hein, Hess, Backofen (bib54) 2013; 8 Bakondi, Lv, Lu, Jones, Tsai, Kim, Levy, Akhtar, Breunig, Svendsen, Wang (bib3) 2016; 24 Lan, Chuang, Shen, Lee, Ro, Liao (bib28) 2015; 31 Nair, Thomas, Golden (bib41) 2001; 183 Li, Lin, Huang, Zhang, Wang, Tang Y-j, Chen, Zhao (bib32) 2015; 31 Matsuoka, Takahama, Ogawa (bib37) 2001; 147 Suzuki, Ohkawa, Moriya, Matsubara, Nagaike, Iwasaki, Fujiwara, Tsuzuki, Nakamura (bib56) 2010; 76 DiCarlo, Norville, Mali, Rios, Aach, Church (bib13) 2013; 41 Qi, Larson, Gilbert, Doudna, Weissman, Arkin, Lim (bib48) 2013; 152 Lan, Liao (bib29) 2012; 109 Savakis, Hellingwerf (bib53) 2015; 33 Huang, Zheng, Jiang, Hu, Lu (bib22) 2015; 47 Wang, Zhang, Seo, Choi, Lu, Jin, Blaschek (bib61) 2015; 200 Wendt, Ungerer, Cobb, Zhao, Pakrasi (bib62) 2016; 15 Niederholtmeyer, Wolfstadter, Savage, Silver, Way (bib42) 2010; 76 Ruffing, Jones (bib52) 2012; 109 Hildenbrand, Stock, Lange, Rother, Soppa (bib20) 2011; 193 Chen, Afonso, Silver, Savage (bib8) 2012; 7 Nozzi, Atsumi (bib43) 2015; 4 Chung, Yeh, Sung, Wu, Chao, Ng, Hu (bib10) 2016 Gründel, Scheunemann, Lockau, Zilliges (bib17) 2012; 158 Jiang, Chen, Duan, Sun, Yang, Yang (bib24) 2015; 81 Lv, Ren, Chen, Wu, Chen (bib33) 2015; 29 Vojta, Dobrinic, Tadic, Bockor, Korac, Julg, Klasic, Zoldos (bib60) 2016; 44 He, Proudfoot, Mileham, McLaren, Whitelaw, Lillico (bib19) 2015; 112 Maeder, Gersbach (bib35) 2016; 24 Muller, Lee, Gasiunas, Davis, Cradick, Siksnys, Bao, Cathomen, Mussolino (bib40) 2016; 24 Oh, van Pijkeren (bib44) 2014; 42 Zetsche, Gootenberg Jonathan, Abudayyeh Omar, Slaymaker Ian, Makarova Kira, Essletzbichler, Volz Sara, Joung, van der Oost, Regev, Koonin Eugene, Zhang (bib64) 2015; 163 van der Woude, Angermayr, Puthan Veetil, Osnato, Hellingwerf (bib59) 2014; 184 Jinek, Chylinski, Fonfara, Hauer, Doudna, Charpentier (bib25) 2012; 337 McEwen, Machado, Connor, Atsumi (bib38) 2013; 79 Uyttendaele (10.1016/j.ymben.2016.09.006_bib58) 1999; 52 He (10.1016/j.ymben.2016.09.006_bib19) 2015; 112 Xu (10.1016/j.ymben.2016.09.006_bib63) 2016; 24 Muller (10.1016/j.ymben.2016.09.006_bib40) 2016; 24 Kusakabe (10.1016/j.ymben.2016.09.006_bib27) 2013; 20 Maeder (10.1016/j.ymben.2016.09.006_bib35) 2016; 24 Hsu Patrick (10.1016/j.ymben.2016.09.006_bib21) 2014; 157 Ma (10.1016/j.ymben.2016.09.006_bib34) 2014; 4 Bakondi (10.1016/j.ymben.2016.09.006_bib3) 2016; 24 Chung (10.1016/j.ymben.2016.09.006_bib10) 2016 Oliver (10.1016/j.ymben.2016.09.006_bib46) 2014; 22 Huang (10.1016/j.ymben.2016.09.006_bib22) 2015; 47 Li (10.1016/j.ymben.2016.09.006_bib32) 2015; 31 Kiani (10.1016/j.ymben.2016.09.006_bib26) 2015; 12 Wendt (10.1016/j.ymben.2016.09.006_bib62) 2016; 15 Suzuki (10.1016/j.ymben.2016.09.006_bib56) 2010; 76 Hildenbrand (10.1016/j.ymben.2016.09.006_bib20) 2011; 193 DiCarlo (10.1016/j.ymben.2016.09.006_bib13) 2013; 41 Jiang (10.1016/j.ymben.2016.09.006_bib23) 2013; 31 Lan (10.1016/j.ymben.2016.09.006_bib29) 2012; 109 Fu (10.1016/j.ymben.2016.09.006_bib16) 2014; 32 Wang (10.1016/j.ymben.2016.09.006_bib61) 2015; 200 Lv (10.1016/j.ymben.2016.09.006_bib33) 2015; 29 Berla (10.1016/j.ymben.2016.09.006_bib5) 2013; 4 Ditty (10.1016/j.ymben.2016.09.006_bib14) 2005; 151 Jiang (10.1016/j.ymben.2016.09.006_bib24) 2015; 81 Bischoff (10.1016/j.ymben.2016.09.006_bib6) 2005; 61 Baroukh (10.1016/j.ymben.2016.09.006_bib4) 2015; 30 Savakis (10.1016/j.ymben.2016.09.006_bib53) 2015; 33 Mali (10.1016/j.ymben.2016.09.006_bib36) 2013; 339 Zetsche (10.1016/j.ymben.2016.09.006_bib64) 2015; 163 Angermayr (10.1016/j.ymben.2016.09.006_bib1) 2015; 33 Carrieri (10.1016/j.ymben.2016.09.006_bib7) 2012; 5 Nozzi (10.1016/j.ymben.2016.09.006_bib43) 2015; 4 Qi (10.1016/j.ymben.2016.09.006_bib48) 2013; 152 McEwen (10.1016/j.ymben.2016.09.006_bib38) 2013; 79 Ran (10.1016/j.ymben.2016.09.006_bib50) 2015; 520 Cobb (10.1016/j.ymben.2016.09.006_bib11) 2015; 4 Griese (10.1016/j.ymben.2016.09.006_bib18) 2011; 323 Oliver (10.1016/j.ymben.2016.09.006_bib45) 2013; 110 Cong (10.1016/j.ymben.2016.09.006_bib12) 2013; 339 Lan (10.1016/j.ymben.2016.09.006_bib28) 2015; 31 Zhou (10.1016/j.ymben.2016.09.006_bib65) 2016; 15 Mougiakos (10.1016/j.ymben.2016.09.006_bib39) 2016; 34 Atsumi (10.1016/j.ymben.2016.09.006_bib2) 2009; 27 Jinek (10.1016/j.ymben.2016.09.006_bib25) 2012; 337 Matsuoka (10.1016/j.ymben.2016.09.006_bib37) 2001; 147 Oh (10.1016/j.ymben.2016.09.006_bib44) 2014; 42 Ramey (10.1016/j.ymben.2016.09.006_bib49) 2015; 4 Scholz (10.1016/j.ymben.2016.09.006_bib54) 2013; 8 Doench (10.1016/j.ymben.2016.09.006_bib15) 2014; 32 Niederholtmeyer (10.1016/j.ymben.2016.09.006_bib42) 2010; 76 Lange (10.1016/j.ymben.2016.09.006_bib30) 2011; 80 Nair (10.1016/j.ymben.2016.09.006_bib41) 2001; 183 Ran (10.1016/j.ymben.2016.09.006_bib51) 2013; 154 Chen (10.1016/j.ymben.2016.09.006_bib9) 2016 Vojta (10.1016/j.ymben.2016.09.006_bib60) 2016; 44 Chen (10.1016/j.ymben.2016.09.006_bib8) 2012; 7 Larson (10.1016/j.ymben.2016.09.006_bib31) 2013; 8 van der Woude (10.1016/j.ymben.2016.09.006_bib59) 2014; 184 Gründel (10.1016/j.ymben.2016.09.006_bib17) 2012; 158 Ruffing (10.1016/j.ymben.2016.09.006_bib52) 2012; 109 Pyne (10.1016/j.ymben.2016.09.006_bib47) 2015; 81 Shen (10.1016/j.ymben.2016.09.006_bib55) 2012; 5 Turan (10.1016/j.ymben.2016.09.006_bib57) 2013; 515 |
References_xml | – volume: 7 start-page: e47837 year: 2012 ident: bib8 article-title: Spatial and temporal organization of chromosome duplication and segregation in the cyanobacterium publication-title: PLoS One contributor: fullname: Savage – volume: 5 start-page: 9574 year: 2012 end-page: 9583 ident: bib55 article-title: Photosynthetic production of 2-methyl-1-butanol from CO publication-title: Energy Environ. Sci. contributor: fullname: Liao – volume: 151 start-page: 2605 year: 2005 end-page: 2613 ident: bib14 article-title: Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes publication-title: Microbiology contributor: fullname: Golden – volume: 32 start-page: 1262 year: 2014 end-page: 1267 ident: bib15 article-title: Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation publication-title: Nat. Biotechnol. contributor: fullname: Root – volume: 8 start-page: e56470 year: 2013 ident: bib54 article-title: CRISPR-Cas systems in the cyanobacterium Synechocystis sp. PCC 6803 exhibit distinct processing pathways involving at least two Cas6 and a Cmr2 protein publication-title: PLoS One contributor: fullname: Backofen – volume: 31 start-page: 233 year: 2013 end-page: 239 ident: bib23 article-title: RNA-guided editing of bacterial genomes using CRISPR-Cas systems publication-title: Nat. Biotechnol. contributor: fullname: Marraffini – volume: 47 start-page: 231 year: 2015 end-page: 243 ident: bib22 article-title: One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces publication-title: Acta Biochim. Biophys. Sin. (Shanghai) contributor: fullname: Lu – volume: 24 start-page: 556 year: 2016 end-page: 563 ident: bib3 article-title: In vivo CRISPR/Cas9 gene editing corrects retinal dystrophy in the S334ter-3 rat model of autosomal dominant retinitis pigmentosa publication-title: Mol. Ther. contributor: fullname: Wang – volume: 24 start-page: 430 year: 2016 end-page: 446 ident: bib35 article-title: Genome-editing technologies for gene and cell therapy publication-title: Mol. Ther. contributor: fullname: Gersbach – volume: 109 start-page: 2190 year: 2012 end-page: 2199 ident: bib52 article-title: Physiological effects of free fatty acid production in genetically engineered Synechococcus elongatus PCC 7942 publication-title: Biotechnol. Bioeng. contributor: fullname: Jones – volume: 30 start-page: 49 year: 2015 end-page: 60 ident: bib4 article-title: A state of the art of metabolic networks of unicellular microalgae and cyanobacteria for biofuel production publication-title: Metab. Eng. contributor: fullname: Bernard – year: 2016 ident: bib9 article-title: Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci publication-title: Nucleic Acids Res. contributor: fullname: Huang – volume: 44 start-page: 5615 year: 2016 end-page: 5628 ident: bib60 article-title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation publication-title: Nucleic Acids Res. contributor: fullname: Zoldos – volume: 152 start-page: 1173 year: 2013 end-page: 1183 ident: bib48 article-title: Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression publication-title: Cell contributor: fullname: Lim – volume: 81 start-page: 2506 year: 2015 end-page: 2514 ident: bib24 article-title: Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system publication-title: Appl. Environ. Microbiol contributor: fullname: Yang – volume: 158 start-page: 3032 year: 2012 end-page: 3043 ident: bib17 article-title: Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803 publication-title: Microbiology contributor: fullname: Zilliges – volume: 42 start-page: e131 year: 2014 ident: bib44 article-title: CRISPR-Cas9-assisted recombineering in publication-title: Nucleic Acids Res. contributor: fullname: van Pijkeren – volume: 157 start-page: 1262 year: 2014 end-page: 1278 ident: bib21 article-title: Development and applications of CRISPR-Cas9 for genome engineering publication-title: Cell contributor: fullname: Zhang – volume: 4 start-page: 1197 year: 2015 end-page: 1204 ident: bib43 article-title: Genome Engineering of the 2,3-butanediol biosynthetic pathway for tight regulation in cyanobacteria publication-title: ACS Synth. Biol. contributor: fullname: Atsumi – volume: 339 start-page: 819 year: 2013 end-page: 823 ident: bib12 article-title: Multiplex genome engineering using CRISPR/Cas systems publication-title: Science contributor: fullname: Zhang – volume: 337 start-page: 816 year: 2012 end-page: 821 ident: bib25 article-title: A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity publication-title: Science contributor: fullname: Charpentier – volume: 27 start-page: 1177 year: 2009 end-page: 1180 ident: bib2 article-title: Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde publication-title: Nat. Biotechnol. contributor: fullname: Liao – volume: 22 start-page: 76 year: 2014 end-page: 82 ident: bib46 article-title: Combinatorial optimization of cyanobacterial 2,3-butanediol production publication-title: Metab. Eng. contributor: fullname: Atsumi – volume: 81 start-page: 5103 year: 2015 end-page: 5114 ident: bib47 article-title: Coupling the CRISPR/Cas9 System with Lambda Red recombineering enables simplified chromosomal gene replacement in publication-title: Appl. Environ. Microbiol contributor: fullname: Chou – year: 2016 ident: bib10 article-title: Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9 publication-title: Biotechnol. Bioeng. contributor: fullname: Hu – volume: 110 start-page: 1249 year: 2013 end-page: 1254 ident: bib45 article-title: Cyanobacterial conversion of carbon dioxide to 2,3-butanediol publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Atsumi – volume: 8 start-page: 2180 year: 2013 end-page: 2196 ident: bib31 article-title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression publication-title: Nat. Protoc. contributor: fullname: Qi – volume: 183 start-page: 1740 year: 2001 end-page: 1747 ident: bib41 article-title: Functional elements of the strong psbAI promoter of Synechococcus elongatus PCC 7942 publication-title: J. Bacteriol. contributor: fullname: Golden – volume: 33 start-page: 8 year: 2015 end-page: 14 ident: bib53 article-title: Engineering cyanobacteria for direct biofuel production from CO publication-title: Curr. Opin. Biotechnol. contributor: fullname: Hellingwerf – volume: 52 start-page: 85 year: 1999 end-page: 95 ident: bib58 article-title: PCR assay for detection of the E. coli O157: h7 eae-gene and effect of the sample preparation method on PCR detection of heat-killed E. coli O157: h7 in ground beef publication-title: Int. J. Food Microbiol. contributor: fullname: Debevere – volume: 15 start-page: 1 year: 2016 end-page: 8 ident: bib62 article-title: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium publication-title: Microb. Cell Factor. contributor: fullname: Pakrasi – volume: 79 start-page: 1668 year: 2013 end-page: 1675 ident: bib38 article-title: Engineering Synechococcus elongatus PCC 7942 for continuous growth under diurnal conditions publication-title: Appl. Environ. Microbiol. contributor: fullname: Atsumi – volume: 34 start-page: 575 year: 2016 end-page: 587 ident: bib39 article-title: Next generation prokaryotic engineering: the CRISPR-Cas toolkit publication-title: Trends Biotechnol. contributor: fullname: van der Oost – volume: 520 start-page: 186 year: 2015 end-page: 191 ident: bib50 article-title: In vivo genome editing using Staphylococcus aureus Cas9 publication-title: Nature contributor: fullname: Zhang – volume: 41 start-page: 4336 year: 2013 end-page: 4343 ident: bib13 article-title: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems publication-title: Nucleic Acids Res. contributor: fullname: Church – volume: 112 start-page: 1060 year: 2015 end-page: 1064 ident: bib19 article-title: Highly efficient targeted chromosome deletions using CRISPR/Cas9 publication-title: Biotechnol. Bioeng. contributor: fullname: Lillico – volume: 80 start-page: 666 year: 2011 end-page: 677 ident: bib30 article-title: Gene conversion results in the equalization of genome copies in the polyploid haloarchaeon Haloferax volcanii publication-title: Mol. Microbiol. contributor: fullname: Soppa – volume: 323 start-page: 124 year: 2011 end-page: 131 ident: bib18 article-title: Ploidy in cyanobacteria publication-title: FEMS Microbiol. Lett. contributor: fullname: Soppa – volume: 20 start-page: 101 year: 2013 end-page: 108 ident: bib27 article-title: Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light publication-title: Metab. Eng. contributor: fullname: Hanai – volume: 32 start-page: 279 year: 2014 end-page: 284 ident: bib16 article-title: Improving CRISPR-Cas nuclease specificity using truncated guide RNAs publication-title: Nat. Biotechnol. contributor: fullname: Joung – volume: 163 start-page: 759 year: 2015 end-page: 771 ident: bib64 article-title: Cpf1 Is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system publication-title: Cell contributor: fullname: Zhang – volume: 4 start-page: 246 year: 2013 ident: bib5 article-title: Synthetic biology of cyanobacteria: unique challenges and opportunities publication-title: Front. Microbiol. contributor: fullname: Pakrasi – volume: 200 start-page: 1 year: 2015 end-page: 5 ident: bib61 article-title: Markerless chromosomal gene deletion in publication-title: J. Biotechnol. contributor: fullname: Blaschek – volume: 24 start-page: 636 year: 2016 end-page: 644 ident: bib40 article-title: CRISPR-Cas9 systems enable specific editing of the human genome publication-title: Mol. Ther. contributor: fullname: Mussolino – volume: 4 start-page: 1186 year: 2015 end-page: 1196 ident: bib49 article-title: Genome engineering in cyanobacteria: where we are and where we need to go publication-title: ACS Synth. Biol. contributor: fullname: Boyle – volume: 154 start-page: 1380 year: 2013 end-page: 1389 ident: bib51 article-title: Double nicking by RNA-Guided CRISPR Cas9 for enhanced genome editing specificity publication-title: Cell contributor: fullname: Zhang – volume: 76 start-page: 3153 year: 2010 end-page: 3159 ident: bib56 article-title: Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis publication-title: Appl. Environ. Microbiol contributor: fullname: Nakamura – volume: 12 start-page: 1051 year: 2015 end-page: 1054 ident: bib26 article-title: Cas9 gRNA engineering for genome editing, activation and repression publication-title: Nat. Methods contributor: fullname: Church – volume: 24 start-page: 564 year: 2016 end-page: 569 ident: bib63 article-title: CRISPR-mediated genome editing restores dystrophin expression and function in mdx mice publication-title: Mol. Ther. contributor: fullname: Han – volume: 515 start-page: 1 year: 2013 end-page: 27 ident: bib57 article-title: Recombinase-mediated cassette exchange (RMCE) – a rapidly-expanding toolbox for targeted genomic modifications publication-title: Gene contributor: fullname: Bode – volume: 339 start-page: 823 year: 2013 end-page: 826 ident: bib36 article-title: RNA-guided human genome engineering via Cas9 publication-title: Science contributor: fullname: Church – volume: 4 start-page: 4489 year: 2014 ident: bib34 article-title: CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori publication-title: Sci. Rep. contributor: fullname: Xia – volume: 109 start-page: 6018 year: 2012 end-page: 6023 ident: bib29 article-title: ATP drives direct photosynthetic production of 1-butanol in cyanobacteria publication-title: Proc. Natl. Acad. Sci. USA contributor: fullname: Liao – volume: 61 start-page: 335 year: 2005 end-page: 341 ident: bib6 article-title: Rapid detection of diarrheagenic E. coli by real-time PCR publication-title: J. Microbiol. Methods contributor: fullname: Baggi – volume: 31 start-page: 163 year: 2015 end-page: 170 ident: bib28 article-title: Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO publication-title: Metab. Eng. contributor: fullname: Liao – volume: 31 start-page: 13 year: 2015 end-page: 21 ident: bib32 article-title: Metabolic engineering of publication-title: Metab. Eng. contributor: fullname: Zhao – volume: 29 start-page: 160 year: 2015 end-page: 168 ident: bib33 article-title: Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: controllable P(3HB-co-4HB) biosynthesis publication-title: Metab. Eng. contributor: fullname: Chen – volume: 4 start-page: 723 year: 2015 end-page: 728 ident: bib11 article-title: High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system publication-title: ACS Synth. Biol. contributor: fullname: Zhao – volume: 147 start-page: 2077 year: 2001 end-page: 2087 ident: bib37 article-title: Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rpsl2 gene that allows selection of mutants free from drug resistance markers publication-title: Microbiology contributor: fullname: Ogawa – volume: 15 start-page: 2 year: 2016 ident: bib65 article-title: From cyanochemicals to cyanofactories: a review and perspective publication-title: Microb. Cell Factor. contributor: fullname: Li – volume: 33 start-page: 352 year: 2015 end-page: 361 ident: bib1 article-title: Metabolic engineering of cyanobacteria for the synthesis of commodity products publication-title: Trends Biotechnol. contributor: fullname: Hellingwerf – volume: 184 start-page: 100 year: 2014 end-page: 102 ident: bib59 article-title: Carbon sink removal: increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant publication-title: J. Biotechnol. contributor: fullname: Hellingwerf – volume: 76 start-page: 3462 year: 2010 end-page: 3466 ident: bib42 article-title: Engineering cyanobacteria to synthesize and export hydrophilic products publication-title: Appl. Environ. Microbiol contributor: fullname: Way – volume: 5 start-page: 9457 year: 2012 end-page: 9461 ident: bib7 article-title: Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage publication-title: Energy Environ. Sci. contributor: fullname: Yu – volume: 193 start-page: 734 year: 2011 end-page: 743 ident: bib20 article-title: Genome copy numbers and gene conversion in methanogenic archaea publication-title: J. Bacteriol. contributor: fullname: Soppa – volume: 81 start-page: 2506 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib24 article-title: Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system publication-title: Appl. Environ. Microbiol doi: 10.1128/AEM.04023-14 contributor: fullname: Jiang – volume: 27 start-page: 1177 year: 2009 ident: 10.1016/j.ymben.2016.09.006_bib2 article-title: Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde publication-title: Nat. Biotechnol. doi: 10.1038/nbt.1586 contributor: fullname: Atsumi – volume: 323 start-page: 124 year: 2011 ident: 10.1016/j.ymben.2016.09.006_bib18 article-title: Ploidy in cyanobacteria publication-title: FEMS Microbiol. Lett. doi: 10.1111/j.1574-6968.2011.02368.x contributor: fullname: Griese – volume: 515 start-page: 1 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib57 article-title: Recombinase-mediated cassette exchange (RMCE) – a rapidly-expanding toolbox for targeted genomic modifications publication-title: Gene doi: 10.1016/j.gene.2012.11.016 contributor: fullname: Turan – volume: 200 start-page: 1 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib61 article-title: Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system publication-title: J. Biotechnol. doi: 10.1016/j.jbiotec.2015.02.005 contributor: fullname: Wang – volume: 4 start-page: 723 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib11 article-title: High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system publication-title: ACS Synth. Biol. doi: 10.1021/sb500351f contributor: fullname: Cobb – volume: 31 start-page: 13 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib32 article-title: Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing publication-title: Metab. Eng. doi: 10.1016/j.ymben.2015.06.006 contributor: fullname: Li – volume: 24 start-page: 556 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib3 article-title: In vivo CRISPR/Cas9 gene editing corrects retinal dystrophy in the S334ter-3 rat model of autosomal dominant retinitis pigmentosa publication-title: Mol. Ther. doi: 10.1038/mt.2015.220 contributor: fullname: Bakondi – volume: 34 start-page: 575 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib39 article-title: Next generation prokaryotic engineering: the CRISPR-Cas toolkit publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2016.02.004 contributor: fullname: Mougiakos – volume: 24 start-page: 636 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib40 article-title: Streptococcus thermophilus CRISPR-Cas9 systems enable specific editing of the human genome publication-title: Mol. Ther. doi: 10.1038/mt.2015.218 contributor: fullname: Muller – volume: 33 start-page: 8 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib53 article-title: Engineering cyanobacteria for direct biofuel production from CO2 publication-title: Curr. Opin. Biotechnol. doi: 10.1016/j.copbio.2014.09.007 contributor: fullname: Savakis – volume: 24 start-page: 564 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib63 article-title: CRISPR-mediated genome editing restores dystrophin expression and function in mdx mice publication-title: Mol. Ther. doi: 10.1038/mt.2015.192 contributor: fullname: Xu – volume: 109 start-page: 2190 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib52 article-title: Physiological effects of free fatty acid production in genetically engineered Synechococcus elongatus PCC 7942 publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.24509 contributor: fullname: Ruffing – volume: 32 start-page: 1262 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib15 article-title: Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation publication-title: Nat. Biotechnol. doi: 10.1038/nbt.3026 contributor: fullname: Doench – volume: 112 start-page: 1060 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib19 article-title: Highly efficient targeted chromosome deletions using CRISPR/Cas9 publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.25490 contributor: fullname: He – volume: 81 start-page: 5103 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib47 article-title: Coupling the CRISPR/Cas9 System with Lambda Red recombineering enables simplified chromosomal gene replacement in Escherichia coli publication-title: Appl. Environ. Microbiol doi: 10.1128/AEM.01248-15 contributor: fullname: Pyne – year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib10 article-title: Enhanced integration of large DNA into E. coli chromosome by CRISPR/Cas9 publication-title: Biotechnol. Bioeng. contributor: fullname: Chung – volume: 33 start-page: 352 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib1 article-title: Metabolic engineering of cyanobacteria for the synthesis of commodity products publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2015.03.009 contributor: fullname: Angermayr – volume: 7 start-page: e47837 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib8 article-title: Spatial and temporal organization of chromosome duplication and segregation in the cyanobacterium Synechococcus elongatus PCC 7942 publication-title: PLoS One doi: 10.1371/journal.pone.0047837 contributor: fullname: Chen – volume: 184 start-page: 100 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib59 article-title: Carbon sink removal: increased photosynthetic production of lactic acid by Synechocystis sp. PCC6803 in a glycogen storage mutant publication-title: J. Biotechnol. doi: 10.1016/j.jbiotec.2014.04.029 contributor: fullname: van der Woude – volume: 22 start-page: 76 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib46 article-title: Combinatorial optimization of cyanobacterial 2,3-butanediol production publication-title: Metab. Eng. doi: 10.1016/j.ymben.2014.01.001 contributor: fullname: Oliver – volume: 158 start-page: 3032 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib17 article-title: Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803 publication-title: Microbiology doi: 10.1099/mic.0.062950-0 contributor: fullname: Gründel – volume: 4 start-page: 1197 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib43 article-title: Genome Engineering of the 2,3-butanediol biosynthetic pathway for tight regulation in cyanobacteria publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.5b00057 contributor: fullname: Nozzi – year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib9 article-title: Expanding the CRISPR imaging toolset with Staphylococcus aureus Cas9 for simultaneous imaging of multiple genomic loci publication-title: Nucleic Acids Res. contributor: fullname: Chen – volume: 147 start-page: 2077 year: 2001 ident: 10.1016/j.ymben.2016.09.006_bib37 article-title: Gene replacement in cyanobacteria mediated by a dominant streptomycin-sensitive rpsl2 gene that allows selection of mutants free from drug resistance markers publication-title: Microbiology doi: 10.1099/00221287-147-8-2077 contributor: fullname: Matsuoka – volume: 109 start-page: 6018 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib29 article-title: ATP drives direct photosynthetic production of 1-butanol in cyanobacteria publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1200074109 contributor: fullname: Lan – volume: 79 start-page: 1668 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib38 article-title: Engineering Synechococcus elongatus PCC 7942 for continuous growth under diurnal conditions publication-title: Appl. Environ. Microbiol. doi: 10.1128/AEM.03326-12 contributor: fullname: McEwen – volume: 4 start-page: 246 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib5 article-title: Synthetic biology of cyanobacteria: unique challenges and opportunities publication-title: Front. Microbiol. doi: 10.3389/fmicb.2013.00246 contributor: fullname: Berla – volume: 15 start-page: 2 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib65 article-title: From cyanochemicals to cyanofactories: a review and perspective publication-title: Microb. Cell Factor. doi: 10.1186/s12934-015-0405-3 contributor: fullname: Zhou – volume: 30 start-page: 49 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib4 article-title: A state of the art of metabolic networks of unicellular microalgae and cyanobacteria for biofuel production publication-title: Metab. Eng. doi: 10.1016/j.ymben.2015.03.019 contributor: fullname: Baroukh – volume: 61 start-page: 335 year: 2005 ident: 10.1016/j.ymben.2016.09.006_bib6 article-title: Rapid detection of diarrheagenic E. coli by real-time PCR publication-title: J. Microbiol. Methods doi: 10.1016/j.mimet.2004.12.007 contributor: fullname: Bischoff – volume: 52 start-page: 85 year: 1999 ident: 10.1016/j.ymben.2016.09.006_bib58 article-title: PCR assay for detection of the E. coli O157: h7 eae-gene and effect of the sample preparation method on PCR detection of heat-killed E. coli O157: h7 in ground beef publication-title: Int. J. Food Microbiol. doi: 10.1016/S0168-1605(99)00132-4 contributor: fullname: Uyttendaele – volume: 163 start-page: 759 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib64 article-title: Cpf1 Is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system publication-title: Cell doi: 10.1016/j.cell.2015.09.038 contributor: fullname: Zetsche – volume: 337 start-page: 816 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib25 article-title: A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity publication-title: Science doi: 10.1126/science.1225829 contributor: fullname: Jinek – volume: 76 start-page: 3462 year: 2010 ident: 10.1016/j.ymben.2016.09.006_bib42 article-title: Engineering cyanobacteria to synthesize and export hydrophilic products publication-title: Appl. Environ. Microbiol doi: 10.1128/AEM.00202-10 contributor: fullname: Niederholtmeyer – volume: 76 start-page: 3153 year: 2010 ident: 10.1016/j.ymben.2016.09.006_bib56 article-title: Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis publication-title: Appl. Environ. Microbiol doi: 10.1128/AEM.00397-08 contributor: fullname: Suzuki – volume: 5 start-page: 9574 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib55 article-title: Photosynthetic production of 2-methyl-1-butanol from CO2 in cyanobacterium Synechococcus elongatus PCC7942 and characterization of the native acetohydroxyacid synthase publication-title: Energy Environ. Sci. doi: 10.1039/c2ee23148d contributor: fullname: Shen – volume: 31 start-page: 163 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib28 article-title: Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942 publication-title: Metab. Eng. doi: 10.1016/j.ymben.2015.08.002 contributor: fullname: Lan – volume: 183 start-page: 1740 year: 2001 ident: 10.1016/j.ymben.2016.09.006_bib41 article-title: Functional elements of the strong psbAI promoter of Synechococcus elongatus PCC 7942 publication-title: J. Bacteriol. doi: 10.1128/JB.183.5.1740-1747.2001 contributor: fullname: Nair – volume: 29 start-page: 160 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib33 article-title: Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: controllable P(3HB-co-4HB) biosynthesis publication-title: Metab. Eng. doi: 10.1016/j.ymben.2015.03.013 contributor: fullname: Lv – volume: 8 start-page: 2180 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib31 article-title: CRISPR interference (CRISPRi) for sequence-specific control of gene expression publication-title: Nat. Protoc. doi: 10.1038/nprot.2013.132 contributor: fullname: Larson – volume: 4 start-page: 1186 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib49 article-title: Genome engineering in cyanobacteria: where we are and where we need to go publication-title: ACS Synth. Biol. doi: 10.1021/acssynbio.5b00043 contributor: fullname: Ramey – volume: 157 start-page: 1262 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib21 article-title: Development and applications of CRISPR-Cas9 for genome engineering publication-title: Cell doi: 10.1016/j.cell.2014.05.010 contributor: fullname: Hsu Patrick – volume: 41 start-page: 4336 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib13 article-title: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkt135 contributor: fullname: DiCarlo – volume: 151 start-page: 2605 year: 2005 ident: 10.1016/j.ymben.2016.09.006_bib14 article-title: Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes publication-title: Microbiology doi: 10.1099/mic.0.28030-0 contributor: fullname: Ditty – volume: 44 start-page: 5615 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib60 article-title: Repurposing the CRISPR-Cas9 system for targeted DNA methylation publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkw159 contributor: fullname: Vojta – volume: 520 start-page: 186 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib50 article-title: In vivo genome editing using Staphylococcus aureus Cas9 publication-title: Nature doi: 10.1038/nature14299 contributor: fullname: Ran – volume: 339 start-page: 819 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib12 article-title: Multiplex genome engineering using CRISPR/Cas systems publication-title: Science doi: 10.1126/science.1231143 contributor: fullname: Cong – volume: 42 start-page: e131 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib44 article-title: CRISPR-Cas9-assisted recombineering in Lactobacillus reuteri publication-title: Nucleic Acids Res. doi: 10.1093/nar/gku623 contributor: fullname: Oh – volume: 20 start-page: 101 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib27 article-title: Engineering a synthetic pathway in cyanobacteria for isopropanol production directly from carbon dioxide and light publication-title: Metab. Eng. doi: 10.1016/j.ymben.2013.09.007 contributor: fullname: Kusakabe – volume: 15 start-page: 1 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib62 article-title: CRISPR/Cas9 mediated targeted mutagenesis of the fast growing cyanobacterium Synechococcus elongatus UTEX 2973 publication-title: Microb. Cell Factor. doi: 10.1186/s12934-016-0514-7 contributor: fullname: Wendt – volume: 339 start-page: 823 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib36 article-title: RNA-guided human genome engineering via Cas9 publication-title: Science doi: 10.1126/science.1232033 contributor: fullname: Mali – volume: 80 start-page: 666 year: 2011 ident: 10.1016/j.ymben.2016.09.006_bib30 article-title: Gene conversion results in the equalization of genome copies in the polyploid haloarchaeon Haloferax volcanii publication-title: Mol. Microbiol. doi: 10.1111/j.1365-2958.2011.07600.x contributor: fullname: Lange – volume: 24 start-page: 430 year: 2016 ident: 10.1016/j.ymben.2016.09.006_bib35 article-title: Genome-editing technologies for gene and cell therapy publication-title: Mol. Ther. doi: 10.1038/mt.2016.10 contributor: fullname: Maeder – volume: 8 start-page: e56470 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib54 article-title: CRISPR-Cas systems in the cyanobacterium Synechocystis sp. PCC 6803 exhibit distinct processing pathways involving at least two Cas6 and a Cmr2 protein publication-title: PLoS One doi: 10.1371/journal.pone.0056470 contributor: fullname: Scholz – volume: 110 start-page: 1249 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib45 article-title: Cyanobacterial conversion of carbon dioxide to 2,3-butanediol publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1213024110 contributor: fullname: Oliver – volume: 152 start-page: 1173 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib48 article-title: Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression publication-title: Cell doi: 10.1016/j.cell.2013.02.022 contributor: fullname: Qi – volume: 31 start-page: 233 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib23 article-title: RNA-guided editing of bacterial genomes using CRISPR-Cas systems publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2508 contributor: fullname: Jiang – volume: 4 start-page: 4489 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib34 article-title: CRISPR/Cas9 mediated multiplex genome editing and heritable mutagenesis of BmKu70 in Bombyx mori publication-title: Sci. Rep. doi: 10.1038/srep04489 contributor: fullname: Ma – volume: 193 start-page: 734 year: 2011 ident: 10.1016/j.ymben.2016.09.006_bib20 article-title: Genome copy numbers and gene conversion in methanogenic archaea publication-title: J. Bacteriol. doi: 10.1128/JB.01016-10 contributor: fullname: Hildenbrand – volume: 5 start-page: 9457 year: 2012 ident: 10.1016/j.ymben.2016.09.006_bib7 article-title: Photo-catalytic conversion of carbon dioxide to organic acids by a recombinant cyanobacterium incapable of glycogen storage publication-title: Energy Environ. Sci. doi: 10.1039/c2ee23181f contributor: fullname: Carrieri – volume: 32 start-page: 279 year: 2014 ident: 10.1016/j.ymben.2016.09.006_bib16 article-title: Improving CRISPR-Cas nuclease specificity using truncated guide RNAs publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2808 contributor: fullname: Fu – volume: 47 start-page: 231 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib22 article-title: One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces publication-title: Acta Biochim. Biophys. Sin. (Shanghai) doi: 10.1093/abbs/gmv007 contributor: fullname: Huang – volume: 12 start-page: 1051 year: 2015 ident: 10.1016/j.ymben.2016.09.006_bib26 article-title: Cas9 gRNA engineering for genome editing, activation and repression publication-title: Nat. Methods doi: 10.1038/nmeth.3580 contributor: fullname: Kiani – volume: 154 start-page: 1380 year: 2013 ident: 10.1016/j.ymben.2016.09.006_bib51 article-title: Double nicking by RNA-Guided CRISPR Cas9 for enhanced genome editing specificity publication-title: Cell doi: 10.1016/j.cell.2013.08.021 contributor: fullname: Ran |
SSID | ssj0011591 |
Score | 2.5561633 |
Snippet | Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived chemicals, but genome engineering of cyanobacteria such as Synechococcus... |
SourceID | proquest crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 293 |
SubjectTerms | Apoptosis - genetics Biosynthetic Pathways - genetics CRISPR-Cas Systems - genetics CRISPR-Cas9 Cyanobacteria Gene Editing - methods Genetic Enhancement - methods Genome editing Genome, Bacterial - genetics Metabolic engineering Metabolic Engineering - methods Metabolic Networks and Pathways - genetics PCC 7942 Succinate Succinic Acid - isolation & purification Succinic Acid - metabolism Synechococcus - cytology Synechococcus - physiology Synechococcus elongatus |
Title | CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production |
URI | https://dx.doi.org/10.1016/j.ymben.2016.09.006 https://www.ncbi.nlm.nih.gov/pubmed/27693320 https://search.proquest.com/docview/1835349563 |
Volume | 38 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8NAEB5si6AH8W19lBU8GptkH2mOEpRWsUir0Nuy2WygQtNi20Mv_nZn8_AB6sFjwi5ZZrLzfcPsfgNwEXYoklarUylCTFAEs0VC5jvKTUJqEaaT2PvOD33RfWZ3Iz5ag6i6C2OPVZaxv4jpebQu37RLa7Zn43F76CH7xmREIKOwtbFODRoIR4zVoXHdu-_2P4oJiNh53oXjHTuhEh_Kj3mtJrGxOqieyPVObeejnwHqNwKaA9HtNmyVDJJcF4vcgTWT7cJ60VNytQubXxQG9-AxGvSGjwMnUvOQID8lyPeI1WWdGGI-B5JpSvRKZbi5c_FmRVSWkPlS63GGZJTMCl1Y9OE-PN_ePEVdp2yi4GjcjgunQ1NMeQzStiSIFWJP4vI08A1LXIyDHtPcSzXyqEBrHWgeUJNwI2JELeQqHtr2AOrZNDNHQJBLcaUDjrDqMy2E4uhRFiKnoJSmwm_CZWU5OSu0MmR1iOxF5oaW1tDSDSUaugmisq785nKJ0fzvieeVLyRuBlvhUJmZLucS4xOnNuWjTTgsnPSxEt92faS-e_zfz57Ahn0qLiKeQn3xujRnyEgWcQtqV29eq_zv3gHhkdvS |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1LS8NAEMcHrYh6EN_W5woejU2y2U1zlKK0akupCt6W7WYDFZoW2x568bM7k4cPUA9ekw0JM9mZ37Kz_wE4j-ocoZV0KmWECxQZ0CZh4DvajSNOGaYe03nndkc2n4LbZ_G8AI3yLAyVVRaxP4_pWbQurtQKa9bGg0HtwUP6xsWIRKKgvbH6IiwRzlP_hsu3jzoPJJ6sbR6Ndmh4KT2UFXnNh31LKqiezNROqe_Rz-npN_zM0tDNBqwX_Miu8k_chAWbbsFy3lFyvgVrX_QFt6Hb6LUeuj2noScRQzplSHuMVFmHltnPgWyUMDPXKU7tTLpZM53GbDIzZpAiirJxrgqLHtyBp5vrx0bTKVooOAYn49Sp8wQXPBahLQ77GjNP7Iok9G0QuxgFvcAILzFIUaExJjQi5DYWVvYxZyGpeGjZXaiko9TuA0OSEtqEApOqHxgptUB_BhESBec8kX4VLkrLqXGulKHKErIXlRlakaGVGyk0dBVkaV31zeEKY_nfD56VvlA4FWh_Q6d2NJsojE6C04KPV2Evd9LHl_jU85H77sF_X3sKK83H9r26b3XuDmGV7uRHEo-gMn2d2WNkk2n_JPv33gG9rdyg |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=CRISPR-Cas9+for+the+genome+engineering+of+cyanobacteria+and+succinate+production&rft.jtitle=Metabolic+engineering&rft.au=Li%2C+Hung&rft.au=Shen%2C+Claire+R&rft.au=Huang%2C+Chun-Hung&rft.au=Sung%2C+Li-Yu&rft.date=2016-11-01&rft.eissn=1096-7184&rft.volume=38&rft.spage=293&rft_id=info:doi/10.1016%2Fj.ymben.2016.09.006&rft_id=info%3Apmid%2F27693320&rft.externalDocID=27693320 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1096-7176&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1096-7176&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1096-7176&client=summon |